Objective To evaluate the value of microplate hybridization assay and PCR enhanced chemiluminescence (ECL) in the detection of Mycobacterium tuberculosis . Methods 60 sputum specimens from patients with active pulmonary tuberculosis and 54 sputum specimens from people without tuberculosis were tested by PCR ECL, PCR colorimetry and PCR agarose gel electrophoresis respectively. The results were compared with each other. Results The positive rates of PCR ECL, PCR colorimetry and PCR agarose gel electrophoresis were 76.7%,63.3% and 50.0% respectively. There was a significant difference between the positive rates of PCR ECL and PCR agarose gel electrophoresis( P 0.05 ). In the smear positive specimens, there was no significant difference among the positive rates of three methods( P 0.05). In the smear negative specimens, the positive rates of three methods were 73.9%,56.5% and 43.5% respectively. There was a significant difference between the positive rates of PCR ECL and PCR agarose gel electrophoresis( P 0.05). The specificity of PCR ECL, PCR colorimetry and PCR agarose gel electrophoresis were 92.6%, 96.3 % and 96.3% respectively. There was no significant difference among them( P 0.05 ). Conclusion PCR ECL was sensitive and useful, especially in the detection of smear negative specimens. Furthermore, the method was easily performed and the interpretation of results was objective.
Background: The incidence and mortality of lung cancer are the highest in the world, and the most lesions are located in lung periphery. Despite the greatest degree of screening and diagnosis, the pathologic types of peripheral pulmonary lesions (PPLs) are difficult to diagnose by noninvasive examination. Here, we found a novel index time difference of arrival (TDOA) to discriminate between benign and malignant lesions of PPLs. Methods: We retrospectively analyzed 96 patients with PPLs using the contrast-enhanced ultrasound (CEUS), who had undergone biopsy to confirm the pathologic types. All the data collected from Dazhou Central Hospital between December 2012 and July 2019. The parameters of CEUS analyzed by two assistant chief physicians of ultrasound diagnosis. Area under the receiver operating characteristic curve analysis, sensitivity, specificity, positive predictive value and negative predictive value were performed to assess the diagnostic ability of different indices. Findings: We found that the TDOA significantly distinguished benign from malignant lesions. The TDOA was markedly increased in patients with malignant lesions than benign lesions (P<0*001). Compared with conventional time-intensity curve (TIC) indices, the TDOA showed high diagnostic accuracy (area under the curve, 0*894). Moreover, conventional diagnostic indices did not affect the diagnostic performance of TDOA by adjusted the receiver operating characteristic curve. Interpretation: Our results suggest that the TDOA is feasible for the diagnosis of the patients with PPLs. Funding Statement: This article was funded by the National Natural Science Foundation of China (81902861), the Scientific Research Fund of Sichuan health and Health Committee (No. 18PJ361) and the Scientific Research Fund of Technology Bureau in Sichuan Province (No. 2018138, No. 2018JY0324). Declaration of Interests: All authors declare no competing interests of this study.Ethics Approval Statement: The study was approved by the Ethics Committee of Dazhou Central Hospital, Sichuan, China.
