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Pingli Xie

Central South University

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Guan‐Cheng Li

Gannan Medical University

Guohua Zhou

Nanjing General Hospital of Nanjing Military Command

Jinyue Hu

Central South University

Yuehui Li

Institute of Applied Ecology

Yuehui Li

Peking University

Guancheng Li

Central South University

Yongqing Tong

Wuhan University

Hui Xie

Xiangya Hospital Central South University

Yanhong Liu

Zhengzhou University

Zhen‐Xing Wang

Xiangya Hospital Central South University

Cooperative Institutions

Central South University

Xiangya Hospital Central South University

Sichuan University

Chinese Academy of Medical Sciences & Peking Union Medical College

Huazhong University of Science and Technology

Sun Yat-sen University

Second Xiangya Hospital of Central South University

Shanghai Jiao Tong University

Hunan Cancer Hospital

Nanjing Medical University

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Preparation of human scFv antibody against nasopharyngeal carcinoma and identification of its specificity

Acta Oto-Laryngologica (2012)

Yandong Li Yuehui Li Yan Zhao Jiajia Wang Pingli Xie

Conclusion: The selected scFv antibody could specifically recognize and target nasopharyngeal carcinoma (NPC), and could be applied to clinical diagnosis and therapy. Objective: The aim was to construct and screen fully human anti-NPC single chain Fv fusion phage libraries, and to identify the specificity of the scFv antibody. Methods: Peripheral blood mononuclear cells of patients with NPC were immunized in vitro by NPC cells and transformed by Epstein-Barr virus. The total RNAwas used to construct the scFv libraries. By means of ELISA and immunochemistry, the positively bound scFv was selected and identified. The positive scFv was fused to EGFP, and was then expressed in E. coli strain BL21 (DE3) and purified. Furthermore, we observed the binding bioactivity. Results: The fusion protein has the biological activity of binding the NPC cells and emitting green fluorescence. In targeting experiments in vivo, the results showed that the fusion protein can successfully target the NPC.

Identification

10.3109/00016489.2012.655861

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TLR3 activation inhibits nasopharyngeal carcinoma metastasis via down-regulation of chemokine receptor CXCR4

Cancer Biology & Therapy (2009)

Yu Zhang Ruili Sun Bei Liu Minjie Deng Weijia Zhang

AbstractToll-like receptor 3 (TLR3), a receptor for viral double strain RNA (dsRNA), mediates anti-viral immune response by activating the innate immunity and cross-priming CD8+ T cells. TLR3 agonists can directly trigger apoptosis in human cancer cells, and have been used as adjuvants to treat cancer patients with the aim of inducing an IFN-dependent immune response. In this study, we examined the effect of TLR3 activation on the metastasis of nasopharyngeal carcinoma (NPC). We found that NPC cells expressed TLR3 gene transcript and protein. TLR3 activation down-regulated the expression of chemokine receptor CXCR4 in a dose-dependent manner, and inhibited cell migration in response to CXCR4 ligand stromal cell-derived factor-1α (SDF-1α) in chemotaxis assays. Furthermore, TLR3 activation significantly reduced the capacity of NPC cells to form metastasis in draining lymph nodes when injected in athymic mice. The anti-metastasis activity of endogenous human TLR3 expression in cancer cells reveals a novel aspect of the multiple-faced TLR biology, which may open new clinical prospects for using TLR3 agonists to control cancer metastasis in selected cancers.

TLR3

10.4161/cbt.8.19.9437

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Citations (44)

Spaceflight alters the gene expression profile of cervical cancer cells

Chinese Journal of Cancer (2011)

Zhijie Zhang Yongqing Tong Jiajia Wang Yang Cheng Guohua Zhou

Spaceflight

Suppression subtractive hybridization

10.5732/cjc.011.10174

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Citations (11)

[MicroRNA-33a regulates the invasion of cervical cancer cells via targeting Twist1].

