ABSTRACT Low temperatures are known to restrict chloroplast development and prevent the attainment of photosynthetic competence in maize leaves. The responses of the photosynthetic apparatus of mature maize leaves grown at 14°C on transfer of the plants to 25°C are examined. The synthesis of thylakoid proteins increased immediately on transfer of leaves from 14 to 25°C, with a dramatic accumulation of thylakoid proteins and chlorophylls occurring after 3 d at 25°C. Thylakoid structure and organization also became similar to those observed in leaves grown at 25°C over this period. However, no comparable development of photosynthetic competence in photosystems I and II or in the rate of CO 2 assimilation was observed on transfer of leaves from 14 to 25°C. Immunocytological analyses demonstrated heterogeneity in the distribution of a range of thylakoid proteins (cy tochrome f , the α and β subunits of the coupling factor, Dl of the photosytem II reaction centre, the 33kDa protein of the extrinsic oxygen‐evolving complex of photosystem II, and subunit II of photosystem I between mesophyll cells in leaves grown at 14°C, and in the responses of individual proteins to transfer of the leaves to 25°C. Such heterogeneity between mcsophyll cells would account for the inability of the leaves to develop the expected degree of photosynthetic competence on transfer to 25°C. The effects of low growth temperatures on chloroplast biogenesis are complex, as are the changes induced by the transfer ofleaves grown at low temperatures to optimal growth temperature, and both these factors may limit the canopy development and photosynthetic productivity of crops in temperate regions.
ABSTRACT Vitamin E (α‐tocopherol) is essential for the prevention of photo‐oxidative deterioration of biomembranes. The occurrence, relative biological activity and distribution of tocopherols in photosynthetic membranes is considered together with the possible biochemical and biophysical mechanisms by which tocopherols confer protection upon illuminated membranes. The common protective effects of Vitamin E in photo‐synthetic membranes and in medically important light‐induced diseases and conditions of the skin and eye in animal cell membranes are discussed. The importance of the Vitamin E‐Vitamin C thylakoid antioxidant system is also examined, in terms of susceptibility to photo‐oxidative damage under stress conditions including chilling, ageing and senescence, drought, atmospheric pollutants, herbicides and photosensitizing fungal toxins.
Explicit expressions are obtained for the bias, mean square, expected mean square and integrated mean square errors associated with the estimation of the normal probability density function using a Gaussian kernel. It is shown that this estimator is relatively robust to departures from normality and hence leads to satisfactory estimates under quite wide conditions. Two methods of bias reduction are suggested.
Lognormal Distributions: Theory and Applications (Statistics: Textbooks and Monographs Series, Vol. 88). Edited by E. L. Crow and K. Shimizu. ISBN 0 8247 7803 0. Dekker, New York, 1988. 408 pp. $79.75 (USA and Canada), $95.50 (elsewhere).