Hai‐Ying Jin

Zhejiang Chinese Medical University

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Document Type

Co-Authors

Kowichi Jimbow

Sapporo Medical University

Toshiharu Yamashita

Sapporo Medical University

Xiaomei Ye

Jiangsu Academy of Agricultural Sciences

Dan-Lu Song

Annoroad Gene Technology (China)

Meisen Shi

China University of Political Science and Law

Jia-Ping Lu

Jinzhong University

Qingsheng Qi

Shandong University

Kuninori Hirosaki

National Hospital Organization Hokkaido Medical Center

C. Han

Fudan University

Qian Wang

Shandong University

Cooperative Institutions

Capital Medical University

Sapporo Medical University

Fudan University

Shandong University

Shanghai Jiao Tong University

Sun Yat-sen University

Guiyang Medical University

Chinese Academy of Sciences

Chinese Academy of Medical Sciences & Peking Union Medical College

State Key Laboratory of Microbial Technology

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[Establishment and Verification of 6-color Fluorescent-labeled Rapid PCR Amplification System].

PubMed (2016)

Yaju Liu Juntao Zhang Hai‐Ying Jin Meisen Shi

To establish the rapid PCR amplification program and system and to verify the technical indexes.PCR multiplex and capillary electrophoresis detection of 24 autosomal STR loci and one Y-STR loci using the 6-color fluorescence marking technology, as well as A melogenin and Y-InDel. Meanwhile, sensitivity, specificity, identity, stability, mixing and a batch of sample tests were investigated, and the genotype of various routine samples and degraded, exfoliated cell samples were observed.The sensitivity of the system was 0.062 5 ng. In addition, the genotype could be detected accurately only around 65 min via rapid amplification. The species-specificity was high and the genotyping of all kinds of dry blood specimens of filter paper and mixed, degraded, exfoliated cell samples were accurate.The rapid amplification system can significantly improve the detection rate, and obtain accurate and stable genotyping results, which may be important implications for the establishment of STR database and study on population genetics and forensic identification.

Indel

Multiplex

Source

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Citations (0)

Cardiac Computed Tomography to Guide the Selection of Better Display Angle of Cardiac Right Ventricular Pacing Electrode in Radiography

Current Medical Imaging Formerly Current Medical Imaging Reviews (2023)

Zhan Hong Wang HongGuang Miao Wei Qin Wang SiYao Kang Hai‐Ying Jin

Traditional radiography angles do not adequately reveal the shape and position of the right ventricular pacing electrode.This study aimed to explore better radiography angles with the help of cardiac computed tomography (CT).We analyzed the cardiac CT images of consecutive outpatients from 2018 to 2019. The right anterior oblique (RAO) 30° and the left anterior oblique (LAO) 40° were found to sufficiently display the shape and position of the right ventricular pacing electrode.A total of 214 consecutive outpatients were enrolled, whose average age was 55.0 ± 13.0 years, and 151 were male (70.6%). Through analyzing the cardiac CT images, the α angle (33.7° ± 6.1) and the γ angle (38.8° ± 8.0) were determined. Furthermore, we verified these angles in 48 patients after pacemaker implantation. The results showed that the ratio of the length of right ventricular electrode using the RAO α angle (≈30°) to the posterior-anterior position (PA position) was 1.099 ± 0.157 vs. 1.053 ± 0.182 (the ratio using the traditional RAO 45°) (P < 0.001). We observed that the relationship between the right ventricular active electrode and the ventricular septum was better identified using the LAO γ angle (≈40°) than the traditional 60° angle.With the help of cardiac CT, we found that RAO 30° could better show the shape and length of the right ventricular pacing electrode, and LAO 40° could better show the positional relationship between the pacing electrode and the ventricular septum.

Ventricular pacing

Position (finance)

Cardiac Pacing

Lead (geology)

10.2174/1573405619666230216092351

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Intracellular Vesicular Trafficking of Tyrosinase Gene Family Protein in Eu‐ and Pheomelanosome Biogenesis

Pigment Cell Research (2000)

