Objective To investigate the relationships of testosterone,luteinizing hormone and estrodiol with NT-proBNP in old women and men.Methods 226 healthy individuals(134 women and 92 men),aged 60 to 70 years,were selected from Nanfang Hospital.The levels of testosterone,luteinizing hormone,estrodiol and NT-proBNP were examined.Basic clinical data were evaluated,including age,serum creatinine and body mass index(BMI).According to the level of NT-proBNP,the women and men were divided into two groups(the lower group:NT-proBNP125 pg/ml and the higher group:125 pg/ml≤NT-proBNP400 pg/ml).Results There was no significant difference in luteinizing hormone and estrodiol between the lower group and the higher group either in women or men(P0.05).Compared with that of the lower group,the level of testosterone was decreased in the higher group(P=0.000 in men,P=0.003 in women and P=0.003 in the whole individuals).There was no correlation between luteinizing hormone and estrodiol and NT-proBNP in men,women and the whole individuals(P0.05).There was negative correlation between testosterone and NT-proBNP(r=-0.318,P=0.002 in men;r=-0.312,P=0.000 in women;and r=-0.217,P=0.001 in the whole individuals).Conclusions There is negative correlation between testosterone and NT-proBNP in healthy old people.Maybe testosterone is one of the key factors which cause the NT-proBNP level in healthy elderly women significantly to be higher than healthy men of the same age.
Recent studies have shown that Bombyx adult brain contains two types of PTTHs with different molecular weights, 4K-PTTH and 22K-PTTH. The 4K-PTTH is composed of three heterogeneous molecular species, 4K-PTTH-I, -II and -III and their NH2-terminal amino acid sequences have been determined (Nagasawa et al., 1984). It is of great interest that the sequences of the 4K-PTTHs contain regions homologous with that of insulin A chain, showing that Bombyx 4K-PTTH and vertebrate insulin are homologous in structure. What are the functions of 4K-PTTH and vertebrate insulin ? To determine if vertebrate insulin have a 4K-PTTH activity, we used Indian silkworm, Philosamia cynthia ricini, debrained pupae as experimental animal to examine its activity.
AIM: To investigate the changes of glutamate (Glu) in brain of rats exposed to infrasound. METHODS: Rat model was established by putting the rats in the environment of infrasound for two hours. The rats were divided into five groups: normal control, 8 Hz/90 dB, 8 Hz/120 dB, 16 Hz/90 dB and 16 Hz/120 dB. RESULTS: Compared with the control group, the content of Glu in cerebral hippocampus was higher in 8 Hz/120 dB group and 16 Hz/120 group(P0.01);The elevation of Glu content in 16 Hz/120 dB group was more significant than that in 8 Hz/120 dB group (P0.01). HZCONCLUSION: The infrasound effect on the brain is related to the frequency and sound grade, and the latter is the key factor. The exciting toxicity of Glu may play a more important role in the injury of brain by infrasound.
The encoding region of mpd gene for methyl parathion hydrolase was subcloned by PCR. The recombinant plasmid pET29a-mpd was constructed and the methyl parathion hydrolase was expressed in E. coli BL21 (DE3) as a fusion protein tagged with (His) 6 at C-terminus. The fusion protein was purified to homogeneity by Ni-affinity chromatography under undenaturing condition, the protein could degrade methyl parathion effectively. A convenient and reliable method to assay the activity of methyl parathion hydrolase was established. The effects of environmental factors on the activity of methyl parathion hydrolase and its enzymatic kinetics were analyzed. The optimum pH value for enzymatic hydrolysis of methyl parathion was 8 6～8 8, the optimum temperature was 15℃. Mn 2+, Zn 2+, Cu 2+ increased the activity of methyl parathion hydrolase by 15%～20%. Ca 2+, Mg 2+ and Ni 2+ decreased enzyme activity slightly. 1mmol/L EDTA·Na 2+ almost had no effect on the enzyme activity, but 10mmol/L EDTA·Na 2+ inhibited enzyme activity strongly. The K m and kcat against methyl parathion at 25℃ was (68 6 ± 5 1)μmol/L and (45 ± 6)S -1 respectively. The optimum pH value for hydrolysis of parathion by this hydrolase was also 8 6～8 8, the K m and kcat against parathion at 25℃ was (59 5 ± 6 0)μmol/L and (8 ± 1) S -1 respectively. The values of kcat/K m of methyl parathion hydrolase to two substrates showed that the enzyme hydrolyzes methyl parathion more efficiently.
Objective:To study the expression of intercellular adhesion molecule 1 in rat heart with ischemia /reperfusion injury and its association with ischemia/reperfusion injury.Methods:Fourty two male Sprague Dawley rats were subjected to coronary artery ligation as ischemia/reperfusion heart model and were randomly divided into seven groups of six rats each.The ischemia time was 30 min.The rat hearts were excised at 0,30,60,360,540,720 min after reperfusion.The ischemic and reperfused myocardium was examined immunohistochemically to localize ICAM 1.Malondialdehyde (MDA) and nitric oxide (NO) contents in the myocardium were also determined by thiobarbituric acid and Griss method,respectively.Results:①At the time of 540 min after reperfusion,expression of ICAM 1 in rat hearts was upragulated.②The MDA values were increased during reperfusion ( P 0.05 ) and reached the peak 1 hour after reperfusion.③The NO values get lower and lower during reperfusion.Conclusion:Expession of ICAM 1 in rat hearts subjected to ischemia followed by reperfusion was increased in the late stage of reperfusion,which might be associated with delayed reperfusion injury.
AIM: In order to study the properties of C1 peptide(band 3 Ala893-Va1911).METHODS:The human erythrocyte white ghost were pre-treated by 100 mmol/L NaOH and then digested by trypsin. Using the HPLC and amino acid sequencer, we identified the peptides that released from white ghost, and purified the C1 peptides(band 3 Ala893-Va1911). The C1 peptides were used to incubate with fresh white ghosts.RESULTS:C1 peptides related with a novel protease activity.
Organophosphate chemicals are widely used as agricultural pesticides and war reagents, their biodegradation is emphasized on the theoretical and practical aspects. Organophosphate hydrolases play important roles in the biodegradation of organophosphate chemicals. Great advancement was achieved recently in the determination of crystal structure and catalytic mechanisms of the hydrolase. This paper reviewed the research progresses in the enzymology, protein structure, catalytic mechanisms and application of the organophosphate hydrolase, and predicted the future research in this field.
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