Obesity is said to be one of the exacerbating factors of chronic pain, however its mechanism is unclear. Recently, fatty acid binding protein 3 (FABP3) functions as not only an intracellular chaperone to transport fatty acids, but also the signal transduction and gene transcription. There are some recent reports that FABP3 is induced in response to an increased dietary fat load as well as obesity, inflammation and pain. Here we tested whether FABP3 involve in the mechanism for obesity-induced exacerbation of postoperative pain using FABP3 deficit (FABP3KO) mice. Male ddY and C57BL6J wild-type (WT) mice were used by experiment. WT and FABP3 KO were fed on control diet or high fat diet (HFD) for 8 weeks. Postoperative pain was induced by paw incision. Mechanical allodynia was evaluated by von Frey test. Mice with paw incision showed mechanical allodynia. Repeated intracerebroventricully injection of FABP-IN-1, a FABP inhibitor for FABP3, 5 and 7, suppressed paw incision-induced mechanical allodynia. The mice fed HF diet exacerbated paw incision-induced mechanical allodynia compared to those in control diet fed WT mice. On the other hand, FABP3KO mice fed HF diet suppressed paw incision-induced mechanical allodynia. Our findings suggest that FABP3 might at least in part involve in obesity-induced exacerbation of postoperative pain.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTResonance Raman spectra of ferrylporphyrins and related compounds in dioxygen matrixesLeonard M. Proniewicz, Krzysztof Bajdor, and Kazuo NakamotoCite this: J. Phys. Chem. 1986, 90, 9, 1760–1766Publication Date (Print):April 1, 1986Publication History Published online1 May 2002Published inissue 1 April 1986https://pubs.acs.org/doi/10.1021/j100400a007https://doi.org/10.1021/j100400a007research-articleACS PublicationsRequest reuse permissionsArticle Views215Altmetric-Citations54LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPlatinum(II)-olefin complexes containing amino acids. I. Preparation and structure of several platinum(II)- ethylene complexes with amino acidsKazuo Nakamoto, Junnosuke Fujita, and Kazuo KonyaCite this: Inorg. Chem. 1970, 9, 12, 2794–2796Publication Date (Print):December 1, 1970Publication History Published online1 May 2002Published inissue 1 December 1970https://pubs.acs.org/doi/10.1021/ic50094a038https://doi.org/10.1021/ic50094a038research-articleACS PublicationsRequest reuse permissionsArticle Views50Altmetric-Citations20LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Fatty acid-binding protein (FABP) regulates polyunsaturated fatty acids intracellular trafficking and functions as a signal transduction via modulation of gene expression. We have demonstrated that FABP3 protein was observed in microglia of the median eminence (ME) of hypothalamus and this protein was increased in the ME of pain model mice. These changes were correlated with the increment of hypothalamic docosahexaenoic acid (DHA) levels. Here, we assessed the effect of DHA on FABP3 expression using MG6 cell, a microglia cell line. Also, we tested the effect of FABP inhibitor on the mechanical allodynia in postoperative pain model mice. MG6 cells were cultured in Dulbecco's modified eagle medium with or without 10% fetal bovine serum (FBS) as a cell stress. FABP3 was measured by qPCR. Mechanical allodynia was assessed by von Frey test. FABP3 mRNA was expressed on the MG6 cell. Under the condition of serum-free media, FABP3 mRNA was also significantly increased compared to the media with 10% FBS. This increment was suppressed by DHA (300 μM). Repeated intraventricular injection of FABP inhibitor was significantly suppressed mechanical allodynia in postoperative pain mice. These results indicated that DHA might be involved in the regulation of microglial FABP3, and brain FABP might work as a regulator of pain.
Chronic stress has been shown to exacerbate pain conditions. However, the details mechanism remains unclear. Previously, we have demonstrated that docosahexaenoic acid (DHA), one of n-3 polyunsaturated fatty acids (PUFAs), suppressed pain through activation of G-protein-coupled receptor 40 (GPR40)/free fatty acid receptor (FFAR) 1. More recently, we found that repeated social defeat-stressed mice showed pain chronicity after paw-incision, and this pain chronicity was exacerbated by infusion of the GPR40/FFAR1 antagonist, GW1100 or GPR40/FFAR1 knockout mice. In the brain of this stressed mice with pain, the phosphatidylcholines with DHA and other PUFAs were remarkably decreased compared to non-stressed mice. Oral DHA supplementation was improved stress-induced pain chronicity. Furthermore, the n-3 fatty acid deficient mice showed exacerbation of pain after repeated stress. These results indicated that chronic stress may directly affect brain lipid composition, the related changes could be involved in chronic pain development. Our findings suggested that n-3 fatty acids, particularly DHA, are useful as a potential therapeutic target for stress-induced chronic pain. In this symposium, we would like to discuss the role of brain n-3 fatty acids-GPR40/FFAR1 signaling in stress-induced pain chronicity.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInfrared Spectra of Metallic Complexes. IV. Comparison of the Infrared Spectra of Unidentate and Bidentate Metallic ComplexesKazuo Nakamoto, Junnosuke Fujita, Shizuo Tanaka, and Masahisa KobayashiCite this: J. Am. Chem. Soc. 1957, 79, 18, 4904–4908Publication Date (Print):September 1, 1957Publication History Published online1 May 2002Published inissue 1 September 1957https://pubs.acs.org/doi/10.1021/ja01575a020https://doi.org/10.1021/ja01575a020research-articleACS PublicationsRequest reuse permissionsArticle Views2573Altmetric-Citations417LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
