James M. Crosslin

U.S. Vegetable Laboratory

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Joseph E. Munyaneza

United States Department of Agriculture

Jeremy L. Buchman

San Joaquin Valley Agricultural Sciences Center

Venkatesan G. Sengoda

United States Department of Agriculture

Kylie D. Swisher

Agricultural Research Service

Philip B. Hamm

Oregon State University

Launa L. Hamlin

United States Department of Agriculture

Rosemarie W. Hammond

Beltsville Agricultural Research Center

Alexander V. Karasev

University of Idaho

J. E. Upton

Iowa State University

Ing‐Ming Lee

Beltsville Agricultural Research Center

Cooperative Institutions

United States Department of Agriculture

Agricultural Research Service

Oregon State University

Washington State University

University of Idaho

Cornell University

University of Florida

San Joaquin Valley Agricultural Sciences Center

University of Georgia

Swedish University of Agricultural Sciences

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Low-Level Detection ofCandidatusLiberibacter Solanacearum inBactericera cockerelli(Hemiptera: Triozidae) by 16s rRna Pyrosequencing: Table 1.

Environmental Entomology (2013)

Alex P. Arp Rebekah I. Chapman James M. Crosslin Blake Bextine

Journal Article Low-Level Detection of Candidatus Liberibacter Solanacearum in Bactericera cockerelli (Hemiptera: Triozidae) by 16s rRna Pyrosequencing Get access Alex P. Arp, Alex P. Arp 2 1Department of Biology, University of Texas at Tyler, 3900 University Blvd., Tyler, TX 75799. 2Corresponding author, e-mail: aalex@patriots.uttyler.edu. Search for other works by this author on: Oxford Academic PubMed Google Scholar Rebekah Chapman, Rebekah Chapman 1Department of Biology, University of Texas at Tyler, 3900 University Blvd., Tyler, TX 75799. Search for other works by this author on: Oxford Academic PubMed Google Scholar James M. Crosslin, James M. Crosslin 3Vegetable and Forage Crops Research Unit, USDA-ARS, Prosser, WA 99350. Search for other works by this author on: Oxford Academic PubMed Google Scholar Blake Bextine Blake Bextine 1Department of Biology, University of Texas at Tyler, 3900 University Blvd., Tyler, TX 75799. Search for other works by this author on: Oxford Academic PubMed Google Scholar Environmental Entomology, Volume 42, Issue 5, 1 October 2013, Pages 868–873, https://doi.org/10.1603/EN12260 Published: 01 October 2013 Article history Received: 14 September 2012 Accepted: 19 July 2013 Published: 01 October 2013

Pyrosequencing

10.1603/en12260

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Citations (10)

RNA-Seq analysis of resistant and susceptible potato varieties during the early stages of potato virus Y infection

BMC Genomics (2015)

Aymeric Goyer Launa L. Hamlin James M. Crosslin Alex Buchanan Jeff H. Chang

Potato virus Y (PVY) is one of the most important plant viruses affecting potato production. The interactions between potato and PVY are complex and the outcome of the interactions depends on the potato genotype, the PVY strain, and the environmental conditions. A potato cultivar can induce resistance to a specific PVY strain, yet be susceptible to another. How a single potato cultivar responds to PVY in both compatible and incompatible interactions is not clear.In this study, we used RNA-sequencing (RNA-Seq) to investigate and compare the transcriptional changes in leaves of potato upon inoculation with PVY. We used two potato varieties: Premier Russet, which is resistant to the PVY strain O (PVY(O)) but susceptible to the strain NTN (PVY(NTN)), and Russet Burbank, which is susceptible to all PVY strains that have been tested. Leaves were inoculated with PVY(O) or PVY(NTN), and samples were collected 4 and 10 h post inoculation (hpi). A larger number of differentially expressed (DE) genes were found in the compatible reactions compared to the incompatible reaction. For all treatments, the majority of DE genes were down-regulated at 4 hpi and up-regulated at 10 hpi. Gene Ontology enrichment analysis showed enrichment of the biological process GO term "Photosynthesis, light harvesting" specifically in PVY(O)-inoculated Premier Russet leaves, while the GO term "nucleosome assembly" was largely overrepresented in PVY(NTN)-inoculated Premier Russet leaves and PVY(O)-inoculated Russet Burbank leaves but not in PVY(O)-inoculated Premier Russet leaves. Fewer genes were DE over 4-fold in the incompatible reaction compared to the compatible reactions. Amongst these, five genes were DE only in PVY(O)-inoculated Premier Russet leaves, and all five were down-regulated. These genes are predicted to encode for a putative ABC transporter, a MYC2 transcription factor, a VQ-motif containing protein, a non-specific lipid-transfer protein, and a xyloglucan endotransglucosylase-hydroxylase.Our results show that the incompatible and compatible reactions in Premier Russet shared more similarities, in particular during the initial response, than the compatible reactions in the two different hosts. Our results identify potential key processes and genes that determine the fate of the reaction, compatible or incompatible, between PVY and its host.

