Xing Yujun

Ministry of Agriculture and Rural Affairs

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Qin Ji

University of Oxford

Qing Yang

Henan Academy of Agricultural Sciences

Dai Chengcheng

Huaiyin Institute of Technology

MA Chang-zhong

Tibet University

Xuelian Wu

State Grid Corporation of China (China)

Deji Ciren

Tibet Academy of Agricultural and Animal Husbandry Sciences

Yuming Luo

Huaiyin Normal University

Xiaojun Yang

Sun Yat-sen University

Fei Dong

Ministry of Agriculture and Rural Affairs

Sun Fuyao

Ministry of Agriculture and Rural Affairs

Cooperative Institutions

Ministry of Agriculture and Rural Affairs

Nanjing Agricultural University

Tibet Academy of Agricultural and Animal Husbandry Sciences

Jiangsu Academy of Agricultural Sciences

Huaiyin Normal University

University of KwaZulu-Natal

Huazhong Agricultural University

Central South University

Chinese Academy of Agricultural Sciences

Tibet University

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Optimization of Agrobacterium-mediated transformation parameters for sweet potato embryogenic callus using -glucuronidase (GUS) as a reporter

AFRICAN JOURNAL OF BIOTECHNOLOGY (2007)

Xing Yujun Qing Yang Qin Ji Yuming Luo Zhang Yun-feng

Agrobacterium-mediated transformation factors for sweet potato embryogenic calli were optimized using β-glucuronidase (GUS) as a reporter. The binary vector pTCK303 harboring the modified GUS gene driven by the CaMV 35S promoter was used. Transformation parameters were optimized including bacterial concen-tration, pre-culture period, co-cultivation period, immersion time, acetosyringone (AS) concentration and mannitol treated time. Results were obtained based on the percentage of GUS expression. Agrobacterium tumefaciens strain EHA105 at concentration OD600 nm = 0.8 showed the highest virulence on sweet potato embryogenic callus. Four days of pre-culture, four days of co-cultivation, 10 min of immersion, 200 μM acetosyringone and 60 min of mannitol-treated embryogenic callus gave the highest percentage of GUS positive transformants. Key words: Agrobacterium-mediated, transformation parameters, sweet potato embryogenic callus, β-glucuronidase.

Acetosyringone

Callus

Beta-glucuronidase

Glucuronidase

GUS reporter system

10.5897/ajb2007.000-2411

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Studies on Agrobacterium-mediated genetic transformation of embryogenic suspension cultures of sweet potato

AFRICAN JOURNAL OF BIOTECHNOLOGY (2008)

Xing Yujun Qin Ji Qing Yang Yuming Luo Qiang Li

In this study, genetic transformation of embryogenic suspension cultures of sweet potato (Ipomoea batatas) cultivar Xu55-2 was conducted utilizing the Agrobacterium tumefaciens strain EHA105 that contains the binary vector pBIN19/SBD2 with SBD2 (starch binding domain 2) gene and neomycin phosphotransferase (NPT II) gene. The presence of the SBD2 gene in the genomic DNA of transgenic plants was verified by PCR amplification and confirmed by Southern blot analysis. Results suggested that cefotaxime (Cefo), at the concentration of 200 mg/L, was able to effectively suppress the growth of Agrobacterium after co-cultivation. The optimal concentration for kanamycin (Kan) was 10 mg/L for selecting resistance calli, somatic embryo formation and plant regeneration. The highest frequency of shoot induction (30.9%) was obtained on the MS medium containing 10 mg/L Kan, 200 mg/L Cefo, 1.0 mg/L abscisic acid (ABA) and 1.0 mg/L gibberellic acid (GA3).

Kanamycin

Gibberellic acid

Callus

Southern blot

Ipomoea

10.5897/ajb07.724

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Protective function of pharmacy intravenous admixture service to nursing staff

Tianjin Journal of Nursing (2004)

Xing Yujun

Medical staff have to deal with the hazardous drugs each day, therefore, it is very important to prevent the harm and pollution by understanding the way of hazardous drug's transmission absorption. With the medical development, the specific prevention of medical staff has been greatly improved. The establishment of PIVAS center will tremendously protect medical staff from the harm during their work.