Jia Hu Yan-Hong Gui Pingli Xie Guancheng Li

To examine the expression of Twist1 in cervical cancer and to explore its biological function in the progression of cervical cancer. The expressions of Twist1 in 32 cervical cancers and matched normal tissues were examined by immunohistochemistry (IHC). Cell invasive ability and the expression of invasion-related genes were determined in RNAi-based Twist1-silencing HeLa cells. The relationship between Twist1 and microRNA-33a (miR-33a) in cervical cancer was studied by Pearson correlation analysis, and the roles of miR-33a in regulation of Twist1 and cell invasiveness were studied. The positive expression rate of Twist1 was 75.0% (24/32) and 21.9% (7/32) in the cervical cancer and the matched normal tissues, respectively, with significant difference between them (P<0.05). Twist1 shRNA significantly decreased the invasiveness of HeLa cells (P<0.05). Compared with the matched normal tissues, the expression of miR-33a was increased in the cervical cancer tissues, which was negatively correlated with Twist1 (r=-0.661, P<0.05). Overexpression of miR-33a could significantly suppress Twist1 expression as well as cell invasiveness (P<0.05). Twist1 is critical for the invasiveness of cervical cancer cells; miR-33a, as a tumor suppressor gene, functions as an upstream regulator of Twist1 and is involved in the invasiveness of cervical cancer cell.目的：研究转录因子Twist1在宫颈癌组织中的表达及其在宫颈癌细胞进展过程中的作用。方法：免疫组织化学检测32对临床宫颈癌组织及正常宫颈组织中Twist1的表达情况，并运用RNA干扰技术沉默宫颈癌HeLa细胞内Twist1 后检测细胞的侵袭能力以及侵袭相关基因表达的变化；采用Pearson相关性检验分析Twist1与microRNA-33a(miR-33a)在宫颈癌组织内的关系，并分析miR-33a对Twist1的调节关系及其对细胞侵袭能力的影响。结果：Twist1在宫颈癌组织和正常宫颈组织中表达的阳性率分别为75.0%(24/32)和21.9%(7/32)，二者表达差异具有统计学意义(P<0.05)；Twist1 shRNA能显著减低HeLa细胞的体外侵袭能力。宫颈癌组织中miR-33a的表达较正常组织减低(P<0.05)，并与Twist1呈负相关(r=−0.661，P<0.05)；而宫颈癌细胞内过表达miR-33a可减低Tiwst1的表达(P<0.05)，并减弱细胞的体外侵袭能力。结论：Twist1在宫颈癌的侵袭转移过程中发挥着重要的作用，miR-33a为上游调控Twist1的表达及细胞侵袭能力的抑癌基因。.

HeLa

Twist transcription factor

10.11817/j.issn.1672-7347.2015.10.002

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Genetic polymorphisms of new 22 autosomal STR loci in the Mongolian ethnic group

International Journal of Legal Medicine (2019)

Dan Wen Zedeng Yang Shule Sun Aliye Kureshi Moutanou Modeste Judes Zeye

10.1007/s00414-019-02111-3

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The Expression of Functional Chemokine Receptor CXCR4 Is Associated with the Metastatic Potential of Human Nasopharyngeal Carcinoma

Clinical Cancer Research (2005)

Jinyue Hu Xiyun Deng Xiu‐Wu Bian Guan‐Cheng Li Yongqing Tong

Abstract Purpose: Chemokine receptors are implicated in metastasis of several malignant tumors. This study was done to evaluate the contribution of chemokine receptors CXCR4 and CCR7 to metastasis of human nasopharyngeal carcinoma. Experimental Design: Reverse transcription-PCR, immunohistochemistry, and flow cytometry were used to evaluate mRNA and protein expression of CXCR4 and CCR7 in nasopharyngeal carcinoma tumor tissues and cell lines. Chemotaxis assays were used to evaluate the function of CXCR4 in nasopharyngeal carcinoma cells. Antisense CXCR4 was used to inhibit receptor expression and to block metastasis of human nasopharyngeal carcinoma cells in vivo in athymic mice. Results: CXCR4 protein was detected in tumor cells in 31 of 40 primary human nasopharyngeal carcinoma and in 13 of 15 lymph node metastases. CXCR4 transcripts were detected in eight CXCR4 protein–positive primary nasopharyngeal carcinoma tissues and seven nasopharyngeal carcinoma cell lines tested. On the other hand, the transcripts for CCR7 were detected only in four primary nasopharyngeal carcinoma tissues and in none of the nasopharyngeal carcinoma cell lines. In functional experiments, metastatic nasopharyngeal carcinoma cell lines that expressed high levels of CXCR4 were found to migrate in response to the CXCR4 ligand SDF-1α. Transfection of antisense CXCR4 in metastatic nasopharyngeal carcinoma cells inhibited the expression of CXCR4 and SDF-1α-induced cell migration in vitro and reduced the capacity of the tumor cells to form metastasis in the lungs and lymph nodes when injected in athymic mice. Conclusion: The expression of functional CXCR4 but not CCR7 is correlated with the metastatic potential of human nasopharyngeal carcinoma cells. Therefore, CXCR4 may be considered as a potential target for the prevention of nasopharyngeal carcinoma metastasis.