Potato virus Y

Strain (injury)

10.1186/s12864-015-1666-2

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Citations (79)

A Global Comparison ofBactericera cockerelli(Hemiptera: Triozidae) Microbial Communities

Environmental Entomology (2014)

Alex P. Arp Joseph E. Munyaneza James M. Crosslin John T. Trumble Blake Bextine

The potato psyllid (Bactericera cockerelli Sulc) is an economically important insect pest of solanaceous crops such as potato, tomato, pepper, and tobacco. Historically, the potato psyllid's range included central United States, Mexico, and California; more recently, populations of this insect have been reported in Central America, the Pacific Northwest, and New Zealand. Like most phytophagous insects, potato psyllids require symbiotic bacteria to compensate for nutritional deficiencies in their diet. Potato psyllids harbor the primary symbiont, Candidatus Carsonella ruddii, and may also harbor many secondary symbionts such as Wolbachia sp., Sodalis sp., Pseudomonas sp., and others. These secondary symbionts can have an effect on reproduction, nutrition, immune response, and resistances to heat or pesticides. To identify regional differences in potato psyllid bacterial symbionts, 454 pyrosequencing was performed using generic 16S rRNA gene primers. Analysis was performed using the Qiime 1.6.0 software suite, ARB Silva, and R. Operational taxonomic units were then grouped at 97% identity. Representative sequences were classified to genus using the ARB SILVA database. Potato psyllids collected in California contained a less diverse microbial community than those collected in the central United States and Central America. The crop variety, collection year, and haplotype did not seem to affect the microbial community in potato psyllids. The primary difference between psyllids in different regions was the presence and overall bacterial community composition of Candidatus Carsonella ruddii and Wolbachia.

Symbiotic bacteria

10.1603/en13256

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Citations (30)

Haplotypes of the Potato Psyllid, Bactericera cockerelli, on the Wild Host Plant, Solanum dulcamara, in the Pacific Northwestern United States

American Journal of Potato Research (2013)

Kylie D. Swisher Venkatesan G. Sengoda Jacob Dixon Erik R. Echegaray Alexzandra F. Murphy

Overwintering

Solanum tuberosum

10.1007/s12230-013-9330-3

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Citations (45)

Genetic Diversity of the Ordinary Strain of Potato virus Y (PVY) and Origin of Recombinant PVY Strains

Phytopathology (2011)

Alexander V. Karasev Xiaojun Hu Celeste J. Brown Camille Kerlan O. V. Nikolaeva

The ordinary strain of Potato virus Y (PVY), PVY O , causes mild mosaic in tobacco and induces necrosis and severe stunting in potato cultivars carrying the Ny gene. A novel substrain of PVY O was recently reported, PVY O -O5, which is spreading in the United States and is distinguished from other PVY O isolates serologically (i.e., reacting to the otherwise PVY N -specific monoclonal antibody 1F5). To characterize this new PVY O -O5 subgroup and address possible reasons for its continued spread, we conducted a molecular study of PVY O and PVY O -O5 isolates from a North American collection of PVY through whole-genome sequencing and phylogenetic analysis. In all, 44 PVY O isolates were sequenced, including 31 from the previously defined PVY O -O5 group, and subjected to whole-genome analysis. PVY O -O5 isolates formed a separate lineage within the PVY O genome cluster in the whole-genome phylogenetic tree and represented a novel evolutionary lineage of PVY from potato. On the other hand, the PVY O sequences separated into at least two distinct lineages on the whole-genome phylogenetic tree. To shed light on the origin of the three most common PVY recombinants, a more detailed phylogenetic analysis of a sequence fragment, nucleotides 2,406 to 5,821, that is present in all recombinant and nonrecombinant PVY O genomes was conducted. The analysis revealed that PVY N:O and PVY N-Wi recombinants acquired their PVY O segments from two separate PVY O lineages, whereas the PVY NTN recombinant acquired its PVY O segment from the same lineage as PVY N:O . These data suggest that PVY N:O and PVY N-Wi recombinants originated from two separate recombination events involving two different PVY O parental genomes, whereas the PVY NTN recombinants likely originated from the PVY N:O genome via additional recombination events.

