E. Machová

Slovak Academy of Sciences

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Grigorij Kogan

Directorate-General for Research and Innovation

JŠ

J. Šandula

Institute of Chemistry of the Slovak Academy of Sciences

Melánia Babincová

Comenius University Bratislava

Vladimı́r Dolez̆al

Czech Academy of Sciences

Ján Jakubík

Czech Academy of Sciences, Institute of Physiology

Peter Capek

Institute of Chemistry of the Slovak Academy of Sciences

Darina Chorvatovičová

Slovak Academy of Sciences

ТА

Т. А. Короленко

Scientific Research Institute of Biochemistry

Anna Ebringerová

Institute of Chemistry of the Slovak Academy of Sciences

Slavomı́r Bystrický

Institute of Chemistry of the Slovak Academy of Sciences

Cooperative Institutions

Slovak Academy of Sciences

Institute of Chemistry of the Slovak Academy of Sciences

Comenius University Bratislava

Czech Academy of Sciences, Institute of Physiology

Russian Academy of Sciences

Slovak Medical University

Czech Academy of Sciences

Research Institute of Physiology and Fundamental Medicine of the Siberian Branch of the Russian Academy of Medical Sciences

Institute of Physiology and Basic Medicine

Institute of Experimental Pharmacology and Toxicology of the Slovak Academy of Sciences

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Chemical modification of beechwood xylan withp-carboxybenzyl bromide

Journal of Applied Polymer Science (1996)

Anna Ebringerová Z. Novotná Marta Kačuráková E. Machová

Xylan isolated from beechwood was alkylated with p-carboxybenzyl bromide in aqueous alkali. The alkylation by varying stochiometry and reaction temperature yielded carboxybenzylated xylan derivatives with a degree of substitution which ranged from 0.05 to 0.25. The reaction products were characterized by elementary analysis and FTIR and UV spectroscopy. As tested by HPGPC, no degradation of the xylan chains resulted up to a reaction temperature of 40°C. The derivatives show moderate surface activity. © 1996 John Wiley & Sons, Inc.

10.1002/(sici)1097-4628(19961114)62:7<1043::aid-app10>3.0.co;2-7

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Citations (16)

Immunological properties of complex conjugates based on Vibrio cholerae O1 Ogawa lipopolysaccharide antigen

Clinical & Experimental Immunology (2006)

Ema Paulovičová E. Machová A Hos̆tacká Slavomı́r Bystrický

Summary Host protection by humoral immunity against Vibrio cholerae O1 confers lipopolysaccharide (LPS)-specific vibriocidal antibodies. Levels of relevant specific antibodies are closely related to complement-mediated inactivation of the vibrios inoculum, especially on the mucosal surface of intestine. We have tested complex V. cholerae O1 Ogawa-detoxified lipopolysaccharide (dLPS) conjugates. The first conjugate contained glucan both as the immunomodulator and the matrix; the second conjugate contained immunologically inert amylose as matrix. Both d-LPS conjugates contain multiply attached dLPS antigen. These conjugates elicited a statistically significant increase of antigen-specific IgG levels in mice (P < 0·001 and P < 0·05, respectively). The specific anti-conjugate IgG and IgA response after the second (booster) dose were significantly higher compared to pre-immune and whole-cell response. The most effective vibriocidal activity was observed in the case of conjugate, with glucan as the matrix. The highest correlation was found between vibriocidal activity and specific IgG2b (r = 0·765) and IgA (r = 0·887) sera levels. The determination of specific IgG subclasses and IgG2a + 2b/IgG1 ratio revealed a dominant TH1 cell response crucial for effective vaccine candidate.

Conjugate

Humoral immunity

Immunoglobulin A

10.1111/j.1365-2249.2006.03077.x

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Citations (12)

Effect of gluco-monosaccharides and different conditions on digestion of hyaluronan by testicular hyaluronidase.