Kowichi Jimbow CHEN HUA Paul Gomez Kuninori Hirosaki Kyoka Shinoda

The intracellular vesicular trafficking in the melanosome biogenesis (melanogenesis) is reviewed with the incorporation of our own experimental findings. The melanosome biogenesis involves four stages of melanosome maturation, which reflect the transport of structural and enzymatic proteins from Golgi (trans‐Golgi network: TGN) to the melanosomal compartment and their organization therein. The major melanosomal proteins include tyrosinase gene family protein (tyrosinase and tyrosinase‐related protein; TRP), lysosome‐associated membrane protein (Lamp) and gp100 (pmel 17). They are glycosylated in the endoplasmic reticulum, and transported by vesicles from the TGN to the melanosomal compartment. During the formation of transport vesicles, they assemble on the cytoplasmic face of the TGN to select cargo by interacting directly or indirectly with coat proteins. Tyrosinase and TRP‐1 possess the dileucine motifs at the cytoplasmic domain, to which adapter protein‐3 binds to transport them from the TGN to stage I melanosomes (related to late endosomes) and then to stage II melanosomes. A number of small guanosine triphosphate‐binding proteins, including rab 7, appear to be involved in this vesicular transport. Phosphatidyl inositol 3 kinase also regulates this membrane trafficking of melanosomal glycoprotein. Eumelanogenesis is controlled by melanocyte‐stimulating hormone, and all three tyrosinase gene family proteins are transported from the TGN to stage II melanosomes that are elliposoidal and contain the structural matrix of filaments/lamellae. In contrast, pheomelanogenesis is primarily regulated by agouti signal protein, and only tyrosinase is transported from stage I melanosomes to stage II melanosomes that are spherical and related to lysosomes. Because of the absence of TRP‐1 and TRP‐2 in pheomelanogenesis, it may be suggested that tyrosinase is involved in lysosomal degradation after forming dopaquinone, to which the cysteine present in the lysosomal granule binds to form cysteinyldopas that will then be auto‐oxidized to become pheomelanin.

Melanosome

Transport protein

Vesicular Transport Proteins

ER retention

10.1034/j.1600-0749.13.s8.20.x

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Citations (54)

Local administration of hepatocyte growth factor gene enhances the regeneration of dermis in acute incisional wounds

Journal of Surgical Research (2004)

Ichiro Ono Toshiharu Yamashita Tokimasa Hida Hai‐Ying Jin Yoshinori Ito

10.1016/j.jss.2003.08.242

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Citations (67)

Detection of tyrosinase and tyrosinase-related protein 1 sequences from peripheral blood of melanoma patients using reverse transcription-polymerase chain reaction

Journal of Dermatological Science (2003)

Hai‐Ying Jin Toshiharu Yamashita Yasushi Minamitsuji Fusayuki Omori Kowichi Jimbow

Melanocyte

10.1016/j.jdermsci.2003.09.003

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Citations (14)

A CRISPR-Cas9 Assisted Non-Homologous End-Joining Strategy for One-step Engineering of Bacterial Genome

Scientific Reports (2016)

Tianyuan Su Fapeng Liu Pengfei Gu Hai‐Ying Jin Yizhao Chang

Abstract Homologous recombination-mediated genome engineering has been broadly applied in prokaryotes with high efficiency and accuracy. However, this method is limited in realizing larger-scale genome editing with numerous genes or large DNA fragments because of the relatively complicated procedure for DNA editing template construction. Here, we describe a CRISPR-Cas9 assisted non-homologous end-joining (CA-NHEJ) strategy for the rapid and efficient inactivation of bacterial gene (s) in a homologous recombination-independent manner and without the use of selective marker. Our study show that CA-NHEJ can be used to delete large chromosomal DNA fragments in a single step that does not require homologous DNA template. It is thus a novel and powerful tool for bacterial genomes reducing and possesses the potential for accelerating the genome evolution.

Non-homologous end joining

Genome Engineering

Bacterial genome size

10.1038/srep37895

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Citations (121)

Developmental Validation of a novel 5 dye Y-STR System comprising the 27 YfilerPlus loci

Scientific Reports (2016)

Rufeng Bai Yaju Liu Zheng Li Hai‐Ying Jin Qinghua Tian

Abstract In this study, a new STRtyper-27 system, including the same Yfiler Plus loci (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a/b, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, Y-GATA H4, DYS449, DYS460, DYS481, DYS518, DYS533, DYS570, DYS576, DYS627 and DYF387S1a/b), was established using a set of 5 fluorescent dye labels. Primers, internal size standard, allelic ladders and matrix standard set were designed and created in-house for this multiplex system. This paper describes the validation studies conducted with the STRtyper-27Y system using a 3130XL genetic analyzer for fragment length detection that included the analysis of the following parameters and aspects: sensitivity, species specificity, inhibition, haplotype concordance, precision, stutter, DNA mixtures, and stability studies with crime scene samples. The studies demonstrated, that the STRtyper-27Y system provided equivalent overall performance comparable to the latest Yfiler Plus kit, but with enhanced compatibility in terms of instrument platforms and software allowing forensic laboratories to conduct its forensic application and evaluate its performance, all in their own 5 dye Y-STR chemistry system /environment without software or instrument upgrades.

