Marcial Llanillo

Universidad de Salamanca

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Ángel Hernández‐Hernández

Instituto de Investigación Biomédica de Salamanca

Jesús Sánchez‐Yagüe

Instituto de Investigación Biomédica de Salamanca

Guillermo López-Ruano

Universidad de Salamanca

Rodrigo Prieto-Bermejo

Universidad de Salamanca

Fermín Sánchez‐Guijo

Instituto de Investigación Biomédica de Salamanca

Atanasio Pandiella

Centro de Investigación Biomédica en Red de Cáncer

Sara Gutiérrez-Herrero

Instituto de Investigación Biomédica de Salamanca

Carmen Guerrero

Instituto de Investigación Biomédica de Salamanca

Marta Romo-González

Rutgers, The State University of New Jersey

Consuelo del Cañizo

Instituto de Investigación Biomédica de Salamanca

Cooperative Institutions

Universidad de Salamanca

Instituto de Investigación Biomédica de Salamanca

Consejo Superior de Investigaciones Científicas

Centro de Investigación del Cáncer

Centro de Investigación Biomédica en Red de Cáncer

Complejo Hospitalario de Salamanca

Utrecht University

Universitat de Barcelona

Hospital Universitario Virgen del Rocío

Instituto de Salud Carlos III

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Supplementary Figure 8 from NADPH Oxidases as Therapeutic Targets in Chronic Myelogenous Leukemia

Beatriz Sánchez-Sánchez Sara Gutiérrez-Herrero Guillermo López-Ruano Rodrigo Prieto-Bermejo Marta Romo-González

PDF file - 87KB, Supplementary Figure 8. Inhibitors of NADPH oxidases and Flt3-ITD synergize to prevent the proliferation of two different AML cell lines.

Chronic myelogenous leukemia

10.1158/1078-0432.22452114

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PTPN13 and β-Catenin Regulate the Quiescence of Hematopoietic Stem Cells and Their Interaction with the Bone Marrow Niche

Stem Cell Reports (2015)

Guillermo López-Ruano Rodrigo Prieto-Bermejo Teresa Lopes Ramos Laura San‐Segundo Luis Ignacio Sánchez‐Abarca

The regulation of hematopoietic stem cells (HSCs) depends on the integration of the multiple signals received from the bone marrow niche. We show the relevance of the protein tyrosine phosphatase PTPN13 and β-catenin as intracellular signaling molecules to control HSCs adhesiveness, cell cycling, and quiescence. Lethally irradiated mice transplanted with Lin– bone marrow cells in which PTPN13 or β-catenin had been silenced showed a significant increase of long-term (LT) and short-term (ST) HSCs. A decrease in cycling cells was also found, together with an increase in quiescence. The decreased expression of PTPN13 or β-catenin was linked to the upregulation of several genes coding for integrins and several cadherins, explaining the higher cell adhesiveness. Our data are consistent with the notion that the levels of PTPN13 and β-catenin must be strictly regulated by extracellular signaling to regulate HSC attachment to the niche and the balance between proliferation and quiescence.

Hematopoietic stem cell

10.1016/j.stemcr.2015.08.003

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Citations (15)

Platelet linoleic acid is a potential biomarker of advanced non-small cell lung cancer

Experimental and Molecular Pathology (2009)

Javier de Castro Marina Rodríguez Vicenta S. Martínez‐Zorzano Marcial Llanillo Jesús Sánchez‐Yagüe

10.1016/j.yexmp.2009.08.002

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Citations (22)

Changes in forebrain, cerebellum and brain stem phospholipid patterns from mothers and newborn rats after chronic pentazocine treatment during the gestation period.

PubMed (1988)

Raquel de Andrés J.A. Cabezas Marcial Llanillo

Pregnant female Wistar rats were injected with pentazocine intramuscularly during the first nineteen days of the gestation period. The initial dose (1.8 mg/kg body weight) was increased daily by 1.2 mg of pentazocine, finishing with 23.4 mg/kg body weight. Chemical analysis of the forebrain, cerebellum and brain stem from mothers and their newborns points to statistically non-significant changes in total protein, phospholipid and cholesterol contents after pentazocine treatment. The phospholipid pattern only changed significantly in the brain stem of the mother rats treated with pentazocine, showing an increase (p less than 0.05) in inositol phosphoglycerides (2.5-fold) and phosphatidylglycerol (3.5-fold) percentages, while ethanolamine- and serine-phosphoglycerides decreased (p less than 0.05) by about 0.9 and 0.6-fold, respectively. The cholesterol profile found in the forebrains of the mother rats and their newborns did not show statistically significant differences after treatment.

Pentazocine

Forebrain

Gestation period

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Citations (3)

ISOLATION AND COMPOSITION OF THE MEMBRANE FROM SHEEP PLATELETS

Biochemical Society Transactions (1981)

Marcial Llanillo José A. Cabezas

Conference Abstract| April 01 1981 ISOLATION AND COMPOSITION OF THE MEMBRANE FROM SHEEP PLATELETS Marcial Llanillo; Marcial Llanillo 1Dep. Biochemistry, Fac. Biology & Pharmacy, Univ. Salamanca and C. S. I. C., Salamanca, Spain Search for other works by this author on: This Site PubMed Google Scholar José A. Cabezas José A. Cabezas 1Dep. Biochemistry, Fac. Biology & Pharmacy, Univ. Salamanca and C. S. I. C., Salamanca, Spain Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1981) 9 (2): 143P. https://doi.org/10.1042/bst009143p Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Cite Icon Cite Get Permissions Citation Marcial Llanillo, José A. Cabezas; ISOLATION AND COMPOSITION OF THE MEMBRANE FROM SHEEP PLATELETS. Biochem Soc Trans 1 April 1981; 9 (2): 143P. doi: https://doi.org/10.1042/bst009143p Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1981 Biochemical Society1981 Article PDF first page preview Close Modal You do not currently have access to this content.

