X. Hua

Medizinisches Versorgungszentrum Prof. Mathey, Prof. Schofer

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Co-Authors

Sonja Schrepfer

Sana Biotechnology (United States)

T. Deuse

Sana Biotechnology (United States)

Mandy Stubbendorff

University Medical Center Hamburg-Eppendorf

Hermann Reichenspurner

University Medical Center Hamburg-Eppendorf

Robert C. Robbins

Harry S. Truman Memorial Veterans' Hospital

H. Reichenspurner

Universität Hamburg

Joachim Velden

Children's Healthcare of Atlanta

R.C. Robbins

Stanford University

H. Reichenspurner

Universität Hamburg

Evangelos D. Michelakis

University of Alberta

Cooperative Institutions

Universität Hamburg

131

University Medical Center Hamburg-Eppendorf

121

German Centre for Cardiovascular Research

Stanford University

Medizinisches Versorgungszentrum Prof. Mathey, Prof. Schofer

University Cancer Center Hamburg

Deutsches Herzzentrum der Charité

Eppendorf (Germany)

Martini-Klinik

Inserm

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Long-term Impact of Postoperative Atrial Fibrillation after Endoscopic Mitral Valve Surgery

The Thoracic and Cardiovascular Surgeon (2025)

Jonas Pausch Jessica Weimann O. D. Bhadra X. Hua Evaldas Girdauskas

10.1055/s-0045-1804196

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Totally Endoscopic Mitral Valve Replacement: Reasons for Non-feasibility of Repair at a High-volume Single Center

The Thoracic and Cardiovascular Surgeon (2025)

F. Sobik P. Jonas O. D. Bhadra X. Hua Evaldas Girdauskas

Single Center

Center (category theory)

Mitral valve replacement

10.1055/s-0045-1804157

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Human Leukocyte Antigen I Knockdown Human Embryonic Stem Cells Induce Host Ignorance and Achieve Prolonged Xenogeneic Survival

Circulation (2011)

T. Deuse Martina Seifert Neil Phillips Andrew Fire Dolly B. Tyan

Although human embryonic stem cells (hESC) have enormous potential for cell replacement therapy of heart failure, immune rejection of hESC derivatives inevitably would occur after transplantation. We therefore aimed to generate a hypoantigeneic hESC line with improved survival characteristics.Using various in vivo, nonischemic, hindlimb xenotransplant models (immunocompetent and defined immunodefective mouse strains) as well as human in vitro T-cell and natural killer (NK)-cell assays, we revealed a central role for T cells in mediating hESC rejection. The NK-cell susceptibility of hESC in vivo was found to be low, and the NK response to hESC challenge in vitro was negligible. To reduce the antigenicity of hESC, we successfully generated human leukocyte antigen (HLA) I knockdown cells (hESC(siRNA+IB)) using both HLA I RNA interference (siRNA) and intrabody (IB) technology. HLA I expression was ≈99% reduced after 7 days and remained low for weeks. Cellular immune recognition of these hESC(siRNA+IB) was strongly reduced in both xenogeneic and allogeneic settings. Immune rejection was profoundly mitigated after hESC(siRNA+IB) transplantation into immunocompetent mice, and even long-term graft survival was achieved in one third of the animals without any immunosuppression. The survival benefit of hESC(siRNA+IB) was further confirmed under ischemic conditions in a left anterior descending coronary artery ligation model.HLA I knockdown hESC(siRNA+IB) provoke T-cell ignorance and experience largely mitigated xenogeneic rejection. By generating hypoantigeneic hESC lines, the generation of acceptable hESC derivatives may become a practical concept and push cell replacement strategies forward.

10.1161/circulationaha.111.020727

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Citations (36)

Heterotopic and Orthotopic Tracheal Transplantation in Mice used as Models to Study the Development of Obliterative Airway Disease

Journal of Visualized Experiments (2010)

X. Hua T. Deuse Karis R. Tang-Quan Robert C. Robbins H. Reichenspurner

Obliterative airway disease (OAD) is the major complication after lung transplantations that limits long term survival (1-7). To study the pathophysiology, treatment and prevention of OAD, different animal models of tracheal transplantation in rodents have been developed (1-7). Here, we use two established models of trachea transplantation, the heterotopic and orthotopic model and demonstrate their advantages and limitations. For the heterotopic model, the donor trachea is wrapped into the greater omentum of the recipient, whereas the donor trachea is anastomosed by end-to-end anastomosis in the orthotopic model. In both models, the development of obliterative lesions histological similar to clinical OAD has been demonstrated (1-7). This video shows how to perform both, the heterotopic as well as the orthotopic tracheal transplantation technique in mice, and compares the time course of OAD development in both models using histology.

Animal model

10.3791/1437-v

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Citations (1)

SCNT-Derived ESCs with Mismatched Mitochondria Trigger an Immune Response in Allogeneic Hosts

Cell stem cell (2014)

T. Deuse Dong Wang Mandy Stubbendorff Ryo Itagaki Antje Grabosch

10.1016/j.stem.2014.11.003

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Citations (55)

563 Identifying the Potassium Channel KCa3.1 as New Therapeutic Target To Prevent Airway Allograft Rejection

The Journal of Heart and Lung Transplantation (2011)

X. Hua T. Deuse Y‐J Chen Heike Wulff Ralf Köhler

10.1016/j.healun.2011.01.575

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Translational models: A humanized model to study the development of intimal hyperplasia after stenting

The Thoracic and Cardiovascular Surgeon (2012)

X. Hua J Boeddinghaus T Gossler Mandy Stubbendorff T. Deuse

Aims: Preclinical in vivo research models to investigate pathobiological and pathophysiological processes in the development of intimal hyperplasia after vessel stenting are crucial for translational approaches. The commonly used animal models include mice, rats, rabbits, and pigs. However, the translation of these models into clinical settings remains difficult, since those biological processes are already studied in animal vessels but never performed before in human research models. We demonstrate a new humanized model to overcome this translational gap.

Intimal hyperplasia

Translational Research

Animal model

10.1055/s-0031-1297524

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Gender-specific Outcome after Off-Pump Coronary Artery Bypass Grafting: A Single-center Retrospective Analysis

The Thoracic and Cardiovascular Surgeon (2025)

F. Sobik X. Hua I. Detlef T. Knochenhauer J. Brickwedel

Single Center

Center (category theory)

10.1055/s-0045-1804021

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Impact of On-Pump Coronary Artery Bypass Graft Surgery vs. Off-Pump on Renal Function in Patients with Impaired Renal Function

The Thoracic and Cardiovascular Surgeon (2024)

F. Sobik X. Hua Shiho Naito I. Detlef Y. Schneeberger

Background: The impact of the surgical technique of coronary artery bypass graft (CABG) surgery on renal function in patients with pre-existing kidney impairment remains a subject of debate. This study aimed to investigate the influence of on-pump (ONCAB) and off-pump (OPCAB) on renal function in patients with pre-existing impaired kidney function (GFR < 60 mL/min).

Impaired renal function

10.1055/s-0044-1780556

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Quantitative and Functional Assessment of Both Ventricles after Ross Procedure versus Conventional Aortic Valve Surgery

The Thoracic and Cardiovascular Surgeon (2024)

X. Hua Christoph Sinning R. Fuhrmann B. Meltem B. Sill

Background: The Ross procedure is well-established for aortic valve diseases (AVD) with excellent long-term outcomes in young and middle-aged patients. However, there is limited knowledge on quantitative and functional changes of left (LV) and right ventricle (RV) after Ross procedure.

10.1055/s-0044-1780682

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