Joaquim Coutinho Netto

Universidade de São Paulo

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João José Lachat

Universidade de São Paulo

Wagner Ferreira dos Santos

Universidade de São Paulo

Marco Andrey Cipriani Frade

Universidade de São Paulo

Norma Tiraboschi Foss

Universidade de São Paulo

José Antônio Thomazini

Universidade do Vale do Sapucaí

Andréia C. K. Fontana

Drexel University

Carlos Eli Piccinato

Universidade de São Paulo

Eduardo Lopez Mazzucato

Universidade Brasil

ÍB

Ígor Brum Cursi

Universidade do Estado do Rio de Janeiro

Felipe Fortes Andrade

Universidade Federal de Juiz de Fora

Cooperative Institutions

Universidade de São Paulo

122

Universidade de Ribeirão Preto

Clinics Hospital of Ribeirão Preto

Universidade Brasil

Fundação Faculdade de Medicina

Hospital Universitário da Universidade de São Paulo

Universidade Estadual Paulista (Unesp)

Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo

Universidade Estadual de Campinas (UNICAMP)

Universidade Federal de São Carlos

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Analysis of phospholipase A2, l‐amino acid oxidase, and proteinase enzymatic activities of the Lachesis muta rhombeata venom

Journal of Biochemical and Molecular Toxicology (2012)

Lucas Benício Campos Manuela Berto Pucca Eduardo Crosara Roncolato Joaquim Coutinho Netto José Elpídio Barbosa

Abstract The study of venom components is an important step toward understanding the mechanism of action of such venoms and is indispensable for the development of new therapies. This work aimed to investigate the venom of Lachesis muta rhombeata and evaluate enzymes related to its toxicity. Phospholipase A2 (PLA 2 ), l ‐amino acid oxidase (LAAO), and proteinase activities were measured, and the molecular weights were estimated. We found the venom to contain one PLA 2 (17 kDa), one LAAO (132 kDa), and three serine proteinases (40, 31, and 20 kDa). Although only serine proteinases were observed in the zymogram, metalloproteinases were found to contribute more to the total proteolytic activity than did serine proteinases. The work confirmed the presence of highly active enzymes; and, moreover, we proposed a novel method for confirming the presence of LAAOs by zymography. We also suggested a simple step to increase the sensitivity of proteinase assays. © 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:308–314, 2012; View this article online at wileyonlinelibrary.com. DOI 10:1002/jbt.21422

Zymography

Phospholipase A

10.1002/jbt.21422

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La biomembrana natural como una nueva propuesta para el tratamiento de úlceras de presión

Revista Medicina Cutánea Ibero-Latino-Americana (2006)

Marco Andrey Cipriani Frade Adriana Martinelli Salathiel Eduardo Lopez Mazzucato Joaquim Coutinho Netto Norma Tiraboschi Foss

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Influência da biomembrana de látex na indução e controle da cicatrização de áreas ulceradas extensas em modelos experimentais em coelhos

Eduardo Lopez Mazzucato Joaquim Coutinho Netto Marco Andrey Cipriani Frade

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Neovaginoplastia com molde de látex natural: uma nova proposta terapêutica

Luiz Gustavo Oliveira Brito Bruno Ramalho de Carvalho Milena Bastos Brito Ionara Diniz Evangelista Sousa Barcelos Antônio Alberto Nogueira

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Desenvolvimento de um sistema para medida da complacência de tubos de borracha natural fabricados pelo método de di-coating

S. Z. Sansavino Joaquim Coutinho Netto Marcelo Mulato Miguel Ferreira

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Biological activity and partial purification of brazilian tarantulas spiders venoms

Programa e Resumos (1995)

Wagner Ferreira dos Santos Andréia C. K. Fontana Sarah Lucas Joaquim Coutinho Netto

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Manejo de úlceras cutâneas diabéticas com a biomembrana natural de látex

Revista Medicina Cutánea Ibero-Latino-Americana (2004)

Marco Andrey Cipriani Frade Ígor Brum Cursi Felipe Fortes Andrade Joaquim Coutinho Netto Fábio Magalhães Barbetta

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Prótese vascular derivada do látex Latex-derived vascular prosthesis

