Colin J. Brady

TRIA Bioscience (United States)

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Jim Speirs

Commonwealth Scientific and Industrial Research Organisation

Michael Tasch

TRIA Bioscience (United States)

Tracy Saveria

TRIA Bioscience (United States)

James Roberts

TRIA Bioscience (United States)

Anissa Martinez

Lumen (United Kingdom)

Troy Paddock

National Renewable Energy Laboratory

Caitlin Gamble

Columbia University

Lauren Goetsch

University of Wisconsin–Madison

Mesfin Gewe

Fred Hutch Cancer Center

Jeremy Ferrara

TRIA Bioscience (United States)

Cooperative Institutions

Agricultural Research Organization

Hebrew University of Jerusalem

Commonwealth Scientific and Industrial Research Organisation

Macquarie University

Tel Aviv University

University of Washington

Ministry of Agriculture and Rural Development

TRIA Bioscience (United States)

Weizmann Institute of Science

Ben-Gurion University of the Negev

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Interactions between the Amount and Molecular Forms of Polygalacturonase, Calcium, and Firmness in Tomato Fruit

Journal of the American Society for Horticultural Science (1985)

Colin J. Brady W. B. McGlasson Jessica Pearson S. K. Meldrum E. Kopeliovitch

Abstract A range of tomato ( Lycopersicon esculentum Mill.) cultivars was examined for changes during ripening in firmness, endopolygalacturonase (PG) activity and the molecular forms of polygalacturonase, Ca concentration, and the extractability of the Ca. Firm cultivars were firmer than the soft cultivars throughout ripening, and generally they contained less PG activity at each stage examined. In all cultivars, PG was predominately or entirely in the high molecular weight form (PG1) early in ripening, with the PG2 forms being increasingly prominent as ripening progressed. Differences in firmness were established while PG1 was the predominant PG. Uronic acid polymers in isolated cell walls were degraded rapidly by endogenous PG when citrate was present to complex Ca. In the presence of sufficient citrate, cell wall uronic acids of a firm and soft cultivar were equally susceptible to hydrolysis, suggesting that differences in the digestion of the walls by PG were dependent upon differences in Ca content or distribution. However, neither total, water, nor saline-extractable Ca showed consistent correlations with fruit firmness, and they also showed no progressive change during ripening.

Lycopersicon

Uronic acid

10.21273/jashs.110.2.254

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Exopolygalacturonase protein accumulates late in peach fruit ripening

Physiologia Plantarum (1992)

C.G. Downs Colin J. Brady Andrew A. Gooley

10.1034/j.1399-3054.1992.850203.x

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Purification of three pectin esterases from ripe peach fruit

Phytochemistry (1994)

Hilary E. Glover Colin J. Brady

Pectin

Esterase

Pectinesterase

10.1016/s0031-9422(00)89509-6

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Citations (26)

The response of plant mitochondria to media of high solute content

Journal of Bioenergetics and Biomembranes (1976)

L. C. Campbell John K. Raison Colin J. Brady

Malate dehydrogenase

10.1007/bf00748958

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The Significance of Vascular Connection in Regulating Senescence of the Detached Flag Leaf of Wheat

Journal of Experimental Botany (1983)

Hamid Lazan E.W.R. Barlow Colin J. Brady

The relationship between the accumulation of soluble substances and the senescence of a detached flag leaf of wheat in the light was investigated. With respect to transport to and from the leaf, a detached flag leaf can be considered as either an ‘open’ or a ‘closed’ system. A closed system was obtained by cutting the flag leaf at the base of the leaf sheath, thus severing the vascular connection between the leaf and the stem. An open system was prepared by excising the wheat stem, below the flag leaf insertion node, thereby preserving vascular connections between the flag leaf, stem and ear. By varying the number of nodes left on the stem, or having the ear either intact or detached, soluble carbohydrates and soluble nitrogenous compounds were induced to accumulate within the leaf blade at different rates. Treatments which restricted transport of carbohydrate and nitrogen, out of the detached leaf were found to promote senescence. Senescence was most rapid when the leaf system was ‘closed’ and was considerably delayed when the system was ‘open’ and the ear intact. The onset of senescence was closely associated with the attainment of a threshold concentration of ethanol-soluble carbohydrate in the leaf, while the rate of senescence was modified by the number of attached nodes or by exogenous cytokinin treatments.

Senescence

Flag (linear algebra)

10.1093/jxb/34.6.726

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Citations (35)

Salt-tolerance in plants. I. Ions, compatible organic solutes and the stability of plant ribosomes

Plant Cell & Environment (1984)

Colin J. Brady T. S. Gibson E.W.R. Barlow Jim Speirs R. G. Wyn Jones

10.1111/j.1365-3040.1984.tb01857.x

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Messenger RNA Changes in Tomato Fruit Pericarp in Response to Propylene, Wounding or Ripening

Journal of Plant Physiology (1987)

Elaine E. Lee Jim Speirs W. B. McGlasson Colin J. Brady

Lycopersicon

Polysome

10.1016/s0176-1617(87)80086-x

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Citations (10)

Polyacrylamide gels with modified cross-linkages

Analytical Biochemistry (1976)

P.B.H. O'Connell Colin J. Brady

Polyacrylamide

10.1016/0003-2697(76)90264-5

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Citations (67)

Immunolocalisation of pectinases in tomato fruit

New Zealand Journal of Crop and Horticultural Science (1992)

Barry J. Pogson Graham B. Seymour Colin J. Brady Merfyn Jones D. J. Goodchild

Abstract The distribution of pectin methyl esterase (PE) and endopolygalacturonase (PG) was studied in tomato (Lycopersicon esculentum Mill) fruit. Antibodies were raised in rabbits to the PE2 isoform of PE and the PG2 isoform of PG. The antisera were used to probe PE and PG in sections of mature green and ripening tomato pericarp tissue by immunocytology. PE was detected in the cell wall of green and ripening fruit. A comparison with specific and non-specific sera indicated that the PE antigen was largely confined to the cell wall. PG was detected in the cell wall of ripening but not green tissue. Gold label was observed over both the primary wall and the middle lamella, and also over the cytoplasm. In the wall region, no distinctions could be drawn between the distributions of PE and PG, but PG differed from PE in that a significant proportion of the antigen was in the cytoplasm.

Middle lamella

Lycopersicon

Pectin

Pectinesterase

10.1080/01140671.1992.10421907

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Structure of the tomato Adh2 gene and Adh2 pseudogenes, and a study of Adh2 gene expression in fruit

Plant Molecular Biology (1994)

Terry Longhurst Elizabeth Lee Rick Hinde Colin J. Brady Jim Speirs

Pseudogene

genomic DNA

Stop codon

10.1007/bf00040690

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Citations (68)