Wan-Lin Wu

National Cheng Kung University

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Yu‐Yun Hsiao

National Cheng Kung University

Wen‐Chieh Tsai

National Cheng Kung University

Zhong‐Jian Liu

Fujian Agriculture and Forestry University

You‐Yi Chen

National Chiayi University

Nobutaka Mitsuda

National Institute of Advanced Industrial Science and Technology

Zhi-Wen Wang

East China University of Science and Technology

Yves Van de Peer

Ghent University

Ke-Wei Liu

Tsinghua University

Shan-Ce Niu

Hebei Agricultural University

Chuan‐Ming Yeh

National Chung Hsing University

Cooperative Institutions

National Cheng Kung University

National Defense Medical Center

Fujian Agriculture and Forestry University

National Taiwan University

Ningxia Academy of Agriculture and Forestry Sciences

Tri-Service General Hospital

Chinese Academy of Sciences

National Yang Ming Chiao Tung University

Academia Sinica

State Forestry and Grassland Administration

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The Dendrobium catenatum Lindl. genome sequence provides insights into polysaccharide synthase, floral development and adaptive evolution

Scientific Reports (2016)

Guoqiang Zhang Qing Xu Chao Bian Wen‐Chieh Tsai Chuan‐Ming Yeh

Orchids make up about 10% of all seed plant species, have great economical value, and are of specific scientific interest because of their renowned flowers and ecological adaptations. Here, we report the first draft genome sequence of a lithophytic orchid, Dendrobium catenatum. We predict 28,910 protein-coding genes, and find evidence of a whole genome duplication shared with Phalaenopsis. We observed the expansion of many resistance-related genes, suggesting a powerful immune system responsible for adaptation to a wide range of ecological niches. We also discovered extensive duplication of genes involved in glucomannan synthase activities, likely related to the synthesis of medicinal polysaccharides. Expansion of MADS-box gene clades ANR1, StMADS11, and MIKC(*), involved in the regulation of development and growth, suggests that these expansions are associated with the astonishing diversity of plant architecture in the genus Dendrobium. On the contrary, members of the type I MADS box gene family are missing, which might explain the loss of the endospermous seed. The findings reported here will be important for future studies into polysaccharide synthesis, adaptations to diverse environments and flower architecture of Orchidaceae.

Dendrobium

Orchidaceae

10.1038/srep19029

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Citations (301)

Advanced glycation end products cause collagen II reduction by activating Janus kinase/signal transducer and activator of transcription 3 pathway in porcine chondrocytes

Lara D. Veeken (2011)

Ching‐Yu Huang K.-Y. Lai Li-Feng Hung Wan-Lin Wu Fuchen Liu

Objectives. The major risk factor for OA is ageing; however, the mechanisms remain largely unclear. We investigated the effects and mechanisms of advanced glycation end products (AGEs) that accumulate in aged joints in chondrocytes. Methods. Porcine chondrocytes or cartilage fragments were prepared. Gene expression of MMPs and a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) was assessed by real-time RT–PCR. Gelatin zymography was used to determine MMP-13 enzyme activity. Histochemistry or immunoblotting analysis was applied to determine the expression of collagen II, proteoglycan and aggrecan. Electrophoretic mobility shift assay and immunoblotting were used to study the activation of signal transducer and activator of transcription 3 (STAT3). Genetic manipulations with short hairpin RNA (shRNA) or dominant negative constructs were applied. Results. AGE enhanced expression and enzyme activity of MMP and ADAMTS genes and resulted in reduction of collagen II. Both janus kinase 2 (JAK2) and JAK3 inhibitors suppressed AGE-induced MMP-13, ADAMTS-4 and ADAMTS-5 expression and enzyme activity. Inhibition of JAK2 or JAK3 prevented AGE-mediated decrease of collagen II in chondrocytes and proteoglycan (aggrecan) degradation in cartilage fragments. In addition, interference of STAT3 expression inhibited AGE-induced MMP-13 and ADAMTS enzyme activities and mRNA levels. Furthermore, expression of the dominant negative receptor of AGE (DN-RAGE) blocked AGE-induced STAT3 phosphorylation. Conclusion. Blocking JAK/STAT3 signalling pathway inhibited AGE-induced activation of MMP-13 and ADAMTS and prevented AGE-mediated decrease of collagen II and proteoglycan (aggrecan). The results indicated that JAK/STAT3 pathway may be a potential target for designing disease-modifying drugs for the treatment of OA.

Aggrecan

ADAMTS

10.1093/rheumatology/ker134

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Citations (71)

Epigallocatechin-3-gallate Reduces Scavenger Receptor A Expression and Foam Cell Formation in Human Macrophages

Journal of Agricultural and Food Chemistry (2017)

Sy‐Jou Chen Yung-Hsi Kao Jing Li Yi-Ping Chuang Wan-Lin Wu

Foam cells are formed when macrophages imbibe low-density lipoprotein (LDL) through scavenger receptors. Here we examined how epigallocatechin-3-gallate (EGCG) influences foam cell formation. We found that EGCG dose-dependently reduced oxidized LDL (oxLDL) uptake in THP-1 (10 μM, 20.0 ± 0.50, p < 0.05) and primary macrophages (134.6 ± 15.6, p < 0.05) and reduced intracellular cholesterol content in these cells, respectively (10 μM, 32.6 ± 0.14, p < 0.05; 31.7 ± 1.26, p < 0.05). EGCG treatment decreased scavenger receptor A expression, but not the expression of CD36 or of reverse cholesterol transporters. Moreover, EGCG stimulated translocation of the p50 and p65 subunits of NF-κB and enhanced NF-κB DNA-binding activity, thus suppressing SR-A promoter activity. EGCG's suppression of SR-A expression was blocked by the NF-κB inhibitor Bay. The present findings suggest that EGCG regulates NF-κB activity and thus suppresses SR-A expression, oxLDL uptake, and foam cell formation.