Multiplex

STR analysis

Concordance

Y-STR

10.1038/srep29557

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Citations (18)

MicroRNA-495 inhibits the high glucose-induced inflammation, differentiation and extracellular matrix accumulation of cardiac fibroblasts through downregulation of NOD1

Cellular & Molecular Biology Letters (2018)

Xiaowei Wang Hai‐Ying Jin Shifeng Jiang Yanlan Xu

MicroRNAs (miRNAs) have physiological and pathophysiological functions that are involved in the regulation of cardiac fibrosis. This study aimed to investigate the effects of miR-495 on high glucose-induced cardiac fibrosis in human cardiac fibroblasts (CFs) and to establish the mechanism underlying these effects.Human CFs were transfected with an miR-495 inhibitor or mimic and incubated with high glucose. The levels of NOD1 and miR-495 were then determined via quantitative RT-PCR. Pro-inflammatory cytokine levels, cell differentiation and extracellular matrix accumulation were respectively detected using ELISA, quantitative RT-PCR and western blot assays. The luciferase reporter assay, quantitative RT-PCR and western blot were used to explore whether NOD1 was a target of miR-495. The effects of miR-495 on the NF-κB and TGF-β1/Smad signaling pathways were also detected via western blot.Our results show that high glucose can significantly increase the expression of NOD1 in a time-dependent manner. Upregulation of miR-495 significantly alleviated the high glucose-induced increases in cell differentiation and collagen accumulation of CFs. Moreover, the bioinformatics analysis predicted that NOD1 was a potential target gene for miR-495. The luciferase reporter assay showed that miR-495 can directly target NOD1. The introduction of miR-495 could significantly inhibit the high glucose-activated NF-κB and TGF-β1/Smad signaling pathways.Upregulation of miR-495 ameliorates the high glucose-induced inflammatory, cell differentiation and extracellular matrix accumulation of human CFs by modulating both the NF-κB and TGF-β1/Smad signaling pathways through downregulation of NOD1 expression. These results provide further evidence for the protective effect of miR-495 overexpression in cases of high glucose-induced cardiac fibrosis.

Cardiac Fibrosis

10.1186/s11658-018-0089-x

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Citations (35)

Developmental Validation of the Novel Five-Dye-Labeled Multiplex Autosomal STR Panel and Its Forensic Efficiency Evaluation

Frontiers in Genetics (2022)

Shimei Huang Xiaoye Jin Hongling Zhang Hai‐Ying Jin Zheng Ren

Short tandem repeats (STRs) are the most frequently used genetic markers in forensic genetics due to their high genetic diversities and abundant distributions in the human genome. Currently, the combined DNA index system is commonly incorporated into various commercial kits for forensic research. Some novel STRs that are different from the combined DNA index system were not only used to assess complex paternity cases but also could provide more genetic information and higher forensic efficiency in combination with those commonly used STRs. In this study, we validated forensic performance of a novel multiplex amplification STR panel to evaluate its sensitivity, species specificity, forensic application values, and so on. Obtained results revealed that the kit showed high sensitivity, and the complete allelic profile could be observed at 0.125 ng DNA sample. In addition, the kit possessed high species specificity, good tolerance to common inhibitors, and accurate genotyping ability. More importantly, STRs out of the kit displayed high discrimination power and probability of exclusion. To sum up, the novel kit presented in this study can be viewed as a promising tool for forensic human identification and complex paternity analysis.

Multiplex

STR multiplex system

STR analysis

DNA profiling

Forensic identification

10.3389/fgene.2022.897650

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Citations (4)

Clinical evaluation and design of network live broadcast system of DSA video streaming

Zhonghua fangshexian yixue zazhi (2008)

Bin-jie Wang Heping Zhang Hai‐Ying Jin

Objective To evaluate the clinical feasibility of network live broadcast of digital subtraction angiography(DSA)video streaming.Methods DSA video streaming was captured by an advanced image capture board.MPEG-4 and Directshow framework were used for data compression and transmission.Data of DSA video streaming could be transmitted easily from server sender filter to client receiver filter according to TCP and UDP protocols.Images of 24 cases were captured,which were compared with images of DSA workstation by experienced doctors.The subiective evaluation criteria included the manifestition of normal and pathological blood vessels,and sharpness,contrast degree and real time efficiency of images.Results The delay time of live broadcast was less than one second in 100 M LAN.Among 24 cases,excellent imaging quality was got in 17 cases,good in 5 cases and midst in 2 cases.Conclusion Excellent images and synchronism of DSA video are achieved in this system.which can meet clinical requirements of diagnosis and synchronism. Key words: Angiography,digital subtraction; Telemedicine; Evaluation studies

S-Video

Digital subtraction angiography

10.3321/j.issn:1005-1201.2008.03.017

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