James J. Fischer

Research Field

A technique is described for opening the membrane of a red blood cell by electroporation in a manner which permits free exchange of the native hemoglobin with exogenous hemoglobin in the surrounding medium. After resealing the RBC's demonstrate near normal size and hemoglobin content and retain an effective methemoglobin reduction system. This method can be used to introduce natural or genetically engineered hemoglobins with altered oxygen binding characteristics. Allosteric effectors and other non-diffusible small molecules can be encapsulated during the same procedure. A fish Root effect hemoglobin exchanged into rat RBC's produced oxygen transport characteristics, unloading at high pressure at acidic pH, which should be useful to treat tissue hypoxia from a variety of causes.

Methemoglobin

Oxygen transport

10.3109/10731199809117679

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Citations (3)

Effects of hyperbaric oxygen and a perfluorooctylbromide emulsion on the radiation responses of tumors and normal tissues in rodents

International Journal of Radiation Oncology*Biology*Physics (1992)

Sara Rockwell Carolyn G. Irvin Marianne Kelley Christine Hughes Hiroko Yabuki

Radiosensitivity

Radiosensitizer

10.1016/0360-3016(92)90986-r

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Citations (30)

Enhancement of tumor radiation response by the combination of a perfluorochemical emulsion and hyperbaric oxygen

International Journal of Radiation Oncology*Biology*Physics (1987)

Douglas F. Martin Elizabeth Porter Sara Rockwell James J. Fischer

Radioresistance

Radiosensitivity

Limiting

10.1016/0360-3016(87)90294-x

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Citations (51)

Differential effect of cycloheximide on normal tissue tolerance and tumor control in irradiated rats

International Journal of Radiation Oncology*Biology*Physics (1984)

Joseph B. Weissberg James J. Fischer John E. Moulder Diana Fischer

Differential effects

Hindlimb

10.1016/0360-3016(84)90180-9

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Citations (2)

The Cure of Rhabdomyosarcoma BA 1112 with Fractionated Radiotherapy

Radiology (1972)

James J. Fischer H Reinhold

The experimentally determined time-dose isoeffect relationship for cure of the rat rhabdomyosarcoma BA 1112 is presented. In marked contrast to what might be anticipated for a tumor of cells showing unusually prominent Elkind-type recovery (Dq = 870 rads for hypoxic cells), there is little change in the TCD50 for fractionation courses of as many as ten separate treatments. A mathematical model making use of the previously established radiobiological parameters of this tumor and containing a provision for reoxygenation of initially hypoxic tumor cells during the fractionated treatment successfully predicts the observed time-dose relationship.

10.1148/105.2.429

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Citations (16)

A simple technique for irradiation of recto-anal cancers with electrons using an internal anal shield

International Journal of Radiation Oncology*Biology*Physics (1991)

Jeffery D. Morton Ravinder Nath Ali S. Meigooni James J. Fischer

Anus

Anal Carcinoma

10.1016/0360-3016(91)90295-f

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Citations (1)

Radiation reaction of rat skin.The role of the number of fractions and the overall treatment time

Cancer (1976)

John E. Moulder James J. Fischer

Acute skin reactions resulting from orthovoltage x-irradiation were measured in the WAG/Rij rat. A wide range of combinations of fraction number and overall time were used in an attempt to determine their relative importance. There appeared to be little effect of prolongation of overall treatment time until periods of over 2 weeks were reached. In treatment periods of 3 weeks and over the stimulation of extensive time-dependent recovery was seen. A model based on cell kill with provision for the stimulation of accelerated proliferation at about 17 days adequately describes the experimental results.

Skin reaction

10.1002/1097-0142(197606)37:6<2762::aid-cncr2820370629>3.0.co;2-o

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Citations (60)

121 Preliminary results of a randomized trial of mitomycin C as an adjunct to radical radiotherapy in the treatment of locally advanced squamous cell carcinoma of the cervix

International Journal of Radiation Oncology*Biology*Physics (1996)

Kenneth B. Roberts Nelson Urdaneta Raúl R. Vera Andrés Vera Enrique Javier Díez Gutiérrez

Adjunct

Mitomycin C

10.1016/s0360-3016(97)85462-4

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Citations (0)