Donald W. Seldin

The University of Texas Southwestern Medical Center

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Floyd C. Rector

University of California, San Francisco

Gerhard Giebisch

Yale University

F. C. Rector

The University of Texas Southwestern Medical Center

Robert J. Alpern

Yale University

Juha P. Kokko

National Defence University

Norman W. Carter

The University of Texas Southwestern Medical Center

Orson W. Moe

The University of Texas Southwestern Medical Center

Wadi N. Suki

Houston Methodist

Garabed Eknoyan

Baylor College of Medicine

Roland C. Blantz

University of California, San Diego

Cooperative Institutions

The University of Texas Southwestern Medical Center

Yale University

Harvard University

RELX Group (United States)

United States Department of Veterans Affairs

University of Zurich

University of California, Los Angeles

Northwestern University

Southwestern Medical Center

Baylor College of Medicine

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AN ANALYSIS OF THE MECHANISM OF THE INHIBITORY INFLUENCE OF K+ ON RENAL H+ SECRETION*

Journal of Clinical Investigation (1962)

Floyd C. Rector Harold E. Buttram Donald W. Seldin

10.1172/jci104516

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Citations (9)

EVIDENCE FOR A DIRECT EFFECT OF SERUM SODIUM CONCENTRATION ON SODIUM REABSORPTION*

Journal of Clinical Investigation (1962)

Carl Goldsmith Floyd C. Rector Donald W. Seldin

Renal sodium reabsorption

10.1172/jci104542

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Citations (36)

Influence of unreabsorbed anions on renal threshold and Tm for bicarbonate

American Journal of Physiology-Legacy Content (1962)

Floyd C. Rector Donald W. Seldin

The relation of HCO 3 – reabsorption to excretion was examined in dogs with normal pCO 2 , increased plasma pCO 2 , and during the administration of acetazolamide. In the presence of an intact carbonic anhydrase enzyme system HCO 3 – excretion began when HCO 3 – reabsorption exceeded 75% of the HCO 3 – reabsorptive capacity. Respiratory acidosis augments the HCO 3 – Tm without altering the pattern of HCO 3 – excretion which commences at near-capacity levels. Inhibition of carbonic anhydrase by acetazolamide lowers the HCO 3 – Tm, enormously exaggerates the HCO 3 – leak, and prevents the production of HCO 3 – -free urine at all levels of plasma HCO 3 – . Sodium sulfate markedly diminishes the excretion of HCO 3 – before the Tm is reached, both in the presence or absence of carbonic anhydrase activity, but has no effect on the HCO 3 – Tm, suggesting that the presence of unreabsorbed anion influences the ability of renal tubules to generate a gradient without influencing total H + transport. It is suggested that SO 4 – can induce this effect because it is an unreabsorbed anion. Reasons are advanced that the anion effect occurs in the distal, not proximal, tubule and is mediated by a rise in transtubular potential.

Acetazolamide

Bicarbonate

Renal physiology

Renal sodium reabsorption

Tubular fluid

Carbonic acid

10.1152/ajplegacy.1962.202.2.313

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Citations (14)

Demonstration of a hormonal inhibitor of proximal tubular reabsorption during expansion of extracellular volume with isotonic saline

Journal of Clinical Investigation (1968)

Floyd C. Rector Manuel Martínez–Maldonado Neil A. Kurtzman Jack C. Sellman Fred Oerther

Evidence for the elaboration of a hormonal inhibitor of renal tubular reabsorption in response to expansion of extracellular fluid volume was obtained by examining the effects of plasma from rats and dogs undergoing saline diuresis on the rate of proximal tubular reabsorption measured both directly by micropuncture techniques and indirectly by clearance techniques.

