ĽC

Ľ. Chrastinová

National Agricultural and Food Centre

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Andrea Lauková

Slovak Academy of Sciences

M. Chrenková

National Agricultural and Food Centre

Monika Pogány Simonová

Slovak Academy of Sciences

Viola Strompfová

Slovak Academy of Sciences

ĽO

Ľubomír Ondruška

National Agricultural and Food Centre

M. Poláčiková

National Agricultural and Food Centre

Zuzana Formelová

National Agricultural and Food Centre

Renáta Szabóová

University of Veterinary Medicine in Košice

J. Rafay

Animal Production Research Centre

Iveta Plachá

Institute of Animal Physiology of the Slovak Academy of Sciences

Cooperative Institutions

Animal Production Research Centre

Slovak University of Agriculture in Nitra

Slovak Academy of Sciences

National Agricultural and Food Centre

Institute of Animal Physiology of the Slovak Academy of Sciences

University of Veterinary Medicine in Košice

Bioscience (Slovakia)

Palacký University Olomouc

Ocní klinika

University Hospital Olomouc

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Utilization of an extract product from ginseng supplementation in diets and different energy levels of granulated feed in the nutrition of rabbits.

Archiva zootechnica (2009)

Ľ. Chrastinová M. Chrenková M. Poláčiková Andrea Lauková Monika Pogány Simonová

In fattening experiments a total of 96 th day of age, Hycole hybrid) were fed a diets containing 15.2 % (groups A,B) or 41 % (group C) of dehydrated lucerne meal in combination with 67 % or 39% of agricultural byproducts and rape oil (1.7 % vs. 0 %) in group. In one experimental group (EG) we studied the effect of Eleutherococcus senticosus dry extract which was applied to feed mixture at 30g/100kg on the zootechnical, microbiological and biochemical parameters in rabbits as well as quality of rabbit meat. Treated animals were compared with untreated ones (control diet B). Rabbits were fed three diets ad libitum. The study was conducted to evaluate chemical composition of meat from rabbits slaughtered at 77 day of age. The MLD (Musculus longissimus dorsi) muscles were sampled from nine animals. Fatty acids in intramuscular fat are composed of 42.1, 40.2, 31.5% monounsaturated (MUFA), 17.9, 18.2, 20.8 % to polyunsaturated (PUFA) and 39.7, 41.9, 47.7 % saturated fatty acids (SFA). The ratio PUFA/SFA (0.43 vs. 0.46), the atherogenic index (0.63 vs. 0.91), and the ratio n-6/n-3 (7.59 vs. 6.23) were calculated. Slaughter parameters and the quality of meat were practically the similar in each experimental group. All animals were found in good health conditions during the trial. The application of Eleutherococcus reduced the mortality and increased feed conversion ratio and average daily weight gain (P<0.05).

Intramuscular fat

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Citations (4)

Experimental Application of Sage in Rabbit Husbandry

Acta Veterinaria Brno (2008)

Renáta Szabóová Andrea Lauková Ľ. Chrastinová Monika Pogány Simonová Viola Strompfová

Salvia spp. belongs to the Labiatae family and is characterized by antimicrobial and antiinflammatory effect. The aim of this study was to test its in vitro and in vivo inhibitory effect against bacteria as well as to find an alternative possibility to use sage in the rabbit ecosystem examining biochemical, zootechnical and inmunological indicators, compared to the commercial feed mixture Xtract. Using the sage extract in in vitro tests, its inhibitory effect was noted. Under in vivo conditions, in the experimental group with sage (EG1), reduction of Pseudomonas -like sp. ( p < 0.01) and E. coli ( p < 0.01) was noted after 7 days of sage application compared to the control group CG2 (with Robenidin) as well as after 21 days of sage extract application, when the reduction of coagulase-negative staphylococci ( p < 0.01) was detected (in comparison with the experimental group-EG2, Xtract group). In the caecum of rabbits from EG1, higher values of lactic, acetic and butyric acids were noted. The values of propionic acid were not influenced. Biochemical indicators were not influenced; however, the values of GSH Px were lower in EG1 compared to EG2. Higher phagocytic activity (18%) was noted in EG1 than in EG2 (13%) after 21 days of additives application. The reduction of Eimeria sp. oocysts was demonstrated in EG1 (sage group) after 7 days of sage application comparing to CG2 (217 OPG to 566 OPG). The animals in both experimental groups achieved higher feed consumption and weight gain, lower mortality compared to both controls. Neither of the additives had a negative influence on the health status and growth performance of rabbits.

