Masashi Fukumoto

Kanazawa University

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Hiroto Yoshioka

Osaka University of Pharmaceutical Sciences

Tomoaki Sakamoto

Kyoto Sangyo University

Shinnosuke Kusaba

Institute of Fruit Tree and Tea Science

Yuriko Kano‐Murakami

St. Marianna University School of Medicine

Makoto Matsuoka

Fukushima University

Isomaro Yamaguchi

The University of Tokyo

Koji Aoba

Iwate University

Michael A. Venis

East Malling Research (United Kingdom)

Chiaki Ogino

Kobe University

Nobuaki Shimizu

Kanazawa University

Cooperative Institutions

The University of Tokyo

Institute of Fruit Tree and Tea Science

Ministry of Agriculture, Forestry and Fisheries

Shinshu University

Ōtani University

Kyoto University

RIKEN

Kobe University

Osaka University

Ministry of Agriculture Forestry and Fisheries

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ATP-Dependent Ca²⁺ Transport in Tonoplast Vesicles from Apple Fruit

Plant and Cell Physiology (1986)

Masashi Fukumoto Michael A. Venis

Protoplasts and vacuoles were isolated from immature apple fruit (Malus pumila Mill. cv. Golden Delicious). ATP-stimulated Ca2+ uptake was identified in both protoplast vesicles and tonoplast vesicles. The apparent Km for Ca2+ of the tonoplast transport system was 43.4 μM. The pH optima were 7.2 and 6.7 for Ca2+ transport by protoplast and tonoplast vesicles, respectively. Ca2+ transport in tonoplast vesicles was strongly inhibited by the calmodulin antagonists fluphenazine and N-(6-aminohexyl)-5-chloro-l-naphthalensulfonamide hydrochloride (W-7), while N-aminohexyl)-l-naphthalensulfonamide hydrochloride (W-5) was relatively ineffective. Addition of exogenous calmodulin stimulated transport by 35%. Ca2+ uptake was inhibited by vanadate, but not by the ionophores carbonylcyanide m-chlorophenyl hydrazone (CCCP) or valinomycin. The results indicate that apple tonoplasts have a Ca2+ transport system that is driven by the direct hydrolysis of ATP, and may be calmodulin dependent.

Protoplast

Vanadate

Valinomycin

Proton Transport

10.1093/oxfordjournals.pcp.a077125

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Citations (19)

Continuous production of phospholipase D using immobilized recombinant Streptomyces lividans

Enzyme and Microbial Technology (2006)

Chiaki Ogino Masayuki Kanemasu Masashi Fukumoto Takafumi Kubo Takamichi Yoshino

Streptomycetaceae

10.1016/j.enzmictec.2006.12.011

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Citations (6)

Changes in Cell Wall Polysaccharides in Kiwifruit (Actinidia chinensis Planch. cv. Yellow Koshin) During Softening on the Vine.

Journal of the Japanese Society for Horticultural Science (1998)

Toshiaki Makabe Hiroto Yoshioka Akira Miki Masashi Fukumoto

キウイフルーツ'イエロー紅心(仮称)'果実が樹上で軟化するメカニズムを明らかにするため, 果実硬度の低下とエチレン生成, 細胞壁構成糖の変化, 細胞壁分解酵素活性の変化との関連を検討した.1. 樹上での果実の軟化はエチレン生成の増大を伴っていなかった.2. 樹上での果実硬度の低下は, WIFの細胞壁画分のガラクツロン酸含有量の低下と一致し, 同画分の中性糖含有量の変化とは対応関係が認められなかった.3. エチレン処理により, WIFのラムノース, アラビノース, ガラクトースの消失が促進され, ガラクツロン酸はエチレン処理の有無にかかわらず消失が進んだ.以上のことから, 樹上果実の軟化はエチレンに感受しないで進行するものと思われ, ホモガラクツロナンの分解が関与している可能性が示唆された.また, エチレンに感受して進行する果実の軟化は, ホモガラクツロナンの分解とラムノガラクツロナンの分解の両方が関与している可能性が示唆された.

Actinidia chinensis

Vine

Actinidia

Actinidia deliciosa

10.2503/jjshs.67.59

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Citations (1)

Two Different Types of Homeobox Genes Exist in Apples.

Journal of the Japanese Society for Horticultural Science (1998)

Tomoaki Sakamoto Shinnosuke Kusaba Yuriko Murakami Masashi Fukumoto Shuichi Iwahori

Two different types of partial sequence of homeobox genes, named APHB1 and APHB2, were isolated from apple. From the alignment of amino acid sequences of KNOTTED1-like homeobox genes and apple PCR fragments, APHB1 was considered to be a member of class I type genes because of their similarities. APHB1 expressed itself only in shoot apical tissues, stems, and flowers, but not in mature leaves and developing fruits which is, again, very similar to class I type genes. Likewise, APHB2 is considered to be a member of class II type genes because of their homologies. APHB2 is expressed in all organs involving mature leaves and developing fruits, which is characteristic of class II type homeobox genes. These findings reveal that at least two different types of homeobox genes exist in apple. They probably bind to different genes and play different roles in controlling the developmental switches in apple.

