In recent studies, sodium alginate (SA) has mainly been a plant growth promoter for many plants and crops as a novel bio stimulating materials for increasing plant growth under various conditions. Pot testing was done to examine the impacts of various levels of SA (25, 50 and 100 ppm) on the growth and physiological activities of Corchorus olitorius (molokhya), Vicia faba (faba bean) and Zea mays (corn) plants in two vegetative stages after 10 and 40 days from sowing. Treatment with different SA concentrations improved significantly the physiological characters of the used plants. The data indicated that the used SA concentrations increased photosynthetic pigments, carbohydrate contents, nitrogen contents, antioxidant enzymes and overall protein significantly as compared to control values.
Different bacterial strains were recorded as onion rot pathogens. The candidate strains in one region may differ from the other and it may be only one pathogen responsible of the infection in an area or a combination from different pathogens. The present research aimed at detection of the main bacterial pathogen (s) causing onion rot in Egypt throughout storage. Five bacterial pathogens were detected with different occurrence percentage; Erwinia carotovora subspecies carotovora (48.14 %), Erwinia cacticida (18.51 %), Erwinia carotovora subspecies atroseptica (14.81 %), Burkholderia cepacia (14.81 %) and Pantoea sp. (3.7 %).
The utilization of cuttings remains a prevalent method for vegetative propagation in various plant species. Synthetic rooting hormones have conventionally been employed to enhance the rooting process; however, their high cost and potential environmental risks have necessitated the exploration of natural alternative compounds. In this study, the efficacy of natural plant extracts (pure honey, aloe extract, willow extract, moringa extract, and cinnamon powder) and synthetic auxin derivatives (indole-3-butyric acid and α-naphthaleneacetic acid) at a concentration of 2000 ppm was compared for their influence on rooting and vegetative growth of snowbush (Breynia disticha) cuttings, a significant ornamental and medicinal shrub. Results demonstrated that while the synthetic hormones produced the highest survival percentages and root number, the roots were consistently short, thick, friable, and largely void of secondary roots. Conversely, the alternative natural plant extracts, particularly aloe extract, exhibited promising outcomes, showcasing good rooting percentage and root number, significantly increased root length, and fresh and dry root weight. Furthermore, aloe extract demonstrated the potential to enhance shoot length and alter the chemical constituents of the cuttings. The highest values of total phenols and nitrogen percentage were recorded with cuttings treated with indole-3-butyric acid, followed by cuttings treated with aloe extract. While using aloe extract, we recorded the highest values of total carbohydrates, phosphorus, and potassium percentages. Notably, the highest endogenous levels of indole acetic acid and gibberellic acid, along with the lowest abscisic acid concentration in cutting tissues, were associated with the highest rooting percentage observed with indole-3-butyric acid, followed by the use of aloe extract. We hypothesized that natural plant extracts, specifically aloe extract, have potential as a viable alternative for synthetic auxins in promoting successful rooting and vegetative growth in snowbush cuttings. Further research is warranted to explore the underlying mechanisms and optimize the application of these natural compounds in vegetative propagation techniques.
Fungi able to degrade agriculture wastes were isolated from different soil samples, rice straw, and compost; these isolates were screened for their ability to produce β-glucosidase. The most active fungal isolate was identified as Talaromyces pinophilus strain EMOO 13-3. The Plackett-Burman design is used for identifying the significant variables that influence β-glucosidase production under solid-state fermentation. Fifteen variables were examined for their significances on the production of β-glucosidase in 20 experimental runs. Among the variables screened, moisture content, Tween 80, and (NH4)2SO4 had significant effects on β-glucosidase production with confidence levels above 90% (p < 0.1). The optimal levels of these variables were further optimized using Box-Behnken statical design. As a result, the maximal β-glucosidase activity is 3648.519 U g(-1), which is achieved at the following fermentation conditions: substrate amount 0.5 (g/250 mL flask), NaNO3 0.5 (%), KH2PO4 0.3 (%), KCl 0.02 (%), MgSO4 · 7H2O 0.01 (%), CaCl2 0.01 (%), yeast extract 0.07 (%), FeSO4 · 7H2O 0.0002 (%), Tween 80 0.02 (%), (NH4)2SO4 0.3 (%), pH 6.5, temperature 25°C, moisture content 1 (mL/g dry substrate), inoculum size 0.5 (mL/g dry substrate), and incubation period 5 days.
A foliar application of Atonik (250, 500 and 1000 ppm) and benzyl adenine (25, 50 and 100 ppm) under vernalization was investigated on Solanum lycopersicum (var. Beto 86) plant. All determined growth parameters (root length, root fresh and weights, shoot length, number of leaves, number of nodes, total leaf area, shoot fresh and dry weights and relative water content) were inhibited in response to treatment with vernalization. Meanwhile vernalization in combination with varying concentration of Atonik or benzyl adenine (BA) led to a general significant increase in these parameters. Vernalization alone or in combination with Atonik or benzyl adenine accelerated flowering especially in response to 1000 ppm Atonik or 50 ppm BA under vernalization treatment. Vernalization treatment significantly increased the concentration of chlorophyll a and b, carotenoids and consequently total pigments. While chlorophyll a/b is insignificantly affected. In general, vernalization and different concentrations of Atonik or BA led to a massive increase in these pigments. Glucose, sucrose, polysaccharides, ammonia, amino and soluble as well as total nitrogen and protein were increased in tomato plants under the influence of vernalization alone or in combination with Atonik or BA. Moreover, a general significant increase in the content of K+, Na+ and Ca++, were detected in both tomato root and shoot as a result of treatments. Vernalization caused a significant decrease in total auxins, gibberellic acid and different cytokinin fractions in the shoot of tomato plants, whereas abscisic acid increased significantly by this treatment. At vernalization, all concentrations of Atonik or BA reverse this situation as compared with control values.
Key words: Atonik, benzyl adenine, Solanum lycopersicum, vernalization.
Fungi are considered one of the most important sources relied upon in the production of biodiesel, especially if the materials to be used are agricultural waste and are considered an inexpensive source of sugars. Aspergillus terreus Mekky221 was the most abundant of the Seven oleaginous fungal isolates that were isolated from Agriculture waste specimens were taken from cultivated land. and subsequently examined for biodiesel production in the current study. By using morphological (macroscopic and microscopic) analysis and molecular confirmation using 18S rRNA sequencing, the isolate's species identity was established and recorded on Gene Bank under accession number PQ182607. In order to enhance lipid accumulation, we also improved the culture conditions, Aspergillus terreus Mekky221 showed the highest lipid production (Dray biomass 4.89 ± 0.19 g/L giving lipid content 2.52 ± 0.06 g/L Equivalently 51.57 ± 3.03 %) in the existence of 100g/L sucrose and 10 g/L yeast extract at pH, 5 after five days of incubation at 30°C under static conditions. solid state fermentation medium was made: A 5-liter flask was filled with 40g of dry bagasse and rice straw as inexpensive carbon sources, mixture evenly moistened at a 60% level using sterile distilled water and peptone (5 g/1), autoclaved after cooling, spores added with known concentration, pH 5, temp. 30 and incubated for 5 days Aspergillus terreus Mekky221 showed lipid production (dray biomass 3.99 ± 0.17 g/L giving lipid content 2 .11 ± 0.08 g/L Equivalently 52.8 ± 0.13 %). Finally, the transesterification process is dependent on the one-step conversion of the fungal dry biomass's lipid to FAME and by using a gas chromatographic analysis of fatty acids. Aspergillus terreus Mekky221 is therefore thought to be a promising oleaginous filamentous fungus that can be used to biodiesel production from agricultural waste.