Caecum

10.2754/avb200877040581

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Citations (23)

Quality of rabbit meat after application of bacteriocinogenic and probiotic strain Enterococcus faecium CCM 4231 in rabbits.

International Journal of Probiotics and Prebiotics (2009)

Monika Pogány Simonová Renáta Szabóová M. Haviarová Ľ. Chrastinová J. Mojto

Enterococcus faecium

Rabbit (cipher)

Strain (injury)

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Citations (10)

[Effect of the number of transferred embryos on multiple pregnancy].

PubMed (1999)

Jana Březinová M Svobodová M Tálas Ľ. Chrastinová Jiřı́ Dostál

The aim of this retrospective study was to assess the influence of the number of transferred embryos on multiple pregnancy.Retrospective clinical study.Department of Obstetric and Gynecology FN UP, Olomouc.Over the last seven years 572 embryotransfers (ET) have been performed in our centre.Of the 173 pregnancies (P) achieved there were 56% singletons, 35% twins and 9% triplets. One embryo was transferred in 10% of cases and all 8 pregnancies (15% P/ET) ended with a live birth. Two embryos were transferred in 17% of cases. The pregnancy rate was 20% P/ET, 55% of pregnancies were twins and 10% pregnancies were lost in abortion or ectopic pregnancy. Three embryos were transferred in most cases (62%). The pregnancy rate was the highest in this group (36% P/ET) with 13% twins and 3% triplets. The overall pregnancy loss was 16%, the highest loss, 27%, recorded in triplets. Four embryos were transferred in 11% of cases. The pregnancy rate decreased to 28% P/ET, as well as the number of twins (6%), while the number of triplets increased slightly (5%). Pregnancy loss occurred only in singleton pregnancies.We can conclude that in our setting the pregnancy rate increases with a higher number of embryos up to three embryos transferred. Unfortunately, the higher pregnancy rate with three embryos transferred is on the expense of a higher risk and losses due to multiple pregnancy. Therefore we recommend to transfer two embryos unless there are some specific reasons.

Singleton

Twin Pregnancy

Live birth

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THE EFFECT OF NATURAL ZEOLITE DIETARY SUPPLEMENTATION ON THE EGG QUALITY OF JAPANESE QUAILS (Coturnix japonica) DURING THE LAYING PERIOD

Journal of Microbiology Biotechnology and Food Sciences (2021)

E. Hanusová Cyril Hrnčár M. Oravcová Ľ. Chrastinová M. Rajský

The effect of supplementing natural zeolite (NZ) on the laying characteristics and on the internal and external egg quality during laying period of Japanese quails was studied. The laying and average eggs per female were lower in control (C) and the Z1 experimental groups (1% NZ) compare with the Z2 experimental group (2% NZ). The significant effect of the NZ addition in comparison with the C was found in the eggshell weight and yolk index. The eggshell weight of C (1.10±0.01 g) was significantly lower than Z1 (1.18±0.01 g). The quails of Z2 had significantly lower egg weight than Z1. The average egg weight was significantly affected by diet (P≤0.05) and also by age of quails (P≤0.001). At the beginning of the laying, the weight of the eggs (age 1) was significantly lower (12.07±0.12 g at the beginning, 12.38±0.12 g in the middle and 13.38±0.12 g in the end) (P≤0.001). The albumen weight and Haugh unit were significantly influenced by the age of the females. The albumen weight increased with female age (from 6.88 to 8.10±0.09 g). The HU was significantly lower (89.52±0.30) at the age 1 than in age 2 (90.50±0.30) and age 3 (90.57±0.30) (P≤0.05). The yolk index was significantly affected by both diet and age of animals. The significant difference was between C and Z1 (P≤0.05). The highest yolk index was found in youngest female (P≤0.01). The egg quality was mainly affected by age of females. Diet affected investigated traits to a smaller extent.

