Lianju Ma

Shenyang Normal University

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Co-Authors

Yueying Li

Shenyang Normal University

Lanlan Wang

Shenyang Normal University

Xuemei Li

Chinese Academy of Fishery Sciences

Xuemei Li

Army Medical University

Ning Bu

Second Affiliated Hospital of Xi'an Jiaotong University

Xuhao Wang

Chang'an University

Jinghui Cui

Shenyang Normal University

Lihong Zhang

Liaoning University

Jiahui Fan

Qilu Hospital of Shandong University

Xiufen Qi

Shenyang Normal University

Cooperative Institutions

Shenyang Normal University

Chongqing Medical University

Chinese Academy of Sciences

Chinese Academy of Medical Sciences & Peking Union Medical College

University of Chinese Academy of Sciences

Sichuan University

Ministry of Agriculture and Rural Affairs

Shanghai Jiao Tong University

Zhejiang University

Chinese Academy of Agricultural Sciences

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[Study on the sorption and isolation of shikimic acid by 717 anion exchange resin].

Zhongyaocai (2008)

Lianju Ma Xin Liu Zhuo Yu-juan Lu-Rong Yu

To establish a convenient, practical and environmental method for extracting and isolating shikimic acid.The content of shikimic acid was measured by RP-ion-pair HPLC, the effects of different pH, temperature, sample concentration and eluate concentration on 717 anion exchange resin were studied.The yield of shikimic acid was 96.52% on the condition of 22 degrees C, pH > 6.5, 7.5 mg /ml sample concentration and 0.03 mol/L HCl eluting.This method is feasible and suitable for the extraction and isolation of shikimic acid.

Shikimic acid

Ion-exchange resin

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Citations (1)

717陰イオン交換樹脂によるシキミ酸の吸着分離に関する研究【JST・京大機械翻訳】

Zhongyaocai (2008)

Lianju Ma Liu Xin Zhuo Yujuan Yu Lurong

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Analysis on age structure ofZoysia japonica (Poaceae) population

Journal of Forestry Research (2003)

Yan Wang Dai Bao-qing Liang Yong-jun Lianju Ma

Tiller (botany)

Japonica rice

Age structure

10.1007/bf02856765

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Citations (1)

Isolation and identification of dexamethasone sodium phosphate degrading Pseudomonas alcaligenes

Journal of Basic Microbiology (2014)

Yi Wang Yang ZhiBang Li Zhu Zhongquan Shi Lianju Ma

Glucocorticosteroids such as dexamethasone have polluted hospital wastewater, urban sewage, and river water in varying degrees. However, dexamethasone degradation by bioremediation technology is less understood. This study aims to isolate bacteria that could degrade dexamethasone and to identify their degradation characteristics. Hospital wastewater contaminated by dexamethasone was collected. After culturing in inorganic salt medium and in carbon deficient medium containing dexamethasone sodium phosphate, a bacterial strain with dexamethasone sodium phosphate as the sole carbon and energy source was enriched and isolated from the contaminated wastewater. The strain was identified as Pseudomonas alcaligenes by morphology, Gram staining, biochemical test, and 16S rDNA sequencing. Isolated bacteria were domesticated. Then its degradation characteristic was determined by high‐performance liquid chromatography method. The degradation rate of P. alcaligenes on dexamethasone sodium phosphate was 50.86%. Of the degraded dexamethasone sodium phosphate, 75.23% of dexamethasone sodium phosphate was degraded to dexamethasone and 23.63% was degraded to other metabolites. In conclusion, the isolated P. alcaligenes in this study would provide experimental evidence for further research on the bioremediation technology to treat dexamethasone sodium phosphate and dexamethasone polluted water and further for the elimination of side effects of dexamethasone.

Dexamethasone Sodium Phosphate

Alcaligenes

10.1002/jobm.201300912

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Long Non-Coding Rnas Regulated Glutathione Metabolism in Root Tissue of Endophyte-Infected Rice Seedling Response to Pb Stress

He-Fei Sun Xin-Nan Wang Yanan Li Lanlan Wang Yueying Li

Endophyte

10.2139/ssrn.4900018

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Endophytic Fungus Glomerella sp. JP4 Alleviated the Phytotoxic Effects in Rice Seedlings Exposed to Aluminum Stress

Polish Journal of Environmental Studies (2024)