Objective: To investigate the clinical value of combined detection of serum miR-378 and miR-21 in gastric cancer (GC). Methods: Eighty-seven patients with GC and 78 patients with colorectal cancer(CRC) from National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences were selected, 83 individuals undergoing healthy physical examination were selected as the healthy controls. The levels of serum miR-378 and miR-21 were detected by quantitative real-time PCR (RT-qPCR) (result data were transformed as log2 for analysis). Results: Relative expression levels of miR-378 in the serum were -1.24, -3.25 and -2.73 in healthy controls, GC and CRC patients, respectively. Compared with the healthy controls, the levels of serum miR-378 were significantly decreased in GC and CRC patients (both P<0.05). Relative expression levels of miR-21 in the serum were 0.11, 2.34 and 2.47 in healthy controls, GC and CRC patients, respectively. Compared with the healthy controls, the levels of serum miR-21 were significantly up-regulated in GC and CRC patients (both P<0.05). Moreover, the serum level of miR-378 in GC patients was inversely associated with tumor clinical stage (P<0.05). However, the level of miR-21 showed no significant differences among patients with different clinical and pathological characteristics (all P>0.05). The area under the receiver operating characteristic curve (AUC), sensitivity and specificity of miRNA-378 to diagnose GC was 0.770, 82.0% and 66.0%, respectively, and were 0.900, 85.0%, and 88.0% of miR-21, respectively. The AUC, sensitivity and specificity of combined detection of serum miR-378 and miR-21 to diagnose GC were 0.930, 92.0% and 87.0%, respectively, while the AUC of combined detection of serum CEA and CA-199 was 0.767, the AUC of combined all of the four factors was 0.946. Conclusion: The combined detection of serum miR-378 and miR-21 have a certain effect on diagnosis of GC.目的： 探讨血清miR-378和miR-21联合检测对胃癌的临床诊断价值。 方法： 选择中国医学科学院肿瘤医院经病理确诊的胃癌患者87例，结直肠癌患者78例，健康体检者83例，采用实时荧光定量PCR法检测血清样本中miR-378和miR-21的表达水平(以log(2)对结果进行转换)。 结果： miR-378在健康对照者、胃癌患者和结直肠癌患者血清中的相对表达水平分别为－1.24、－3.25和－2.73，miR-378在胃癌和结直肠癌患者血清中的相对表达水平明显低于健康对照者(均P<0.05)。miR-21在健康对照者、胃癌患者和结直肠癌患者血清中的相对表达水平分别为0.11、2.34和2.47，miR-21在胃癌和结直肠癌患者血清中的相对表达水平明显高于健康对照者(均P<0.05)。胃癌患者血清中miR-378的低表达与肿瘤的临床分期有关(P<0.05)，分期越晚，下降程度越大；而miR-21在胃癌患者血清中的升高程度与患者的临床病理特征无关(均P>0.05)。血清miR-378诊断胃癌的曲线下面积(AUC)为0.770，灵敏度为82.0%，特异度为66.0%；miR-21诊断胃癌的AUC为0.900，灵敏度为85.0%，特异度为88.0%；miR-378＋miR-21诊断胃癌的AUC为0.930，灵敏度为92.0%，特异度为87.0%。CEA＋CA-199诊断胃癌的AUC为0.767，CEA＋CA-199＋miR-378＋miR-21诊断胃癌的AUC为0.946。 结论： 联合检测血清中miR-378和miR-21的表达对胃癌具有一定的诊断效能。.
Objective To evaluate the prospect of real-time PCR in the clinical diagnosis and efficiency evaluation of the pulmonary tuberculosis treatment.Methods Real-time PCR was established to detect M.tuberculosis specific sequence IS6110 in the sputum samples taken from suspected and diagnosed tuberculosis patients.The result was compared with acid-fast staining and cultivation Result The sensitivity of real-time PCR was 4 copies/reaction,that was significantly higher than acid-fast staining and cultivation.The positive rate of real-time PCR,acid-fast staining and cultivation.was 64.29%,35.12% and 12.5%.The copy of TB diminished obviously in the treatment follow-up.Conclusion The real-time PCR has high sensitivity and specificity in the detection of M.tuberculosis.The method could feedback the efficiency rapidly and in time,and provide evidence for the further treatment from doctors.
Objective:To study the relationship between p34、cyclinB1 proteins and the grade,stage of the bladder carcinoma,and evaluate whether these proteins can serve as markers to describe the biological behavior of bladder carcinoma.Methods:Immunohistochemical methods(S-P) was used to detect the txpression of p34 and cyclin B1.Results:The positive rates of p34 and cyclinB1were 69.4% and 75.5% in transitional cell carcinoma of bladder respectively,and compared with mucosa in paracarcinoma and normal mucous of urinary bladder,there was significant diference(P0.05),and has correlation with tumor,s grade and stages.Conclusion:p34 and cyclinB1 play important role in the development of transitional cell carcinoma of bladder.Detecting p34 and cyclinB1 maybe have benefit to predict the prognosis of transitional cell carcinoma of bladder.