Yolk

Coturnix coturnix

Haugh unit

Laying

10.15414/jmbfs.4631

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THE EFFECT OF YUCCA SCHIDIGERA EXTRACT IN DIET OF RABBITS ON NUTRIENT DIGESTIBILITY AND QUALITATIVE PARAMETERS IN CAECUM

Slovak Journal of Animal Science (2012)

M. Chrenková Ľ. Chrastinová M. Poláčiková Zuzana Formelová Andrej Baláži

The aim of this study was to determine the effects of yucca (Yucca schidigera) powder extract added to the rabbit feed mixtures. The animals of experimental group (EG1) were fed diet enriched with supplement of Yucca schidigera dry extract 5g/100 kg feed; rabbits of EG2 had diet enriched with yucca dry extract 20g/100 kg feed for 42 days. The control group (CG) was fed untreated pellet diet. The experiment was carried out on commercial hybrid Hycole rabbits. Between 65 and 70 days of age, 3 male rabbits from each group were selected for digestibility tests using the balance method. The faeces were collected individually during 5 consecutive days according to the European reference method for rabbit digestion trials. Chemical analysis of the diets and faeces for dry matter, crude protein, crude fibre, crude fat, nitrogen free extract, organic matter and starch was carried out according to the same method. The caecal samples from each of three slaughtered rabbits were collected for analysis; pH, VFA, ammonia-N and lactic acid were determined. The rabbits in our experiment showed the lowest coefficients of digestibility of crude fibre, and higher digestibility of crude protein, crude fat and organic matter, but the differences were not statistically significant comparing with control diet. The effect of yucca extract addition is manifested in reducing ammonia levels in the caecum of rabbits.

Caecum

Digestion

Completely randomized design

Yucca

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Citations (17)

The effect of dietary supplementation of sage plant extract and Enterocin M on the mucus in the the small intestine and caecum in rabbits

Polish Journal of Veterinary Sciences (2023)

Renáta Szabóová Andrea Lauková R. Herich Lucia Tarabová Ľ. Chrastinová

The aim of this study was to determine the beneficial effect of natural substances - enterocin M (Ent M; the proteinaceous substance produced by Enterococcus faecium CCM8558) and sage plant ( Salvia officinalis L.) extract on the production of mucus in the rabbits small intestine and caecum. Sixty four post-weaned rabbits (meat line M91) were divided into three experimental groups (EG - Ent M; SG - sage extract; ESG - combination Ent M with sage extract) and control group (CG). The experiment lasted for 35 days, the natural substances were administered during the first 21 days, Ent M in EG/ESG, sage extract in SG/ESG. The beneficial effect on mucus production quantity occured in the duodenum (p⟨0.001) and jejunum (p⟨0.01) in ESG compared to that found in CG on day 21, the prolonged effect in EG in the duodenum (p⟨0.001) compared to that observed in CG at the end of the experiment and to that in EG on day 21. The novelty of the study is in the application and monitoring the effect of non-rabbit-derived probiotic strain ( Enterococcus faecium CCM8558) bacteriocin - Enterocin M and sage plant extract on mucus quantity (expressed in gram) in different segments of the rabbit small intestine as well as the caecum. The results obtained indicate that supplementation of selected natural substances in the feed has the potent stimulatory effects on mucus production in the rabbit small intestine.