Homeobox protein Nkx-2.5

Homeobox A1

HNF1B

EMX2

10.2503/jjshs.67.372

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Citations (2)

Effects of Calmodulin Antagonists on the Mitochondrial and Microsomal Ca<sup>2$</sup> Uptake in Apple Fruit<xref ref-type="fn" rid="fn1"><sup>1</sup></xref>

Plant and Cell Physiology (1982)

Masashi Fukumoto Koushiro Nagai

In apple fruit, active ATP-dependent microsomal Ca2$ uptake and respiration-dependent mitochondrial Ca2$ uptake were observed. The mitochondrial Ca2$ uptake was depressed by the calmodulin antagonists chlorpromazine hydrochloride (CPZ) and N-(6-aminohexyl)-5-chloro-1-naphthalene-sulfonamide hydrochloride (W-7). The Ca2$-ATPase from apple mitochondria was also inhibited by CPZ or W-7. The apparent Km value for Ca2$ in mitochondrial Ca2$ uptake (Km=0.35 mM) was similar to that of mitochondrial Ca2$-ATPase (Km=0.32 mM). The inhibitory effect of W-7 on the activity of the mitochondrial Ca2$ uptake was closely correlated with the inhibition by W-7 of mitochondrial Ca2$-ATPase (r=0.996). These findings indicate that the mitochondrial uptake of Ca2$ in apple fruit depends on the calmodulin-mediated activation of Ca2$-ATPase. The microsomal Ca2$ uptake was depressed by CPZ, suggesting that the microsomal Ca2$ uptake may also be modulated by calmodulin.

10.1093/oxfordjournals.pcp.a076492

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Citations (14)

Phylogeography of silver‐studded blue, Plebejus subsolanus (Lepidoptera: Lycaenidae), in the central mountainous regions of Japan

Entomological Science (2020)

Shouhei Ueda Takatoshi Nakatani Masashi Fukumoto Kiyoshi Maruyama Tateo Itoh

Abstract The silver‐studded blue, Plebejus subsolanus , is widely distributed in the Russian Altai mountains, northeastern China, the Korean Peninsula, and the Japanese archipelago. In Japan, the species is distributed across wide elevation ranges from the lowlands of Hokkaido to the subalpine zone of Honshu. Current subspecies classification in Japan is as follows: ssp. iburiensis , occurring in lowland grasslands in Hokkaido; ssp. yaginus in lower mountain grasslands in Honshu; and ssp. yarigadakeanus in higher mountain grasslands in Honshu. The habitat of this species has been markedly reduced due to recent habitat destruction and land‐use changes. Here, we undertook phylogeographic analyses of two subspecies, ssp. yaginus and yarigadakeanus in the central mountainous regions of Japan, based on two mitochondrial gene sequences, in order to collect information for establishing effective conservation strategies. From 57 samples from the four mountain ranges, we obtained a haplotype network comprised of 12 haplotypes. Because of the haplotype network topology, the geographic distribution of haplotypes and the correspondence of haplotype divergence to subspecies taxonomy, we provisionally divided the haplotypes into three haplogroups: YR1 and YR2, which comprised ssp. yarigadakeanus , and YG, which comprised ssp. yaginus . Mitochondrial DNA genetic differentiation generally agreed with morphological subspecies classification . The haplotype network suggested that ssp. yarigadakeanus populations had multiple origins, and the subspecies character of “bright blue of the male's wings” was assumed to have evolved independently in each subalpine meadow. We found that P. subsolanus was genetically differentiated depending upon the elevation at each mountain region, suggesting that each haplogroup should be a conservation unit.

Subspecies

Archipelago

Haplogroup

Refugium (fishkeeping)

10.1111/ens.12416

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Citations (3)

A Calmodulin and Calcium-Related Physiological Disorder (Bitter Pit) of Apples

Elsevier eBooks (1985)

Masashi Fukumoto

10.1016/b978-0-12-347230-4.50033-0

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Citations (1)

Effects of Long-Term Nitrogen Application on Tree Growth, Yield, and Fruit Qualities in a 'Jonathan' Apple Orchard.

Journal of the Japanese Society for Horticultural Science (2000)

Kenzo Komamura Akira Suzuki Masashi Fukumoto Kiminori Kato Y. Sato

マルバカイドウ台リンゴ'紅玉'樹の生長および果実生産に対する窒素施用の影響を, 草生ほ場に-N(無施用), 1N(5kg・10a-1), 2N(10kg・10a-1), 4N(20kg・10a-1)の4処理区を設け, 10∿32年生の23年間にわたり解析した.窒素施用の影響は, 幹周やせん定枝量では5年目以降で差がみられ, 4N区が明らかに優った.葉中窒素含有率は窒素施用量が多いほど高く, 処理開始3年目から差がみられ, 試験後半には全処理区間で有意差がみられた.果実生産に対する窒素施用の影響は, 品質で大きく, 収量にはほとんどの年次で処理間差がなかった.一方, 窒素施用量が多いほど着色不良な果実が多く, また着色不良果は着色良果と比較し糖度および果肉硬度が低かった.以上の結果, 窒素の多量施用は樹体生長を旺盛にするものの果実品質では負の影響が大きく, 当リンゴ園土壌では10a当たり10kg以下が適正窒素施用量であるとみなされた.また, 土壌の肥沃度によっては窒素肥料無施用でもリンゴ樹の生育と果実生産が長期にわたり維持できることが明らかとなった.

Orchard

Apple tree

10.2503/jjshs.69.617

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Citations (13)

Mechanism of the Development of a Calcium-Related Disorder (Bitter Pit) in Apple

Japan Agricultural Research Quarterly JARQ (1987)

Masashi Fukumoto Koushiro Nagai Hiroto Yoshioka Koji Aoba

Source

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Citations (5)

CHANGES OF HORMONAL CONTENTS INTRANCEGENIC TOBACCO INTRODUCED WITH OSH1 GENE