Abstract The distribution of various cell constituents of Bacillus megaterium has been studied in an aqueous two‐phase system containing the two polymers dextran and polythylene glycol. The difference in behaviour between cell walls, lipid granules and soluble cell material are such that a considerable fractionation of a suspension of disrupted cells may be obtained after a few counter‐current distributions. A mixture of whole cells and protoplasts can also be fractionated in this phase system. If sodium chloride is added to the phase system, vegetative cells and spores can be separated from each other.
1 An extracellular proteinase from a strain of Arthrobacter was coupled to cyanogen bromide activated agarose beads. 2 The coupled product was active at a fairly high temperature. No inactivation occurred upon incubation at 70°, where the free enzyme was rapidly inactivated. 3 No leakage of activity from the gel into solution was detectable under various conditions. 4 The mechanical properties of the activated and coupled product were superior to the untreated gel, both as regards flow rate and heat stability. No melting occurred up to 100°. 5 The use of proteolytic enzyme gels in columns allows the preparation of intermediate digestion products.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTProtease-catalyzed formation of plastein products and some of their propertiesBengt Van Hofsten and Georgios LalasidisCite this: J. Agric. Food Chem. 1976, 24, 3, 460–465Publication Date (Print):May 1, 1976Publication History Published online1 May 2002Published inissue 1 May 1976https://pubs.acs.org/doi/10.1021/jf60205a037https://doi.org/10.1021/jf60205a037research-articleACS PublicationsRequest reuse permissionsArticle Views111Altmetric-Citations39LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