Caecum

Enterococcus faecium

Jejunum

Salvia officinalis

10.24425/pjvs.2020.135815

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Citations (0)

P-050. Histochemistry and ultrastructure of the endometrium after hormonal stimulation

Human Reproduction (1997)

Jiřı́ Dostál I Oborná Radko Novotný J Malínský V Lichnovský

10.1093/humrep/12.suppl_2.143-a

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Combined administration of bacteriocin- -producing, probiotic strain Enterococcus faecium CCM7420 with Eleutherococcus senticosus and their effect in rabbits

Polish Journal of Veterinary Sciences (2013)

Monika Pogány Simonová A Laukovtá Ľ. Chrastinová Iveta Plachá Viola Strompfová

Abstract The effect of Enterococcus faecium CCM7420 (EF) - enterocin-producing and probiotic strain of rabbit origin, Eleutherococcus senticosus extract (ES) and their combination (ES+EF) was determined on selected bacteria in faeces and caecum content, leukocytes phagocytosis, blood biochemistry and growth performance. Ninety-six weaned rabbits were divided into 3 experimental (ES, EF, ES+EF) and control group (CG). The rabbits in the groups ES and EF+ES were fed commercial diet enriched with E. senticosus extract (30 g/100 kg feed), rabbits in groups EF and CG were fed untreated diet. The rabbits in the EF and ES+EF groups were administered with an overnight culture of E. faecium CCM7420 strain (500 μl/animal/day into water, 109 CFU/ml). The treatment period lasted 21 days. The microbiological examinations in faecal samples confirmed the presence of E. faecium CCM7420 strain. In groups EF and ES+EF, the reduction of faecal coliforms, Pseudomonas-like sp., Clostridium-like sp. and S. aureus was recorded. Leucocyte phagocytosis significantly increased in all experimental groups (P<0.0001) compared to CG. The lowest GPx values were measured in the ES+EF group. Higher total protein, triglycerides and calcium concentrations were detected in experimental groups compared to CG. The cholesterol concentration decreased in the ES group. The highest average daily gain was recorded in EF group; in ES+EF the better feed conversion ratio and no mortality was recorded. These results indicated that the dietary supplementation with the E. faecium CCM7420 and E. senticosus extract stimulate the leukocytes phagocytosis and reduces the potential pathogens in rabbits digestive tract without oxidative stress and improve the growth performance.

Enterococcus faecium

Caecum

10.2478/pjvs-2013-0089

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Citations (15)

Enterococcus faecium CCM7420, bacteriocin PPB CCM7420 and their effect in the digestive tract of rabbits

Czech Journal of Animal Science (2009)

Monika Pogány Simonová Andrea Lauková Ľ. Chrastinová Viola Strompfová Š. Faix

The effect of Enterococcus faecium CCM7420, bacteriocin-producing strain with probiotic properties and its partially purified bacteriocin PPB CCM7420 on growth performance, microflora, Eimeria sp. oocysts, biochemical blood parameters and glutathione-peroxidase activity in rabbits was determined. An increase in the body weight of rabbits (P < 0.01) was achieved after E. faecium CCM7420 application. A non-significant reduction of faecal E. coli (including haemolytic E. coli), coagulase-positive staphylococci (CPS) and Staphylococcus aureus was found in rabbits administered the E. faecium CCM7420 strain and lower (non-significant) counts of S. aureus and Clostridium-like sp. were detected in PPB CCM7420 group, compared to the control. In the caecum, a significant reduction of CPS was noted in both experimental groups (P < 0.001 and P < 0.05 for EG1 and EG2, respectively). Biochemical blood parameters increased in both experimental groups (P < 0.05 and P < 0.001 for EG2 and EG1, respectively). In the CCM7420 group, the lowest activity of glutathione-peroxidase was measured (P < 0.001). After the application of PPB CCM7420 (P < 0.05; day 21), a reduction of Eimeria sp. oocysts was recorded.

Enterococcus faecium

Caecum

Clostridium perfringens

Enterococcus faecalis

10.17221/1659-cjas

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Citations (65)