To understand better the immunopathology of HTLV-I uveitis by investigating the clonality of HTLV-I-infected T-cell clones.Eleven T-cell clones were established from the aqueous humor (six clones) and the peripheral blood (five clones) of a patient with HTLV-I uveitis, and the clonality of the HTLV-I-infected T cells was investigated by sequencing the T-cell receptor (TCR) alpha gene after the amplification of TCR alpha cDNA using an adaptor-ligation method and reverse transcriptase-polymerase chain reaction (RT-PCR).TCR alpha use was different for each of 11 T-cell clones, encompassing eight different HTLV-I-infected T-cell clones (four from the aqueous humor and four from peripheral blood) and three HTLV-I-negative T-cell clones.This study demonstrated polyclonal use of TCR alpha for HTLV-I-infected T cells in the ocular lesion and the peripheral blood. Results suggested that these T cells are not precursors of the leukemic cells associated with malignant transformation. Instead, they might be randomly infected with HTLV-I in the process of HTLV-I uveitis.
A severe, extensive, erosive stomatitis developed in a 33-year-old Japanese man who had had intraperitoneal giant lymph node hyperplasia (Castleman’s tumor) of the hyaline-vascular type. Histologically, there was a dense lymphoid cell infiltration in the dermis with invasion of the mucosal epithelium. It was hypothesized that an autoaggressive attack of lymphoid cells against the epithelium of the oral mucosa played a role in the pathogenesis of this erosive stomatitis and it was suggested that there might be a correlation between the occurrence of stomatitis and the presence of Castleman’s tumor.
重篤な非溶血性輸血後副作用であるアナフィラキシーショックのリスク因子であるハプトグロビン欠損症の原因遺伝子はハプトグロビン遺伝子欠失（HPdel）であり，その頻度は約4,000人に1人であると予想される．我々は最近安全な輸血医療の遂行を目的とし迅速・簡便なリアルタイムPCR法に基づく2種のHPdel診断法を開発し報告した．今回，臨床現場への導入を目的として久留米大学病院で輸血予定患者の血液を鋳型とし，TaqMan probeを用いる方法とSYBR green Iを用い融解曲線をおこなう方法の2法を実施し結果を比較した．約1時間半で得られた結果は全サンプルで一致し，2009年1月から2010年3月末に解析した2,954名のうち91名がHP/HPdel，1名がHPdel/HPdelであった．TaqMan法は増幅シグナルそのものが結果を反映することから反応中に診断結果を予想でき，HPdel/HPdelがソフトウエアで自動検出できるため多検体処理能力に優れた方法であり，SYBR法は初期費用が低く抑えられ，より幅広い臨床現場での導入が容易であると考えられた．
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