Tan Jian-hua

Guangdong Testing Institute for Product Quality Supervision

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Wang JiCai

Guo ChangHong

Guangdong Testing Institute for Product Quality Supervision

Peihua Liu

China National Petroleum Corporation (China)

Chen QiaoHua

Guangzhou Quality Supervision, Inspection and Research Institute

Xi ShaoFeng

Guangzhou Quality Supervision, Inspection and Research Institute

Zebo Huang

Guangdong Pharmaceutical University

Jingjing Hu

East China Normal University

Yongdong Zou

Shenzhen University

Qiangqiang Wang

South China University of Technology

Shen CongWen

Cooperative Institutions

Guangzhou Quality Supervision, Inspection and Research Institute

Chinese Academy of Sciences

South China University of Technology

Xiamen University

Jilin University

Chinese Academy of Medical Sciences & Peking Union Medical College

Sun Yat-sen University

Beihang University

Shanghai Jiao Tong University

China Agricultural University

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High-level secretion and purification of recombinant acetylcholinesterase from human cerebral tissue in P. pastoris and identification by chromogenic reaction

Applied Microbiology and Biotechnology (2006)

Xingyuan Ma Tan Jian-hua Dongzhi Wei Zhu Pin Manji Sun

Chromogenic

Pichia

10.1007/s00253-005-0260-y

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Physicochemical characterization and cosmetic application of kelp blanching water polysaccharides

International Journal of Biological Macromolecules (2023)

Peihua Liu Jingjing Hu Qiangqiang Wang Tan Jian-hua Jian Wei

Blanching

Biopolymer

Alginic acid

Exopolymer

10.1016/j.ijbiomac.2023.125981

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Determination of polychlorobiphenyls in paper-made food packaging containers by gas chromatograph-mass spectrometry.

Chen QiaoHua Guo ChangHong Tan Jian-hua Wang JiCai Shen CongWen

Determination of polychlorinated biphenyls (PCBs) by GC-MS method showed good sensitivity and selectivity. In determining paper-made food packaging containers and materials,samples can be analyzed directly only after solvent extraction and concentration without any cleanup procedure. The detection limits ,average recoveries and RSD of the method were 1μg/kg for target compounds,≥95% and 4.25-10.34%,respectively.

Accelerated solvent extraction

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Effect of GnRH Impulse on the Expression of FSHβ mRNA and LHβ mRNA in Rats' GTH Cells Primarily Cultured in Vitro

Tan Jian-hua

The article aims to explain at molecular level the effect of gonadotropin releasing hormone(GnRH) at different impulse frequencies on the secretion of follicle stimulating hormone(FSH) by gonadotropin(GTH).The change of FSHβ mRNA in cells was researched when the pituicytes of original generation of rats cultured in vitro were stimulated by GnRH at different impulse frequencies,by applying the technique of culture in vitro of cells in combination with the fluorescent quantitation of PCR.The result shows that in the condition of the same vibration amplitude,when the cells were stimulated by impulse at low frequency(at the interval of 120 min),the level of FSHβ mRNA reached the top.This supports the hypothesis that GnRH impulse frequency itself is a regulation signal.

Gonadotropin

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Respective effects of LPS and LT on the intestinal mucous membrane microvascular in vitro

Xin Wang Tan Jian-hua DU Shao-fan

The intestinal mucosa microvascular endothelial cells were divided into blank control group,BSA control group,LPS1 group,LPS2 group,LT1 group and LT2 group.By adding feeding medium 50 μg/mL BSA,1 μg/mL LPS,10 μg/mL LPS,10 μg/mL LT and 50 μg/mL LT culture fluid respectively into the 6 groups to cultivate statically for 12 hours,the respective effects of different concentrations of LPS and LT on the intestinal mucous membrane microvascular endothelial cells' shape and excreting NO was studied,then the model dosage of LPS and LT damaging intestinal mucosa microvascular endothelial cells in rats was decided.LPS makes the ratio between the length and the width of a cell increase and the gaps between cells wider;LT makes the cells swell and the alignment in disorder.Both LPS and LT of every concentration can lead to the rising of the secretion of NO by intestinal mucosa microvascular endothelial cells,but the function of 1 μg/mL LPS and 50 μg/mL LT is more obvious.The model dosage of LPS and LT damaging intestinal mucosa microvascular endothelial cells in rats is respectively 1 μg/mL and 50 μg/mL.

