G. Marbaix

Université Libre de Bruxelles

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Role of the Polyadenylate Segment in the Translation of Globin Messenger RNA in Xenopus Oocytes

Proceedings of the National Academy of Sciences (1974)

G. Huez G. Marbaix E. Hubert M Leclercq Uri Nudel

The translations of native messenger RNA for rabbit globin and that of poly(A)-free globin messenger RNA have been compared after injection into Xenopus oocytes. The initial rate of translation of poly(A)-free mRNA is close to that found with intact mRNA. However, at longer incubation periods, the rate of globin synthesis with poly(A)-free mRNA is considerably lower than with native mRNA. Similar differences in the template activity of the two mRNA preparations were found with a cell-free extract of Krebs II ascites tumor. It is concluded that the presence of the 3' poly(A)-rich sequence in mRNA is required to ensure high functional stability.

10.1073/pnas.71.8.3143

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Citations (148)

LIBERATION OF A COMPLEX CONTAINING mRNA PROM RETICULOCYTE POLYRIBOSOMES

De Gruyter eBooks (1968)

Arsène Burny G. Marbaix G. Huez

Reticulocyte

Polysome

Liberation

Prom

10.1515/9783112748831-047

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Préparation de RNA Messager de Réticulocytes de Lapin par Ultracentrifugation en Rotor Zonal

European Journal of Biochemistry (1967)

G. Huez Arsène Burny G. Marbaix E. Schram

Two methods are described for the rapid isolation of 0.6 mg to 1 mg of 9S RNA from rabbit reticulocytes. This RNA has properties expected for messenger RNA. In the first procedure, reticulocyte polyribosomes are dissolved in 0.5% (w/v) sodium dodecylsulfate and messenger RNA is separated by sucrose gradient centrifugation in a zonal rotor (B IV‐Spinco). In the second method, messenger RNA is detached from polyribosomes by EDTA (3x10 −2 M) treatment and separated from ribosomal sub‐particles by sucrose gradient centrifugation using the same rotor. In both cases, messenger RNA is then concentrated by adsorption on a non‐ionic cellulose column. Further purification may be obtained by sucrose gradient centrifugations using the rotor S. W. 25.2 (Spinco).

Sucrose gradient

Polysome

Reticulocyte

10.1111/j.1432-1033.1967.tb00060.x

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Citations (7)

Bovine Leukemia

CRC Press eBooks (2024)

A. Bumy Y. Cleuter R. Kettmann M. Mammerickx G. Marbaix

Bovine leukemia virus (BLV) is the etiological agent of a chronic lymphatic leukemia/lymphoma in cows, sheep, and goats. Infection without neoplastic transformation was also obtained in pigs, rhesus monkeys, chimpanzees, and rabbits and observed in capybaras and water buffalo. Structurally and functionally, BLV is a relative of human T-lymphotropic viruses 1 and 2 (HTLV-I and HTLV-II). HTLV-I induces in humans a T-cell leukemia, and its type II counterpart has been found in dermatopathic lymphadenopathy, hairy T-ceU leukemia, and prolymphocytic leukemia cases. At variance with HTLV-I, BLV has not been associated with neurological diseases of the degenerative type.

10.1201/9781003573708-2

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Enzootic Bovine Leukosis and Bovine Leukemia Virus

Springer eBooks (1983)

G. Marbaix R. Kettmann Jacqueline Deschamps Dominique Couez M. Mammerickx

Bovine leukemia virus

Enzootic

Leucosis

Lymphocytosis

Cattle Diseases

10.1007/978-1-4684-4541-1_11