Worldwide, the incidence and mortality of lung cancer are at the highest levels, and the most lesions are located in the lung periphery. Despite extensive screening and diagnosis, the pathologic types of peripheral pulmonary lesions (PPLs) are difficult to diagnose by noninvasive examination. This study aimed to identify a novel index-time difference of arrival (TDOA)-to discriminate between benign inflammation and malignant PPLs.Using contrast-enhanced ultrasound (CEUS), we retrospectively analyzed 96 patients with PPLs who had undergone biopsy to confirm the pathologic types. All data were collected from Dazhou Central Hospital between December 2012 and July 2019. The parameters of CEUS were analyzed by two assistant chief physicians of ultrasound diagnosis. Area under the receiver operating characteristic curve analysis, sensitivity, specificity, positive predictive value, and negative predictive value were calculated to assess the diagnostic ability of different indices.We found that the TDOA significantly distinguished benign inflammation from malignant lesions. The TDOA was markedly increased in patients with malignant lesions than benign inflammation lesions (P < 0.001). Compared with conventional time-intensity curve (TIC) indices, TDOA showed high diagnostic accuracy (area under the curve = 0.894). Moreover, conventional diagnostic indices did not affect the diagnostic performance of TDOA by adjusting the receiver operating characteristic curve.TDOA is feasible for the diagnosis of benign inflammation and malignant PPLs.
Objective To establish a standard empirical process of immunohistochemical staining and slice making of nasopharynx tissues catering to the requirement for pathological diagnosis and research in the future.Methods Introducing the methods of immunohistochemical staining and slice making of nasopharynx tissues as well as the link of quality control in detail. Results The results shown that method has three features: stable and reliable, strong specificity, and high sensitivity. Conclusion This method can improve the quality ofimmunohistochemical staining of nasopharynx tissue.
Objective To study the expression of E-cadherin and β-catenin in nasal inverted papilloma( NIP) and the relationship between the expression and clinicopathologic features of this tumor. Methods Immunohistochemical technique was used for detecting the expression of E-cadherin and β-catenin in 40 specimens of NIP,15 of inferior turbinate( IT) and 15 of nasal squamous cell carcinoma( NSCC). The relationships between the expression levels of Ecadherin,β-catenin and the clinicopathologic characteristics were then analyzed. Results 1The expression of E-cadherin and β-catenin in NIP was significantly higher than that in IT,but lower than in NSCC. The differences were statistically significant( all P 0. 05). Conclusion The expression of E-cadherin and β-catenin in NIP is aberrant,which is closely related with differentiation of the tumor.
To evaluate the therapeutic effects of H(1) blocker in combination with low dose inhaled corticosteroid on allergic asthma.A multi-center, double blind, randomized, placebo control study was conducted in 67 patients with mild to moderate allergic asthma. Patients were randomized to receive either Loratadine 10 mg or placebo twice a day on the basis of inhaled beclomethasone dipropionate (400 microg/d for 14 days, then reduced to 200 microg/d) for 5.3 +/- 1.3 months. Symptom scores of asthma, frequencies of episode of rhinitis and common cold and doses of inhaled Salbutamol as rescue drug were recorded. Bronchial hyperresponsiveness (PD(20) FEV(1) in response to Histamine) and serum ICAM-1 and VCAM-1 were measured before and after the treatment.After treatment, there was much better improvement in symptom score (2.4 +/- 0.9 vs 3.1 +/- 0.9, P < 0.01), symptomatic days due to rhinitis (4.0 +/- 1.2 d/week vs 1.9 +/- 0.9 d/week, P < 0.001), episode of common cold symptom (0.8 +/- 0.5 time vs 1.1 +/- 0.4 time, P < 0.001), average doses of inhaled beta(2) agonist as rescue medication (2.6 +/- 0.9 puff/week vs 3.7 +/- 0.8 puff/week, P < 0.001) and bronchial responsiveness (P < 0.05) in Loratadine group as compared with the control group. However, there was no significant change in serum ICAM-1 and VCAM-1 levels after treatment in both groups (P > 0.05).On the basis of low dose inhaled corticosteroid, orally administered Loratadine significantly improves the therapeutic efficacy of asthma in patients with allergic asthma and rhinitis.
'/%3e%3cuse xlink:href='%23a' transform='rotate(23.036 .093 25.536)'/%3e%3cuse xlink:href='%23a' transform='rotate(45.87 1.273 16.18)'/%3e%3cuse xlink:href='%23a' transform='rotate(69.945 .996 12.078)'/%3e%3cuse xlink:href='%23a' transform='rotate(20.66 -19.689 31.932)'/%3e%3c/svg%3e)