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10.1042/bst009143p

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Supplementary Figure 3 from NADPH Oxidases as Therapeutic Targets in Chronic Myelogenous Leukemia

Beatriz Sánchez-Sánchez Sara Gutiérrez-Herrero Guillermo López-Ruano Rodrigo Prieto-Bermejo Marta Romo-González

PDF file - 44KB, Supplementary figure 3. Antioxidants and rotenone treatment hamper K562 cell proliferation. DPI treatment reduces BCR-ABL protein levels.

Chronic myelogenous leukemia

K562 cells

breakpoint cluster region

10.1158/1078-0432.22452132

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Supplementary Figure 5 from NADPH Oxidases as Therapeutic Targets in Chronic Myelogenous Leukemia

Beatriz Sánchez-Sánchez Sara Gutiérrez-Herrero Guillermo López-Ruano Rodrigo Prieto-Bermejo Marta Romo-González

PDF file - 340KB, Supplementary figure 5. Combination of NADPH oxidases and BCR-ABL inhibitors progressively increases the proportion of sub-G1 phase cells.

Chronic myelogenous leukemia

breakpoint cluster region

10.1158/1078-0432.22452123

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Amphiphilic and hydrophilic nature of sheep and human platelet phosphotyrosine phosphatase forms

Biochimica et Biophysica Acta (BBA) - Biomembranes (1999)

Ángel Hernández‐Hernández Marcial Llanillo M. C. Rodríguez Francisco Pedro Gómez Gómez Jesús Sánchez‐Yagüe

10.1016/s0005-2736(99)00066-8

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Citations (14)

Loss of phosphotyrosine phosphatase activity and changes in the tyrosine phosphorylation state of proteins after storage of sheep platelets in plasma or Seto solution at 4 °C

Vox Sanguinis (2001)

Ángel Hernández‐Hernández Carmen Sánchez‐Bernal M.C. Rodríguez Francisco Pedro Gómez Gómez Marcial Llanillo

Background and Objectives During platelet storage an array of deleterious changes occur, through mechanisms not fully understood, which impair platelet haemostasis. Transfused platelets should maintain the integrated networks of signalling pathways that regulate platelet activation and functionality. We hypothesized that protein phosphorylation and dephosphorylation, which play a fundamental role in these pathways, might be affected by platelet storage. We therefore investigated whether the activity of phosphotyrosine phosphatase (PTP), which belongs to an oxidant‐susceptible group of enzymes involved in the platelet signal‐transduction pathways that ensure platelet functionality, is affected by platelet storage. Materials and Methods Using sheep platelet species as a model system, we conducted serial studies on the membranes of platelets and microparticles shed during platelet storage, in their own plasma or in a synthetic medium called Seto, for up to 5 days at 4 °C. Results A progressive decrease in both total and specific membrane‐associated PTP activities from whole platelets (but not from microparticles) located within each platelet storage bag was observed from day 1 onwards in both types of storage media. These decreases could be partly avoided by the addition of vitamin E. Additionally, the observed decrease in PTP activity was accompanied with increases in the tyrosine phosphorylation of proteins from whole platelets or crude platelet membranes, the tyrosine phosphorylation state of proteins from microparticles remaining basically unchanged. Conclusion Our findings suggest that alterations of at least the tyrosine phosphorylation balance might be one of the reasons for the decrease in the haemostatic function of stored platelets.

10.1046/j.1423-0410.2001.00111.x

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Citations (4)

Fatty acid composition of subcellular particles from sheep platelets and topological distribution of phosphatidylethanolamine fatty acids in the plasma membrane

Lipids (1991)

Jesús Sánchez‐Yagüe José A. Cabezas Marcial Llanillo

Abstract The fatty acid composition of individual phospholipids in subcellular fractions of sheep platelets and the asymmetrical distribution of phosphatidylethanolamine (PE) fatty acyl chains across the plasma membrane were examined. The main fatty acids of total lipid extracts were oleic (18∶1; 32–41%), linoleic (18∶2, 10–17%), stearic (18∶0; 13–15%), palmitic (16∶0; 11–15%) and arachidonic (20∶4; 8–12%) acids, with a saturated/unsaturated ratio of about 0.4. Each phospholipid class had a distinct fatty acid pattern. Sphingomyelin (SM) showed the highest degree of saturation (50%), with large proportions of behenic (22∶0), 18∶0 and 16∶0 acids. The main fatty acid in PE, phosphatidylserine (PS) and phosphatidylcholine (PC) was 18∶1n−9. Our findings suggest that fatty acids are asymmetrically distributed between thecholine versus the non‐choline phospholipids, and also between plasma membranes and intracellular membranes. The transbilayer distribution of PE fatty acids in plasma membranes from non‐stimulated sheep platelets was investigated using trinitrobenzenesulfonic acid (TNBS). A significant degree of asymmetry was found, which is a new observation in a non‐polar cell. The PE molecules from the inner monolayer contained higher amounts of 18∶2 and significantly less 18∶1 and 20∶5 than those found in the outer monolayer, although no major differences were detected in the transbilayer distribution of total unsaturated versus saturated PE acyl chains.

Phosphatidylethanolamine

Stearic acid

10.1007/bf02535971

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Citations (4)