A recent genetic and physical study (Arber et al. 1979) of spontaneous P1 prophage mutants affected in the vegetative phage propagation revealed that at the most a few percent of spontaneous mutations were caused by nucleotide substitution or other small alterations in the nucleotide sequence. About 70% of all mutations studied were relatively long deletions clustering around the single IS1 element carried in the wild-type P1 genome. The rest, about 27% of the mutants, carried an additional DNA segment most likely representing a transposable IS element from the Escherichia coli host chromosome rather than a partial duplication of phage genetic material. On the one hand, these findings point to the importance of IS elements in the production of spontaneous mutations. On the other hand, they open an interesting approach to investigate the diversity of IS elements carried in bacteria serving as hosts for bacteriophage P1.
Journal Article Floral tissue of Petunia hybrida (V30) expresses only one member of the chalcone synthase multigene family Get access R.E. Koes, R.E. Koes 1Department of Applied Genetics, Free UniversityDe Boelelaan 1087, 1081HV Amsterdam, The Metherlands Search for other works by this author on: Oxford Academic PubMed Google Scholar C.E. Spelt, C.E. Spelt 1Department of Applied Genetics, Free UniversityDe Boelelaan 1087, 1081HV Amsterdam, The Metherlands Search for other works by this author on: Oxford Academic PubMed Google Scholar H.J. Reif, H.J. Reif 2Bayer A.G., PF-ABF/Biotechnologie, D 5090 Leverkusen, FRG Search for other works by this author on: Oxford Academic PubMed Google Scholar P.J.M. van den Elzen, P.J.M. van den Elzen 1Department of Applied Genetics, Free UniversityDe Boelelaan 1087, 1081HV Amsterdam, The Metherlands3MOGEN Int, B.V. Niels Bohrweg 11-13, 2333 CA Leiden, The Netherlands Search for other works by this author on: Oxford Academic PubMed Google Scholar E. Veltkamp, E. Veltkamp 1Department of Applied Genetics, Free UniversityDe Boelelaan 1087, 1081HV Amsterdam, The Metherlands Search for other works by this author on: Oxford Academic PubMed Google Scholar J.N.M. Mol J.N.M. Mol * 1Department of Applied Genetics, Free UniversityDe Boelelaan 1087, 1081HV Amsterdam, The Metherlands *To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 14, Issue 13, 11 July 1986, Pages 5229–5239, https://doi.org/10.1093/nar/14.13.5229 Published: 11 July 1986 Article history Received: 04 April 1986 Revision received: 02 June 1986 Accepted: 02 June 1986 Published: 11 July 1986