Intestinal mucosa

Intestinal villus

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Determination of formaldehyde residues in paper-made food containers.

Huiyong Li Yanming Zheng Tan Jian-hua Wang JiCai Chen QiaoHua

Formaldehyde in paper-made food containers was extracted by different solvents,including cold water,hot water and 4% acetic acid.These three liquid show different abilities to extract formaldehyde from paper-made food packaging materials:4% acetic acidhot water cold water.About 60 kinds of paper-made food containers were sampled to determinate formaldehyde.It was found that these containers without the inside layer coating had higher contents of formaldehyde,with the average concentration being of 0.93 mg/L.And those with inside layer coating had comparatively low contents of formaldehyde,about 0.20mg/L.

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Clinical observation of clarithromycin treatment for nasosinusitis after nasopharyngeal carcinoma radiotherapy

Journal of clinical otorhinolaryngology, head, and neck surgery (2010)

Zunbin Ke Tang Rongde Tan Jian-hua Zhijian Jiang Zhifang Liang

Objective:To observe the clinical treatment effectiveness of clarithromycin for nasosinusitis after nasopharyngeal carcinoma radiotherapy.Method:Twenty-four cases of nasosinusitis after one year nasopharyngeal carcinoma radiotherapy were treated with the oral clarithromycin of small dosage(250 mg everyday).The course of treatment were for 12 to 20 weeks.The treatment effectiveness were evaluated with the chronic nasosinusitis visual analog quality table mark system(VAS)and Lund-Mackay nasal sinuses CT image mark system before and after treatment.Result:The VAS mark and CT image mark have significant deviation(P0.01) among pretherapy,post-treament and 6 months after drug withdrawal.No significant deviation was found(P0.05) between post-treament and 6 months after drug withdrawal. The treatment effectiveness was judged for very good is 9 cases, for the good is 11 cases and for the bad is 4 cases.Conclusion:The clarithromycin of small dosage for long-term treatment of nasosinusitis after nasopharyngeal carcinoma radiotherapy has positive clinical treatment effectiveness,which is a better treatment method.

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Effects of Orchidectomy on Gonadotrophs in the Anterior Pituitary and Plasma Gonadotropin Levels of Stallions

Chinese Journal of Veterinary (2004)

Tan Jian-hua

The number and percentage and size of gonadotrophs in anterior pituitary from 7 adult stallions and 6 adult geldings orchidectomized at 1-2 years were observed with immunohistochemistry and morphometry by light microscopy.The plasma concentrations of FSH and LH were also measured by radioimmunoassay(RIA).The results showed that prepubertal orchidectomy of the equine resulted in a slight but not significant increase in the number and percentage of gonadotrophs,but significantly increased the average area of gonadotrophs,and the levels of plasma gonadotropins also rised.These results indicated that,during postnatal life,the equine testes may suppress the synthesis, storage and release of gonadotropins in the anterior pituitary.

Gonadotropin

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液体クロマトグラフィー‐質量分析によるヒト尿中の多環芳香族炭化水素のヒドロキシル化関連代謝産物の準標的解析【Powered by NICT】

Journal of Chromatography A (2016)

Tang Caiming Tan Jian-hua Ruifang Fan Bo Zhao Tang Caixing

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Identification of Four Active Constituents in Hair Growth Cosmetics by Ultra Performance Liquid Chromatography Coupled with Quadrupole Time - of - Flight Mass Spectrometry

Fenxi Ceshi Xuebao (2016)

Xie Wenjian Xiaoting Xiong Xi Shaofeng Huiyong Li Tan Jian-hua

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