Isotonic saline

Isotonic Solutions

Renal sodium reabsorption

Renal physiology

Volume expansion

Tubular fluid

10.1172/jci105771

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Citations (69)

The Kidney Physiology and Pathophysiology. Vol.2

Donald W. Seldin Gerhard Giebisch

Pathophysiology

Source

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Citations (24)

Preface

Kidney International (2004)

Donald W. Seldin

Nobel laureate

Excellence

Tribute

10.1111/j.1523-1755.2004.09101.x

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Citations (0)

In Memoriam

Journal of the American Society of Nephrology (2004)

C. Brun Hugh E. deWardener Priscilla Kincaid‐Smith George E. Schreiner G Richet

Abstract

Lipid-based carriers of the hydrophobic drug paclitaxel (PTX) are used in clinical trials as next-generation agents for cancer chemotherapy. Improving the loading capacity of these carriers requires enhanced PTX solubilization. We compared the solubility of PTX in cationic liposomes (CLs) with lipid tails containing one (oleoyl; C18:1 Δ⁹; DOTAP/DOPC) or two (linoleoyl; C18:2 Δ⁹; DLinTAP/DLinPC) cis double bonds with newly synthesized cationic DLinTAP (2,3-dilinoleoyloxypropyltrimethylammonium methylsufate). We used differential-interference-contrast microscopy to directly observe PTX crystal formation and generate kinetic phase diagrams representing the time-dependence of PTX solubility as a function of PTX content in the membrane. Replacing tails bearing one cis double bond (DO lipids) with those bearing two (DLin lipids) significantly increased PTX membrane solubility in CLs. Remarkably, 8 mol% PTX in DLinTAP/DLinPC CLs remained soluble for approximately as long as 3 mol% PTX (the membrane solubility limit which has been the focus of most previous fundamental studies and clinical trials) in DOTAP/DOPC CLs. The large increase in solubility is likely caused by enhanced molecular affinity between lipid tails and PTX upon replacement of oleoyl by linoleoyl tails, rather than by the transition in membrane structure from lipid bilayers to inverse cylindrical micelles observed in small-angle X-ray scattering. Importantly, the efficacy of PTX-loaded CLs against human prostate cancer (PC3) cells from measurements of the IC50 of PTX cytotoxicity was unaffected by changing the lipid tails, and toxicity of the CL carrier alone was negligible. Moreover, efficacy was approximately doubled against human melanoma (M21) cells for PTX-loaded DLinTAP/DLinPC over DOTAP/DOPC CLs. The findings demonstrate the potential of chemical modifications of the lipid tails to increase the PTX membrane loading well over the typically used 3 mol% while maintaining (and in some cases even increasing) the efficacy of CLs. The increased PTX solubility will aid the development of liposomal PTX carriers that require significantly less lipid to deliver a given amount of PTX, reducing side effects and costs.

Lipophilicity

Cationic polymerization

10.1097/01.asn.0000147146.63311.37

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Examination of the mixing hypothesis as an explanation for elevated urinary CO₂ tensions

American Journal of Physiology-Legacy Content (1959)

Floyd C. Rector Richard M. Portwood Donald W. Seldin

To examine the adequacy of the mixing hypothesis as an explanation for high urinary CO 2 tensions, urine-plasma pCO 2 gradients were examined in normal subjects receiving NaHCO 3 infusions in whom urinary buffer concentration was minimized by solute and water diuresis, antecedent carbohydrate diet and glucose infusions. U-P gradients varying from 23 to 78 mm Hg were generated in the face of minimal urinary buffer concentrations which varied from 1.40 to 2.95 mEq/l. Only by the most extreme assumptions, i. e. maximal heterogeneity and no back-diffusion of CO 2 , could the mixing hypothesis account for the observed gradients. Since these assumptions were highly improbable, it was concluded that the mixing hypothesis is not a tenable explanation of the high CO 2 tension of alkaline urine.

10.1152/ajplegacy.1959.197.4.861

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Citations (14)

The generation and maintenance of metabolic alkalosis

Kidney International (1972)

Donald W. Seldin Floyd C. Rector

Alkalosis

10.1038/ki.1972.43

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Citations (164)

Donald Wayne Seldin, MD: A Conversation with the Editor

Baylor University Medical Center Proceedings (2003)

Donald W. Seldin

10.1080/08998280.2003.11927905

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Citations (3)