10.1158/1078-0432.ccr-04-1798

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Citations (71)

Construction and Selection of Human Fab Antibody Phage Display Library of Liver Cancer

Hybridoma (2009)

Xuan Shui Jian Huang Yuehui Li Pingli Xie Guancheng Li

The aim of this study was to construct the fully humanized anti-hepatoma Fab fragment phage libraries and select antibodies against hepatoma specifically. PBMCs of liver cancer patients were immunized in vitro with HpeG2 cells and were then transformed by Epstein-Barr virus (EBV). After total RNA was extracted, the heavy chain Fd and κ/λ light chain were amplified by RT-PCR and cloned into the vector pComb3 to construct the libraries of Fab fragments. The libraries were then panned by HpeG2 cells. By means of ELISA and immunochemistry, the Fab phage antibodies binding with hepatoma were selected and identified. The Fd and light chain PCR products were subsequently inserted into pComb3, and the volume of Fab libraries reached 1.7 × 107. The libraries were enriched about 138-fold by three cycles of panning. 540 phage clones were picked randomly. Using cell ELISA and immunohistochemistry with cultured cells, one clone Fab phage antibody, which had binding activity with hepatoma, was picked out. Fully humanized anti-hepatoma Fab antibody phage display libraries were constructed. One phage clone was selected and confirmed to specifically bind to hepatoma cells. The selected Fab antibody may be further developed and applied to clinical diagnosis and therapy.

10.1089/hyb.2009.0028

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Citations (11)

Clavien-Dindo classification and risk factors of gastrectomy-related complications: an analysis of 1049 patients.

Hua Xiao Pingli Xie Kunyan Zhou Xiaoxin Qiu Hong Yuan

The objective of the present study was to explore the major risk factors of surgical complications using the Clavien-Dindo classification.The case-control design was used. A total of 1049 patients who underwent radical gastrectomy in Hunan Cancer Hospital between October 2010 and August 2014 were retrospectively analyzed, including 122 patients (11.6%) with complications and 927 patients (88.4%) with no complications. Risk factors were evaluated.Following radical gastrectomy, 122 patients (11.6%) experienced a total of 151 complications. The incidence of Stages II, IIIa, IIIb, IVa, IVb and V complications was 9.6% (n = 101), 2.5% (n = 26), 1.0% (n = 11), 0.8% (n = 8), 0% (n = 0), and 0.5% (n = 5), respectively. The incidence of severe complications (Stage ≥ IIIa) was 4.8% (n = 50). Multivariate analysis showed that combined resection (Odds Ratio [OR] = 3.36, 95% confidence interval [CI]: 1.71~6.60, P < 0.01), perioperative blood transfusion (OR = 2.13, 95% CI: 1.38-3.29, P < 0.01), and BMI ≥ 25 kg/m(2) (OR = 1.98, 95% CI: 1.16-3.40, P = 0.01) were independent risk factors of complications.Combined resection, perioperative blood transfusion, and BMI ≥ 25 kg/m(2) are positively correlated with complications.

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Citations (20)

Effects of space flight exposure on cell growth, tumorigenicity and gene expression in cancer cells

Advances in Space Research (2008)

Yang Cheng Yuehui Li Zhijie Zhang Chen Luo Yongqing Tong

Spaceflight

10.1016/j.asr.2008.06.017

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Citations (3)

Omentin inhibits osteoblastic differentiation of calcifying vascular smooth muscle cells through the PI3K/Akt pathway

Amino Acids (2010)

Xin-Yun Duan Pingli Xie Yulin Ma Siyuan Tang

matrix Gla protein

10.1007/s00726-010-0800-3

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Citations (67)