Mayank Roshan

National Dairy Research Institute

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Ashutosh Vats

The Pirbright Institute

Devika Gautam

National Dairy Research Institute

Sachinandan De

National Dairy Research Institute

Manisha Behera

Presidency University

Shiveeli Rajput

National Dairy Research Institute

Parmanand

National Dairy Research Institute

Chanchal Rana

National Dairy Research Institute

Manoj Kumar Singh

National Dairy Research Institute

Ashutosh Ludri

National Dairy Research Institute

Sachinandan De

National Dairy Research Institute

Cooperative Institutions

National Dairy Research Institute

Indian Council of Agricultural Research

Indian Veterinary Research Institute

All India Institute of Medical Sciences

National Bureau of Animal Genetic Resources

University of Delhi

Central Institute for Research on Cattle

Chandigarh University

Banaras Hindu University

ICAR-National Research Centre on Pig

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Embryo and gamete donation: a legal analysis

DOAJ (DOAJ: Directory of Open Access Journals) (2007)

Mayank Roshan

Gamete

Embryo donation

Oocyte donation

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Homologous transplantation of fluorescently labelled enriched buffalo (Bubalus bubalis) spermatogonial stem cells to prepubertal recipients

The Indian Journal of Animal Sciences (2020)

Ankur Sharma A. Kumaresan Narendra Nala Manish Tiwari Mayank Roshan

Spermatogonial stem cell transplantation provides a unique opportunity to study the biology of spermatogenesis and also offers an alternative approach for genetic modification in large animals. The present study aimed to extend this technique to the water buffalo. Spermatogonial stem cells (SSCs) were isolated from prepubertal buffalo testes (3-6 months of age) using two-step enzymatic digestion method and enriched by differential plating and Percoll density gradient centrifugation. The enriched SSCs expressed numerous spermatogonial transcriptional markers, viz. ID4, THY1, BCL6B, UCHL1, ETV5 and REX1 which confirmed their bonafide SSC identity. Subsequently, the enriched SSCs were labelled with a fluorescent dye PKH26 and transplanted into buffalo calves under ultrasound guidance. The recipient testes were recovered after 7-8 weeks by castration and their fluorescence microscopebased examination exhibited the persistence and localization of the fluorescent donor cells within the recipient seminiferous tubules. Further validation was done by the flow cytometric evaluation of PKH26 labeled donor cells among those isolated by two-step enzymatic digestion of recipient testicular parenchyma. In conclusion, we demonstrated the feasibility of SSC transplantation technique in the water buffalo.

Differential centrifugation

Percoll

Parenchyma

Bubalus

10.56093/ijans.v90i5.104608

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Citations (1)

Adaptive Selection in the Evolution of Aquaglyceroporins in Mammals

Journal of Molecular Evolution (2023)

Shiveeli Rajput Devika Gautam Ashutosh Vats Chanchal Rana Manisha Behera

Subfamily

Molecular evolution

Conserved sequence

10.1007/s00239-023-10112-5

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Poly(I:C), a double stranded RNA analog, activates the anti-viral DNA sensors in buffalo fibroblasts

Veterinary Vaccine (2023)

Jatinder Singh Chera Sushil Kumar Amit Kumar Bairagi Amit Kumar Chandra Prakash

Polyinosinic:polycytidylic acid or poly(I:C) is a double stranded RNA analog that is known for stimulating RNA sensing pathways in a variety of cell culture and animal models. Activation of nucleic acid sensing pathways lead to the up-regulation of Interferon-β which ultimately creates an antiviral state in the host. The involvement of intracellular DNA sensors in poly(I:C) mediated immune response has not been extensively explored. Poly(I:C) stimulates the intracellular DNA sensing in addition to RNA sensing pathways in buffalo fibroblasts. Genomic DNA leak into the cytosol due to poly(I:C) is likely to stimulate the intracellular DNA sensing pathways. The mRNA expression of DNA sensors cGAS, IFI16-L (IFI16-like) and DAI are elevated while DDX41 and STING are down-regulated in poly(I:C) treated cells. However, STING activation is evident in poly(I:C) treated cells through the formation of aggregates around the nucleus. IFI16-L also aggregates like STING and translocates from the nucleus to the cytoplasm in response to poly(I:C). The activation of DNA sensors by a dsRNA mimic indicates that mammalian cells can use their own molecules for defending themselves against pathogenic RNA viruses. This provides an opportunity to search for novel therapeutic targets against RNA viruses that evade detection by canonical pattern recognition receptors.

RNA Silencing

10.1016/j.vetvac.2023.100016

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Virulence and enterotoxin gene profile of methicillin-resistant Staphylococcus aureus isolates from bovine mastitis

Comparative Immunology Microbiology and Infectious Diseases (2021)

Mayank Roshan Parmanand Devan Arora Manisha Behera Ashutosh Vats

SCCmec

10.1016/j.cimid.2021.101724

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Citations (13)

Green synthesis of silver oxide nanoparticles using Trigonella foenum-graecum leaf extract and their characterization

Semiconductor Physics Quantum Electronics & Optoelectronics (2024)

M. Irfana Amrin Mayank Roshan R. SaiGowri S. C. Vella Durai

The unique physical and chemical properties of silver nanoparticles (Ag NPs) of different sizes and shapes made their synthesis expedient. The most important method of NPs synthesis is the chemical process. However, the disadvantages of this method are the need for specific conditions such as high temperatures, to ensure formation and stability of NPs, as well as use of heavy aromatic solvents. Biosynthesis of NPs is considered advantageous over the traditional chemical approach. In this paper, the first report of the synthesis of silver oxide (AgO) NPs using Trigonella foenum-graecum leaf extract as a reducing agent is presented. The NPs were characterized by X-ray diffraction (XRD), thermogravimetric analysis/differential thermal analysis (TA/DTA), UV, photolumines-cence, SEM, EDX and high resolution transmission electron microscopy (HRTEM). The XRD confirmed the formation of high-purity AgO fine crystals. The average crystal size ranged from 27 to 32 nm as was revealed by HRTEM. From the Tauc plot, the optical band gap of the AgO crystals of 3.3 eV was determined. Thermal analysis provided the optimum temperature for calcination of the AgO NPs to be 400 °C.

Trigonella

Silver nanoparticle

Characterization

10.15407/spqeo27.02.162

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Citations (2)

Characterization and expression profiling of buffalo IFN-lambda family

Veterinary Immunology and Immunopathology (2024)

Devika Gautam Anil Sindhu Ashutosh Vats Shiveeli Rajput Mayank Roshan

Multiplicity of infection

Interferon type I

10.1016/j.vetimm.2024.110770