PubMed (2002)

Peter Bystrický E. Machová N. Kolarova

The changes of molecular size of hyaluronan during enzymatic reaction of bovine testicular hyaluronidase at different conditions are monitored by size exclusion high performance liquid chromatography. The effect of glucuronate, galacturonate, glucosamines and pyridoxin as potential inhibitors of hydrolysis is evaluated. The most effective of all tested inhibitors was the presence of glucuronate which not only inhibited the hydrolysis, but also initiated enzymatic reconstruction by transglycosylation reaction at pH 7.0 and absence of any buffer or salt. That effect was not found in the presence of a salt or with any other of the compounds tested.

Glucuronates

Hyaluronidase

Monosaccharide

Enzymatic Hydrolysis

Digestion

Phosphate buffered saline

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Ultrasonic depolymerization of the chitin–glucan complex from Aspergillus niger and antimutagenic activity of its product

Ultrasonics Sonochemistry (1999)

E. Machová Grigorij Kogan Darina Chorvatovičová J. Šandula

Aspergillus niger

Depolymerization

Sonication

10.1016/s1350-4177(98)00024-8

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Citations (21)

Scavenging and antioxidant activities of immunomodulating polysaccharides isolated from Salvia officinalis L.

International Journal of Biological Macromolecules (2008)

Peter Capek E. Machová J. Turjan

Salvia officinalis

Salvia

Ammonium oxalate

10.1016/j.ijbiomac.2008.10.007

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Citations (60)

Glucans as antimutagens

D. Chorvatovichova J. Šandula E. Machová Peter Eckl Nikolaus Bresgen

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Antioxidant activity of medicinal plant polysaccharides

Fitoterapia (2006)

Alžbeta Kardošová E. Machová

10.1016/j.fitote.2006.05.001

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Citations (174)

Enzymatic and ultrasonic depolymerization of carboxymethylated β‐1,3‐D‐glucans derived from saccharomyces cerevisiae

Journal of Applied Polymer Science (1995)

E. Machová Grigorij Kogan Juraj Alföldi L. Šotés J. Šandula

Abstract Enzymatic and ultrasonic degradations of the carboxymethyl derivative of the β‐1,3‐D‐glucan, isolated from the cell walls of baker's yeast Saccharomyces cerevisiae , are described. The samples obtained upon partial depolymerization of the two carboxymethylated glucans with the substitution degree of 0.56 and 0.91 were characterized by the distributions of their molecular weights and 13 C‐NMR spectra. © 1995 John Wiley & Sons, Inc.

Depolymerization

10.1002/app.1995.070550506

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Citations (49)

Nicotine indirectly increases acetylcholine release in rat striatum

Journal of Neurochemistry (2003)

E. Machová V. Žemličková Vladimı́r Doležal

We investigated the influence of nicotine on the release of ACh from rat striatal slices in the presence of domperidone to remove dopamine inhibition of ACh release. In medium facilitating glutamatergic transmission (with glycine and without Mg) nicotine increased the release of ACh evoked by mild potassium depolarization. This effect of nicotine was not present in resting conditions or when the tissue was fully depolarized. Enhancement of the release of ACh was prevented by tetrodotoxine, nicotinic antagonist mecamylamine, and glutamate NMDA antagonist MK 801. Our results suggest that nicotine increases ACh release indirectly by stimulation of nicotinic receptors on glutamatergic nerve terminals leading to the enhancement of glutamate release. Released glutamate in turn increases firing of cholinergic interneurons and ACh release. Nicotinic receptors on striatal glutamatergic nerve terminals perhaps play a role in the mechanism of striatal cholinergic hyperactivity observed in Parkinson's disease. Acknowledgements: Supported by grants GACR 305/01/0283 and GAAV 5011206.

Mecamylamine

Nicotinic Antagonist

10.1046/j.1471-4159.85.s2.15_3.x

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Citations (1)

[Inflammation of genitalia following artificial pregnancy interruption].

PubMed (1970)

J Divis Z Novotný E. Machová Sten Jansa

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