Marcelo Luiz Lima Brandão Joaquim Coutinho Netto José Antônio Thomazini João José Lachat Valdair Francisco Muglia

Background: The development of vascular grafts has been crucial for advances and achievements in reconstructive vascular surgery over the past 5 decades. Objectives: To develop a new model of microperforated vascular graft using fabric covered with a natural latex-derived polymer taken from Hevea brasiliensis and assess its patency, thrombogenicity, biocompatibility and healing process, besides some mechanical properties (adaptability, elasticity, impermeability and possibility of suture), using expanded polytetrafluoroethylene graft as control. Methods: Fifteen dogs were divided into three groups of five animals. The microperforated latex graft was implanted in all dogs and the expanded polytetrafluoroethylene graft was implanted in the contralateral pelvic limb. Postoperative follow-up was 4, 8 and 12 weeks. Analysis of results was performed according to clinical evaluation of pulses, complications (fluid collection, dehiscence, granuloma and infection), arteriography, macroscopic analysis and scanning electron micrography.

Thrombogenicity

Vascular graft

Vascular surgery

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The vegetal biomembrane in the healing of chronic venous ulcers

Anais Brasileiros de Dermatologia (2012)

Marco Andrey Cipriani Frade Raimunda Violante Campos de Assis Joaquim Coutinho Netto Thiago Antônio Moretti de Andrade Norma Tiraboschi Foss

BACKGROUND: The vegetal biomembrane has been used to treat cutaneous ulcers. OBJECTIVES: To assess the role of the vegetal biomembrane on the chronic venous ulcers treatment compared to treatment with collagenase cream. METHODS: Fourteen patients were selected to be treated with vegetal biomembrane and 7 with Fibrase®(CONTROL), followed clinically and photographically by the Wound Healing Index by ImageJ during 120 days and biopsied on the 1st and 30th days for histological examination. RESULTS: The vegetal biomembrane was better in promoting healing of the ulcers, especially on the inflammatory phase, confirmed by abundant exudation and wound debridement than the CONTROL group, on the 30th day. There was a greater tendency to angiogenesis followed by re-epithelialization with highest wound healing index on the 90th and 120th days. CONCLUSION: A combined analysis of clinical and histopathological findings suggests that the vegetal biomembrane acted as a factor inducing wound healing, especially on the inflammatory phase, confirmed by abundant exudation of the lesions promoting the transformation of the microenvironment of the chronic venous ulcers, and also stimulating angiogenesis and subsequent re-epithelialization.

Debridement (dental)

Granulation tissue

10.1590/s0365-05962012000100005

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Citations (50)

Morphologic evaluation of the use of a latex prosthesis in videolaparoscopic inguinoplasty: an experimental study in dogs

Acta Cirúrgica Brasileira (2011)

Luiz Henrique de Sousa Reginaldo Ceneviva Joaquim Coutinho Netto Fátima Mrué Luiz Henrique de Sousa Filho

PURPOSE: To evaluate the morphological aspects of the behavior of 4 types of latex biomembranes implanted in preperitoneal videolaparoscopic inguinoplasty. METHODS: Sixteen inguinoplasties were performed in 12 dogs: group 1 received an impermeable latex biomembrane in the right inguinal region and a prolene prosthesis, as control, in the contralateral inguinal region; groups 2, 3 and 4 received latex biomembranes respectively containing impermeable polyamide, 1-mm thick porous polyamide and 0.5-mm thick porous polyamide. Macro- and microscopic evaluations of the inguinal region and of the removed implants were made on the 7th, 14th, 21st and 28th days in group 1 and on the 28th postoperative day in the other groups. RESULTS: We observed absence of hematoma, seroma and infection; presence of tortuosities; induction of vascular neoformation, inflammatory reaction and collagen deposition, and full encystment of the latex biomembranes, except that with fine porous polyamide, which was partially incorporated, with the formation of microcysts. No latex biomembrane induced fibrosis as observed in the prolene control group. CONCLUSIONS: The biomembranes maintain induction of the healing process without fibrosis, are fully encysted and, except for the one with fine porous polyamide, are not incorporated into adjacent tissues. The latex biomembrane, with or without polyamide, is not recommended as a separate material for preperitoneal inguinoplasty.

10.1590/s0102-86502011000800016

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Citations (7)