A. M. J. J. Verweij-Van Vught

Vrije Universiteit Amsterdam

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D. M. MacLaren

Vrije Universiteit Amsterdam

F. Namavar

Amsterdam UMC Location VUmc

Ben J. Appelmelk

Amsterdam University Medical Centers

L. G. Thijs

Vrije Universiteit Amsterdam

W. A. C. Vel

Vrije Universiteit Amsterdam

Janneke J. Maaskant

Vrije Universiteit Amsterdam

M. Sparrius

Amsterdam UMC Location VUmc

A. M. Simoons‐Smit

Sint Lucas Andreas Hospital

W. F. Schouten

Vrije Universiteit Amsterdam

Ben R. Otto

Vrije Universiteit Amsterdam

Cooperative Institutions

Vrije Universiteit Amsterdam

University of Amsterdam

Amsterdam UMC Location VUmc

Academic Medical Center

OLVG

Erasmus University Rotterdam

University College London

Maastricht University

The Royal Free Hospital

Radboud University Nijmegen

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Effect of Bacteroides fragilis cellular components on chemotactic activity of polymorphonuclear leukocytes towards Escherichia coli

Journal of Medical Microbiology (1987)

F. Namavar A. M. J. J. Verweij-Van Vught Munita Bal D. M. MacLaren

Summary. Chemotaxis of polymorphonuclear leukocytes (PMNL) in response to cell components of Bacteroides fragilis alone or in combination with Escherichia coli was evaluated. E. coli produced much more powerful chemotactic factors than B. fragilis. The culture filtrate (CF), outer membrane (OM) preparation, and lipopolysaccharide (LPS) of B. fragilis slightly stimulated chemotactic activity of PMNL. The culture filtrate and OM preparation were capable of inhibiting the chemotaxis of PMNL in response to the chemotactic factors of E. coli but LPS of B. fragilis was not able to do so. Reduction by B. fragilis of PMNL chemotaxis in response to E. coli was not specific for B. fragilis but also occurred in the presence of facultative bacteria. In parallel with chemotaxis, lysozyme release, but not β-glucuronidase release, by PMNL was significantly stimulated by E. coli but not by B. fragilis.

Bacteroides fragilis

Bacteroidaceae

10.1099/00222615-24-2-119

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Citations (8)

HETEROGENEITY OF ESCHERICHIA COLI J5 VACCINES

The Lancet (1986)

Ben J. Appelmelk N T Rapson A. M. J. J. Verweij-Van Vught Janneke J. Maaskant T. A. M. Hekker

10.1016/s0140-6736(86)92695-4

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The role of K antigens in the phagocytosis of Escherichia coli

Antonie van Leeuwenhoek (1983)

A. M. J. J. Verweij-Van Vught F. Namavar P. G. H. Peerbooms D. M. MacLaren

10.1007/bf00399861

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Restriction Endonuclease DNA Analysis ofBacteroides fragilis

Microbial Ecology in Health and Disease (1991)

F. Namavar T. J. M. van Steenbergen A. M. J. J. Verweij-Van Vught D. M. MacLaren

Hind III and Bam HI restriction endonuclease digests of genomic DNA of 25 Bacteroides fragilis strains, isolated from faeces, intestinal mucosa and infections were investigated. DNA analysis of single isolates from 10 patients with a B. fragilis infection revealed 10 different DNA profiles. DNA isolated from all 15 strains from faeces and intestinal mucosa of one patient showed identical patterns, confirming occurrence of one dominant strain in an individual. Restriction endonuclease DNA analysis provides a sensitive and useful technique for the typing of B. fragilis in epidemiological studies. Keywords: Bacteroides fragilis ; Epidemiology; Restriction endonuclease.

Bacteroides fragilis

Deoxyribonucleases

10.3402/mehd.v4i2.7616

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Citations (2)

Pathogenesis of mixed infections by Escherichia coli and Bacteroides species

Antonie van Leeuwenhoek (1985)

W. A. C. Vel A. M. J. J. Verweij-Van Vught F. Namavar D. M. MacLaren

Pathogenesis

Escherichia

10.1007/bf00404584

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Comparison of monoclonal antibodies to the deeper core region of Gram-negative lipopolysaccharide by means of polymyxin B inhibition studies

Microbial Pathogenesis (1988)

Ben J. Appelmelk A. M. J. J. Verweij-Van Vught Janneke J. Maaskant W. F. Schouten L. G. Thijs

Polymyxin

Lipid A

Polymyxin B

Gram-Negative Bacteria

10.1016/0882-4010(88)90102-7

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Polymorphonuclear Leukocyte Chemotaxis by Mixed Anaerobic and Aerobic Bacteria

Journal of Medical Microbiology (1984)

F. Namavar A. M. J. J. Verweij-Van Vught W. A. C. Vel Munita Bal D. M. MacLaren

SUMMARY The induction of chemotactic activity of polymorphonuclear leukocytes (PMNL) by anaerobic and aerobic bacteria alone or in combination was evaluated. Washed cells as well as the supernate of Proteus mirabilis were chemotactic for leukocytes. The supernate of cultures of two strains of Bacteroides fragilis contained small amounts of chemotactic factors. No chemotactic factors were released from the non-fragilis Bacteroides strains. The supernates of cultures of anaerobic bacteria were capable of inhibiting chemotaxis of leukocytes to the chemotactic factors of P. mirabilis. P. mirabilis and two strains of B. fragilis generated chemotactic factors in serum but none of the other Bacteroides spp. tested were able to induce serum chemotactic factors.

Bacteroides fragilis

Bacteroidaceae

10.1099/00222615-18-2-167

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Citations (30)

The effect of antimicrobial agents on the killing of Escherichia coli in the presence of Bacteroides fragilis

Antonie van Leeuwenhoek (1985)

F. Namavar A. M. J. J. Verweij-Van Vught W. A. C. Vel D. M. MacLaren

Bacteroides fragilis

10.1007/bf00404597

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Comparison of biochemical and serological typing results and antimicrobial susceptibility patterns in the epidemiological investigation of Klebsiella spp

Epidemiology and Infection (1987)

A. M. Simoons‐Smit A. M. J. J. Verweij-Van Vught P. M. J. M. de Vries D. M. MacLaren

SUMMARY An analysis of the serological and biochemical typing results of 925 clinical isolates of klebsiella revealed that biotyping and serotyping of klebsiella could replace each other for epidemiological purposes. The combination of both typing methods provided even more epidemiological information in analysing clusters of particular serotypes and biotypes in time. Clustering serotypes, mainly of neonatal origin, were nearly uniformly more resistant to the antibiotics in common use than other serotypes. Biotyping as well as serotyping of klebsiella isolates recovered from environmental surveys in the neonatal ward showed that epidemic and non-epidemic klebsiella isolates could occasionally be cultured from the environment and from the staff.

Klebsiella

Phage typing

10.1017/s0950268800066474

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Citations (1)

An enzyme-linked immunosorbent assay (ELISA) for the measurement of antibodies to different parts of the gram-negative lipopolysaccharide core region

Journal of Immunological Methods (1985)

Ben J. Appelmelk A. M. J. J. Verweij-Van Vught D. M. MacLaren L. G. Thijs

Bovine serum albumin

Lipid A

10.1016/0022-1759(85)90351-5

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Citations (37)