Foam cell

Scavenger Receptor

Scavenger

Gallate

10.1021/acs.jafc.6b05832

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Citations (24)

PKCδ signalling regulates SR-A and CD36 expression and foam cell formation

Cardiovascular Research (2012)

Chin‐Sheng Lin Feng‐Yen Lin Ling‐Jun Ho Chien‐Sung Tsai Shu-Mung Cheng

AimsThe formation of foam cells is crucial in the initiation and progression of atherosclerosis. One of the critical steps in foam cell formation is the uptake of low-density lipoprotein (LDL) by macrophages via scavenger receptors (SRs). This study examined the role of protein kinase C (PKC) isoforms on foam cell formation.

Foam cell

CD36

Rottlerin

10.1093/cvr/cvs189

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Citations (63)

Mobility of Polymer Chains Confined at a Free Surface

Physical Review Letters (2001)

Yong‐Jin Pu M. H. Rafailovich Jonathan Sokolov Dilip Gersappe T. C. Peterson

Dynamic secondary ion mass spectrometry was used to investigate the chain mobility of polystyrene (MW ranging from 4.3 to 957 kg/mol) at the free surface. The data show that the diffusion coefficient was reduced relative to the bulk value within a distance, $d\ensuremath{\le}{4R}_{g}$, from the surface and scaled as ${1/N}^{2.5}$ at fixed d. These results are in excellent agreement with self-consistent field calculations of the surface segmental distribution and provide the first direct confirmation of various theoretical models that predict asymmetric segmental fluctuation which arises from surface induced orientation of polymer chains.

Polystyrene

Free surface

Chain (unit)

10.1103/physrevlett.87.206101

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Citations (71)

P-35: New Voltage-Programmed AMOLED Pixel Circuit Employing In-Pixel Compensation Scheme for Mobility Variation

SID Symposium Digest of Technical Papers (2016)

Chih‐Lung Lin Fu-Hsing Chen Yen‐Ting Liu Chun-Ming Lu Wan-Lin Wu

This work proposes a new active-matrix organic light-emitting diode (AMOLED) pixel circuit using an in-pixel mobility compensation technique. Moreover, the circuit can compensate for both power line voltage drop and threshold voltage variation. Thus, it is suited for backplanes utilizing p-type low-temperature polycrystalline-silicon thin-film transistors (LTPS TFTs). Simulated results demonstrate that the compensation functions are succeed at a Full HD (1920×1080) resolution with a 60 Hz frame rate. The current error rates of power line voltage drop and threshold voltage variation are all below 3%, and those of mobility variation are below 6.5%.

AMOLED

Overdrive voltage

Voltage drop

10.1002/sdtp.10872

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Citations (6)

C- and D-class MADS-Box Genes from Phalaenopsis equestris (Orchidaceae) Display Functions in Gynostemium and Ovule Development

Plant and Cell Physiology (2012)

You‐Yi Chen Pei-Fang Lee Yu‐Yun Hsiao Wan-Lin Wu Zhao-Jun Pan

Gynostemium and ovule development in orchid are unique developmental processes in the plant kingdom. Characterization of C- and D-class MADS-box genes could help reveal the molecular mechanisms underlying gynostemium and ovule development in orchids. In this study, we isolated and characterized a C- and a D-class gene, PeMADS1 and PeMADS7, respectively, from Phalaenopsis equestris. These two genes showed parallel spatial and temporal expression profiles, which suggests their cooperation in gynostemium and ovule development. Furthermore, only PeMADS1 was ectopically expressed in the petals of the gylp (gynostemium-like petal) mutant, whose petals were transformed into gynostemium-like structures. Protein–protein interaction analyses revealed that neither PeMADS1 and PeMADS7 could form a homodimer or a heterodimer. An E-class protein was needed to bridge the interaction between these two proteins. A complementation test revealed that PeMADS1 could rescue the phenotype of the AG mutant. Overexpression of PeMADS7 in Arabidopsis caused typical phenotypes of the D-class gene family. Together, these results indicated that both C-class PeMADS1 and D-class PeMADS7 play important roles in orchid gynostemium and ovule development.

MADS-box

Petal

Phalaenopsis

10.1093/pcp/pcs048

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Citations (56)

Diffusion of linear polystyrene into crosslinked polystyrene.

Macromolecules (2003)

Wan-Lin Wu W.E. Wallace John H. van Zanten Apollo P. Y. Wong Ipns

Polystyrene

Linear polymer

Source

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Citations (0)

Acidic-rich region of amyloid precursor protein induces glial cell apoptosis

APOPTOSIS (2004)

Kai Sun G.-H. SUN Yi‐Chang Su Chung‐I Chang Mei-Jan Chuang

Jurkat cells

10.1023/b:appt.0000045793.44842.e7

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Citations (4)

Probe Diffusion in Thin PS Free-Standing Films

Macromolecules (2001)

Yong‐Jin Pu Hilary White Miriam Rafailovich Konstantin Sokolov Ajay M. Patel

Dynamic secondary ion mass spectrometry was performed to determine the dynamics of thin free-standing polystyrene films by investigating probe diffusion in a high molecular weight polymer matrix. It is found that the temperature dependence of the diffusion of small molecular probes can be described by the WLF equation above bulk Tg, whereas a weaker dependence is found below Tg for PS films with total thickness as thin as 69 nm. The results of probe diffusion as a function of film thicknesses show no change of Tg for the free-standing films with thickness ranging from 33 to 200 nm.

Polystyrene

Self-diffusion

10.1021/ma002183f

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Citations (37)