O. Bulbena

Institut d'Investigacions Biomèdiques de Barcelona

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Joan Roselló‐Catafau

Institut d'Investigacions Biomèdiques de Barcelona

Ellen Gelpí

Medical University of Vienna

Isabel Ramis

Almirall (Spain)

Daniel Closa

Institut d'Investigacions Biomèdiques de Barcelona

Georgina Hotter

Consejo Superior de Investigaciones Científicas

E. Gelpı́

Institut d'Investigacions Biomèdiques de Barcelona

Ginés Escolar

Hospital Clínic de Barcelona

G. Bioque

Institut d'Investigacions Biomèdiques de Barcelona

Carmen Navarro

San Diego State University

Laureano Fernández‐Cruz

Universitat de Barcelona

Cooperative Institutions

Universitat de Barcelona

Consorci Institut D'Investigacions Biomediques August Pi I Sunyer

Hospital Clínic de Barcelona

Instituto de Salud Carlos III

Institut d'Investigacions Biomèdiques de Barcelona

Centro de Investigación Biomédica en Red

Consejo Superior de Investigaciones Científicas

Universitat Autònoma de Barcelona

Universitat de València

Centro de Investigación Biomédica en Red de Enfermedades Respiratorias

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Effect of Zinc Acexamate and Ranitidine on Chronic Gastric Lesions in the Rat

Digestion (1990)

Carmen Navarro Ginés Escolar M. L. Bravo Elizabeth Yakes Jimenez O. Bulbena

Using the rat as an experimental model we have studied the healing of chronic gastric lesions and the modifications of these lesions by antiulcer agents. Gastric injuries were induced by submucosal injection of 0.05 ml of 5% acetic acid. Placebo, ranitidine (RNT) or zinc acexamate (ZAC) were administered orally. The evolution of gastric injuries was macro- and microscopically evaluated at 6, 12 and 21 days after acetic acid injection. The administration of either RNT (30 mg/kg) or ZAC (200 mg/kg) was followed by a marked improvement of the healing process with respect to control groups. The size of experimental ulcers at 21 days was 3.1 ± 0.8 mm2 for the control group, 1.8 ± 1.1 mm2 for RNT-treated animals and 0.3 ± 0.6 mm2 for ZAC-treated rats (p < 0.05, vs. control). A similar tendency was observed when lesions were microscopically analyzed. Indices of microscopical lesions (0–6) at 21 days were 3.8 ± 0.8 for the control group, 3.0 ± 0.8 for rats receiving RNT and 2.3 ± 0.4 for rats receiving ZAC ( p < 0.05, vs. control). The statistical analysis of the distribution of microscopical indices of lesions showed significant differences in favour of ZAC at days 6 (p < 0.01) and 21 (p < 0.05). Our study indicates that the evolution of gastric damage induced by acid acetic injection was consistently better in rats treated with ZAC than in those receiving RNT. Data obtained in our experiments suggest that the blockade of H2 receptors does not guarantee the optimal healing of chronic gastric lesions induced in rats.

10.1159/000200233

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Citations (18)

Application of totally automated on-line sample clean up system for extraction and high-performance liquid chromatography separation of peptide leukotrienes

Journal of Pharmaceutical and Biomedical Analysis (1993)

Isabel Ramis Georgina Hotter Joan Roselló‐Catafau O. Bulbena César Picado

Solid phase extraction

Sample Preparation

Cartridge

10.1016/0731-7085(93)80094-h

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Citations (7)

Macrophage activation in exacerbated COPD with and without community-acquired pneumonia

European Respiratory Journal (2009)

P. Gutierrez Daniel Closa R. Piñer O. Bulbena Rosario Menéndez

In large series of nonresponding community-acquired pneumonia (CAP) patients, chronic obstructive pulmonary disease (COPD) was observed to be a protective factor for nonresponse to initial antibiotics. This intriguing fact may be linked to changes in the phenotype of inflammatory cells and, in particular, to the induction of classical-M1 or alternative-M2 activation of macrophages, which result in different inflammatory profiles. We evaluated the effect of sputum obtained from patients with acute exacerbation of COPD (AECOPD), CAP and COPD+CAP on the phenotypic changes in macrophages. Human THP1 cells differentiated to macrophages were incubated with sputum from patients with AECOPD, CAP or COPD+CAP, and expression of tumour necrosis factor-α, interleukin-6, mannose receptor and arginase was measured to evaluate the phenotype acquired by macrophages. We found that sputum from CAP patients induced the M1 phenotype and that from AECOPD patients induced an M2-like phenotype. Sputum from CAP+COPD patients did not present a clear M1 or M2 phenotype. These results indicate that the microenvironment in the lung modulates the activation of macrophages, resulting in different phenotypes in AECOPD, CAP and COPD+CAP patients. This different type of activation induces different inflammatory responses and may be involved in the different outcome observed when COPD and CAP present simultaneously.