Xingyue Lin Lili Gao Guiyao Wang Guo Chang Ze Wang

Aluminum (Al) is known to inhibit plant growth and limit crop yields in acid areas.This study aimed to investigate the effect of endophytic fungus Glomerella sp.JP4 infection on the growth, photosynthetic pigments, antioxidant enzyme activities, organic acids contents, and Al content of endophyte-infected rice seedlings (EI) and endophyte-uninfected rice seedlings (EF) exposed to Al stress for 9 days.Al stress decreased shoot height, root length, and dry weight of EF plants.Endophyte infection increased the growth parameters except for root length.Compared to EF plants, the chlorophyll a+b content and carotenoid content were significantly enhanced in the EI plants.Antioxidant enzyme activity was also increased in the EI plants compared to the EF plants subjected to Al stress, while malondialdehyde (MDA) content was remarkably reduced.Endophytic infection significantly increased the contents of citrate and succinate in leaves, as well as that of lactate, malate, fumarate, and succinate in roots under Al stress.Endophyte infection decreased the Al content in the shoots and the roots and restricted the Al transfer from the roots to the shoots in the EI plants compared to the EF plants.Our results indicated that infection with endophytic fungus JP4 in the roots had an active role in promoting plant growth, alleviating the phytotoxic effects caused by Al exposure.

Endophyte

Dry weight

Malondialdehyde

10.15244/pjoes/178319

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Genetic Toxicity of Alginate-Derived Oligosaccharide on Vicia Faba Root Tip Cells

Journal of Shenyang Normal University (2008)

Lianju Ma

The effects of different concentrations of alginate-derived oligosaccharide(ADO) on Vicia faba root tip cells were studied.Micronucleus assay and chromosomal aberration assay were used to determine the mitotic index,the micronucleus rate and chromosomal aberration rate of Vivia faba root tip cells induced by arsenic trioxide under different concentrations of ADO.The result showed that ADO could effectively inhibit the micronucleus rate.During concentration of 10～0.625 g/L,the number of micronucleus of Vivia faba root tip cells were reduced with decrease concentration of ADO,but it was increased when concentration of ADO was lower than 0.625 g/L.The mitotic index was increased,while chromosomal aberration rate were lowered under all concentration of ADO.Turkey test showed all chromosomal aberration rate were significantly different from the control(p0.05).So,ADO has an obvious effect on restraining chromosomal aberration and increasing division of cell of Vivia faba root tip.

Root tip

Mitotic index

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Degradation of dexamethasone by acclimated strain of Pseudomonas Alcaligenes.

PubMed (2015)

Li Zhu Yang ZhiBang Qian Yang Zeng Tu Lianju Ma

This study is to investigate the use of microbial remediation technology for degradation of dexamethasone in polluted water. A strain of Pseudomonas Alcaligenes with the ability of dexamethasone degradation was isolated from hospital polluted water. This strain was further acclimated into a bacterial strain that could highly degrade dexamethasone. Domesticated bacterial proteins were separated by osmotic shock method and were analyzed using SDS-PAGE. Enzyme activity of dexamethasone degradation was detected by high performance liquid chromatography. Protein bands with different molecular weight were found in all regions of the bacteria and a band with molecular weight of about 100 kDa was most obvious. In intracellular and periplasmic liquid, there was a band with molecular weight of about 41 kDa. Enzyme activity mainly existed in intracellular liquid. The 41 kDa protease was purified using ammonium sulfate precipitation, DEAE-52 ion exchange column and Sephadex G-100 column. Dexamethasone and dexamethasone sodium phosphate degrading rates of the purified enzyme were 36% and 95%, respectively. The 100 kDa protein had a 19% coverage rate to TonB receptor dependent protein, with 11 peptides matching. The 41 kDa protein had a 56% coverage rate to isovaleryl coenzyme A dehydrogenase, with 5 peptides matching. The 41 kDa protein had good degradation between the temperature of 25-40°C and PH value of 6.5-8.5. The enzyme kinetics equation was Ct = C0 e(-0.1769t), in accordance with the first-order kinetic equation. This study laid the foundation for further preparation of bioremediation agents for clearance of dexamethasone pollution in water.

Sephadex

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Growth, photosynthesis and antioxidant responses of endophyte infected and non-infected rice under lead stress conditions

Journal of Hazardous Materials (2012)

Xuemei Li Ning Bu Yueying Li Lianju Ma Shigang Xin

Endophyte

Malondialdehyde

Dry weight

10.1016/j.jhazmat.2012.01.052

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