Nursing staff

Intravenous drug

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Characterization of Fusarium Species Causing Head Blight of Highland Barley (Qingke) in Tibet, China

Sun Fuyao Tangwei Zhang Xing Yujun Dai Chengcheng Deji Ciren

Download This Paper Open PDF in Browser Add Paper to My Library Share: Permalink Using these links will ensure access to this page indefinitely Copy URL Copy DOI

10.2139/ssrn.4671030

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Function of the Mitochondrial Transport Protein BcMtp1 in Regulating Vegetative Development, Asexual Reproduction, Stress Response, Fungicide Sensitivity, and Virulence of Botrytis cinerea

Journal of Fungi (2022)

Wenyong Shao Yu Zhang Changjun Chen Xing Yujun

In model fungi, mitochondrial transport proteins (MTPs), also known as “mitochondrial carriers” (MC), are known to facilitate the exchange of biochemical substances across the mitochondrial inner membrane. In this study, we characterized an MTP in Botrytis cinerea homologous to the known MTPs in Saccharomyces cerevisiae designated BcMtp1. The BcMtp1 deletion mutant phenotype was strikingly defective in vegetative development, conidiation, and sclerotia production. In addition, ΔBcMtp1 showed increased sensitivity to osmotic stress, oxidative stress, and cell wall biogenesis inhibitors. In the pathogenicity assay, ΔBcMtp1 displayed compromised virulence on various host-plant tissues. The BcMtp1 deletion mutant phenotype was rescued by transforming the wild-type BcMtp1 variant into the mutant. Together, these data indicate that BcMtp1 is critical for vegetative development, asexual reproduction, stress tolerance, and virulence of B. cinerea.

Conidiation

Asexual reproduction

10.3390/jof9010025

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In vitro Screening of Resistant Potato Clones to Late Blight with Crude Phytophthora infestans Toxin

Xing Yujun

The leaf explants of three potato varieties were used as the subject materials to in vitro screen so-matically variant clones of potato by means of crude Phytophthora infestans toxin and the results showed that the leaves and calluses of different varieties had different tolerating capacities to the toxin. The maximum tolerant concentration of Favorite to the toxin was 40% and those of Dongnong 303 and Zaod-abai were 50%. Crude Phytophthora infestans toxin showed significant inhibitory effects on callus induction and growth,adventitious bud differentiation and regenerated-plantlet rooting,and the inhibitory effects become intensified with the toxin concentration increased. The cellular clones screened by means of crude Phytophthora infestans toxin as well as their regenerated plantlets were tested to have a significantly higher resisting capacity at a high toxin concentration than the controls.

Phytophthora infestans

Plantlet

Callus

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Expression of an engineered tandem-repeat starch-binding domain in sweet potato plants

AFRICAN JOURNAL OF BIOTECHNOLOGY (2013)

Yunfeng Zhang Qin Ji Xing Yujun Qiang Li Xin Wang

In this study, the transgenic sweet potato Xu55-2 modified with an engineered tandem repeat of a family 20 starch binding domain (SBD2) was analyzed by Western dot blot to investigate whether SBD2 proteins are capable of granule-targeting during starch biosynthesis. Furthermore, the impact of SBD2 accumulation in granules on the physicochemical properties of the transgenic starches was also investigated. Our results demonstrate that the high levels of SBD2 protein could be accumulated in granules. The SBD2 expression affect granule morphology without altering the primary structure of the constituent starch molecules, suggesting that SBD2 could be used as an anchor for effector proteins to sweet potato starch granules during biosynthesis. Key words: Sweet potato (Ipomoea batatas), tandem starch-binding domain, transgenic starch, granule morphology.

Ipomoea

Granule (geology)

Tandem

Potato starch

10.5897/ajb11.3321

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Citations (2)

Research Progress in Genetic Transformation of Sweet Potato Mediated by Agrobacterium tumefaciens

Anhui nongye kexue (2007)

Xing Yujun

It is an important way to obtain transgenic sweet potato plants by Agrobacterium tumefaciens-mediated transformation.In this review the principle of the transformation mediated by Agrobacterium tumefaciens was described,the effect of factors including genotypes of sweet potato,kind of explants,density of agrobacterium culture etc on the transformation efficiency of sweet potato was discussed,and the development and prospect of genetic sweet potato obtained by Agrobacterium tumefaciens-mediated transformation were discussed.

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Characterization of Fusarium species causing head blight of highland barley (qingke) in Tibet, China

International Journal of Food Microbiology (2024)

Sun Fuyao Zhang Tangwei Xing Yujun Dai Chengcheng Deji Ciren

10.1016/j.ijfoodmicro.2024.110728

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Citations (1)