Potato virus Y

Potyviridae

Strain (injury)

10.1094/phyto-10-10-0284

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Citations (111)

RNA-Seq analysis of early infected potato leaves by potato virus Y in resistant and susceptible potato varieties

BMC Genomics (2015)

Aymeric Goyer Launa L. Hamlin James M. Crosslin Alex Buchanan Jeff H. Chang

Potato virus Y

Potato virus X

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Impact of Different Potato Psyllid Populations on Zebra Chip Disease Incidence, Severity, and Potato Yield

Yaredai zhiwu kexue (2008)

Joseph E. Munyaneza Jeremy L. Buchman J. E. Upton John A. Goolsby James M. Crosslin

Zebra chip (ZC) is an emerging and damaging potato disease that is causing millions of dollars in losses to the potato industry in the southwestern United States, Mexico, and Central America. ZC plant symptoms resemble those caused by potato purple top and psyllid yellows diseases. Tubers produced by ZC-infected potato plants exhibit internal necrosis that affects the entire tuber. Fried chips processed from ZC-infected tubers have a characteristic striped pattern of necrosis and are unmarketable. This potato disease has recently been associated with the potato psyllid ( Bactericera cockerelli Sulc). A field experiment was conducted in southern Texas, under controlled cage conditions, to document the impact of different geographic populations of potato psyllid on ZC incidence, severity, and potato yield. Nine different colonies of psyllids were used in the study. Results showed that potato plants exposed to potato psyllids developed typical ZC symptoms in raw tubers and fried chips. ZC incidence in potato plants ranged from 0 to 100%. Not all insects used in the study were infective and only six psyllid colonies induced ZC symptoms in potatoes. No ZC symptoms were observed in psyllid-free control plots. Results indicated that ZC symptom severity in fried chips was correlated with ZC severity in raw tubers and with tuber weight. The impact of potato psyllid on potato yield and processing quality was highly significant. The number of commercially acceptable tubers per plant was significantly reduced and up to 93% potato yield loss occurred when potato plants were exposed to psyllids. Tubers from ZC-infected plants produced unmarketable potato chips.

Root crops

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A new haplotype of “Candidatus Liberibacter solanacearum” identified in the Mediterranean region

European Journal of Plant Pathology (2012)

Warrick Nelson Venkatesan G. Sengoda A. Alfaro‐Fernández I. Font James M. Crosslin

10.1007/s10658-012-0121-3

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Citations (99)

New Assays for Rapid Detection of Beet Leafhopper-Associated Plant Pathogens, ‘Candidatus Phytoplasma trifolii’, Beet Curly Top Virus, and Spiroplasma citri

Plant Disease (2023)

Kylie D. Swisher Grimm Christopher Gorman James M. Crosslin

The beet leafhopper

Spiroplasma

Phytoplasma

Q fever

Plant disease

10.1094/pdis-04-23-0769-re

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Citations (1)

Detection of Tobacco Rattle Virus RNA in Processed Potato Chips Displaying Symptoms of Corky Ringspot Disease

HortScience (2009)

James M. Crosslin

In Apr. 2008, commercially purchased processed potato chips were observed with dark brown arcs and rings typical of corky ringspot disease. This disease is caused by infection with tobacco rattle virus (TRV). A portion of RNA 1 of TRV was amplified by reverse transcription polymerase chain reaction (RT-PCR) from each of eight discolored chips from three different bags purchased at three locations. Sequence analysis of the 463-bp amplicons confirmed that the products were indeed TRV in origin and were 97% identical to TRV sequences of isolates originating in Washington, Florida, and Wisconsin. Extracts from the symptomatic RT-PCR-positive chips were not infectious when mechanically inoculated onto tobacco leaves. To the author's knowledge, this is the first report of the detection of plant virus RNA in a food product after high-temperature frying.

Tobacco rattle virus

Amplicon

10.21273/hortsci.44.6.1790

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Citations (2)