Journal Article High EDTA concentrations cause entrapment of small DNA molecules in the compression zone of pulsed field gels, resulting in smaller than expected insert sizes in YACs prepared from size selected DNA Get access Roel P. Funke, Roel P. Funke * Plant Molecular Genetics and Center for Legume Research, Institute of Agriculture, The University of TennesseeKnoxville, TN 37901-1071, USA * To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Alexander Kolchinsky, Alexander Kolchinsky Plant Molecular Genetics and Center for Legume Research, Institute of Agriculture, The University of TennesseeKnoxville, TN 37901-1071, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Peter M. Gresshoff Peter M. Gresshoff Plant Molecular Genetics and Center for Legume Research, Institute of Agriculture, The University of TennesseeKnoxville, TN 37901-1071, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 22, Issue 13, 11 July 1994, Pages 2708–2709, https://doi.org/10.1093/nar/22.13.2708 Published: 11 July 1994 Article history Received: 05 May 1994 Accepted: 03 June 1994 Published: 11 July 1994
We constructed a BAC library of the model legume Lotus japonicus with a 6-to 7-fold genome coverage. We used vector PCLD04541, which allows direct plant transformation by BACs. The average insert size is 94 kb. Clones were stable in Escherichia coli and Agrobacterium tumefaciens.