10.1183/09031936.00118909

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Citations (46)

Constitutive nuclear factor-kappaB activity in human upper airway tissues and nasal epithelial cells

European Respiratory Journal (2000)

Isabel Ramis G. Bioque José A. Lorente Pedro Jares Piera Quesada

Respiratory epithelial cells are actively involved in the host defence and inflammatory reactions of the airways. Nuclear factor-kappaB (NF-kappaB) is a transcription factor that plays a pivotal role in many cellular responses to environmental changes. The inducible nitric oxide synthase (iNOS) isoform has been implicated in airway inflammation as well as in normal airway function. In this study, the hypothesis that NF-kappaB may be associated with iNOS expression in airway epithelium, not only in inflammatory processes but also under physiological conditions was examined. NF-kappaB deoxyribonucleic acid-binding activity was assayed by means of electrophoretic mobility shift assay (EMSA) and iNOS expression examined using immunohistochemical techniques in healthy nasal mucosa and chronically inflamed nasal polyps. Further NF-kappaB activity was assayed; by means of EMSA, in nasal epithelial cells isolated from both tissues. NF-kappaB was activated in nasal polyps, but also to the same extent in healthy nasal mucosa. Uniform iNOS expression was localized within the airway epithelium in both inflamed and noninflamed tissues. Along with iNOS expression, concomitant NF-kappaB activation was found in nasal epithelial cells obtained from both tissues and no differences were observed when nasal mucosa and nasal polyp were compared. These results suggest that constitutive nuclear factor-kappaB and concurrent inducible nitric oxide synthase expression in epithelial cells may play a physiological role in airway function.

Nasal Polyps

Respiratory Mucosa

10.1034/j.1399-3003.2000.15.26.x

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Citations (20)

Relationship between gastric levels and antiulcerogenic activity of zinc.

PubMed (1990)

C Navarro Antonio Ramis Sandra Aixa Kremer Sendros O. Bulbena Lluís Ferrer

The relationship between the absorption of an organic zinc salt, zinc acexamate, and its antiulcerogenic activity in a model of cold-restraint stress was studied. Serum and gastric levels of zinc, as well as its antiulcerogenic effect, were determined after oral or intravenous administration of zinc acexamate. Cytochemical and X-ray microanalysis techniques were also applied. In the rats subjected to cold-restraint stress, gastric levels of zinc correlated with the antiulcerogenic effect observed after administration of zinc acexamate. However, it was not possible to establish a relationship between serum levels and the pharmacologic effect of zinc. Our results in animals subjected to regular diet indicate that the antiulcerogenic effect exhibited by zinc compounds could be associated with the presence of zinc at different levels of gastric tissue.

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Citations (6)

Prostanoid generation in early stages of acute pancreatitis: A role for nitric oxide

Inflammation (1994)

Daniel Closa Georgina Hotter Neus Prats O. Bulbena Joan Roselló‐Catafau

Eicosanoid metabolism

Prostanoid

10.1007/bf01560694

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Citations (21)

Thermospray liquid chromatography/mass spectrometry of prostaglandin methyl ester derivatives: Application to the determination of prostaglandins E2 and D2 in rat gastric mucosa

Biological Mass Spectrometry (1988)

Joaquín Abián O. Bulbena E. Gelpı́

Methyl ester derivatives of 6-keto prostaglandin (PG) F1 alpha, PGF 2 alpha, PGE2 and PGD2 as well as methyl oximes of 6-keto PGF1 alpha, PGE2 and PGD2 have been analysed by thermospray high-performance liquid chromatography mass spectrometry. These compounds are eluted in a gradient of ammonium acetate buffer-acetonitrile at pH 3.4 using a 5 micron ODS-2 reversed-phase column. The mass spectral patterns of the PG derivatives are discussed relative to those of non-derivatized PGs. In general, the methyl ester derivatives show better characteristics than the non-derivatized PGs for the analysis of these compounds in a biological matrix, both from a chromatographic as well as from a mass spectrometric standpoint. Detection limits between 100 and 600 pg on-column can be achieved. Response curves in the selected ion monitoring mode for PGF2 alpha, PGE2 and PGD2 are linear in the range from 100 pg to 10 ng. The technique has been applied to the analysis of PGE2 and PGD2 in rat gastric mucosa.

Thermospray

Dinoprostone

Prostaglandin D2

10.1002/bms.1200160139

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Citations (22)

Gastrotoxic activity and inhibitory effects on gastric mucosal PGE2 production with different non-steroidal anti-inflammatory drugs: Modifications induced by pretreatment with zinc acexamate

Prostaglandins Leukotrienes and Essential Fatty Acids (1994)

Carmen Navarro M. L. Bravo C. Carulla O. Bulbena

Diclofenac

Ketoprofen

Nabumetone