Gao Shouyi

NARI Group (China)

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Biao Kan

National Institute for Communicable Disease Control and Prevention

Yanqing Liu

Columbia University Irving Medical Center

Yongyu Rui

Southern Medical University

Guoming Qi

National Institute for Communicable Disease Control and Prevention

Lijuan Zhang

Tennessee State University

Shengjun Jiang

Jiangsu University

Qing Tang

Huazhong Agricultural University

Guangcai Duan

Zhengzhou University

Yang Hongyan

Nanjing Agricultural University

Xiaoying Cui

Centre for Mental Health

Cooperative Institutions

Chinese Center For Disease Control and Prevention

National Institute for Communicable Disease Control and Prevention

Center for Disease Control

Sun Yat-sen University

State Key Laboratory of Infectious Disease Prevention and Control

Harbin Medical University

Peking University

Zhejiang University

Chinese Academy of Sciences

Shandong University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Cloning of the complete genome set and sequence analysis of the VP4, VP6, and VP7 genes of a novel adult diarrhea rotavirus strain J19

Bingdu xuebao (2006)

Shengjun Jiang Shaozhong Ji Qing Tang Yang Hongyan Xiaoying Cui

For better understanding the molecular characteristics of the genes and proteins of a novel adult diarrhea rotavirus strain J19(NADRV-J19),the complete genome set of NADRV-J19 was amplified by an improved single primer sequence-independent amplification method,cloned into pMD18-T vector,and sequenced. Protein sequences of VP4,VP6,and VP7 of J19 strain were aligned with those of other rotaviruses and phylogenetic analysis based on VP6 sequence was made with the other rotaviruses.Sequence analysis of 11 full-length genes of J19 revealed that genomic segments 3,6,and 9 of NADRV-J19 encoding VP4,VP6,and VP7 were 2512,1287,and 820bp in length,and their deduced proteins were 823aa,396aa,and 258aa respectively.Identities of VP4,VP6,and VP7 proteins of J19 strain to that of group B rotavirus strains CAL,IDIR,and ADRV were 27.6%,38.5%,and 22.3% respectively.Phylogenetic analysis based on VP6 proteins showed that J19 was positioned closely to outgroups and the ancestral nodes of rotavirus group A,B,and C lineages,although with a preferred association with group B lineages.The above results showed that VP4,VP6,and VP7 proteins of J19 differed significantly from those of the other rotaviruses.J19 would be a representative strain of a novel rotavirus group and would be a strain closely related to the evolution and origin of group B rotaviruses.Up to now,this is the first report concerning to the cloning of 11 gene segments of NADRV-J19 and sequence analysis of the genes of NADRV-J19 encoding VP4,VP6,and VP7 proteins.

Strain (injury)

Cloning (programming)

Group A

Source

Cite

Citations (0)

Advances in molecular typing of Mycobacterium tuberculosis.

Zhongguo renshougonghuanbing zazhi (2010)

Bing Lv Gao Shouyi Wan Kang-lin

Source

Cite

Citations (0)

Study on structures and functions of new types of ig1-rstR-ig2 of Vibrio cholerae O139 serogroup

Zhongguo redai yixue (2009)

Yongyu Rui Kan Baio Gao Shouyi

Objective To investigate he structures and functions of new types of ig1-rstR-ig2 of Vibrio cholerae(VC) O139 serogroup.Methods Genes including end of core region and start of RS region of CTXφ or nct-CTXφ genome were obtained with PCR method.Operator of rstA was obtained with PCR method and cloned into the front of lacZ gene of plasmid pRS591,and then transformed into JM109 strain.β-galactosidase of recombinant JM109 was detected quantitatively for the activity of each rstA promoter.Different type of rstR gene and promoter region was cloned into plasmid pACYC184 differentially,and each recombinant plasmid pACYC184 was transformed into recombinant JM109 strain including different recombinant plasmid pRS591 differentially.β-galactosidase of each JM109 strain including two recombinant plasmids was detected quantitatively for observation of regulating function of each type of RstR on expression of each type of rstA gene.Results The homology of calc,ET and newO139 types of ig1,rstR and ig2 were 85.1%～91.6%,9.4%～17.7% and 8.0%～ 26.2%.Activities of promoters of rstA were different,those of calc and ET types were higher,that of new O139 type was lower.Repression of expression of rstA by the same type of RstR was prominent,but was not prominent in other types.Conclusions Structures and functions of calc,ET and newO139 types of ig1-rstR-ig2 of O139 serogroup Vibrio cholerae were elucidated.

Source

Cite

Citations (0)

Study on structure of new CTXΥ and nct-CTXΥ genomes coexisted in 0139 serogroup Vibrio cholerae strains.

Zhongguo redai yixue (2009)

Yongyu Rui Biao Kan Gao Shouyi

Objective To study the structure of new CTXφ and nct-CTXφ genomes coexisted in O139 serogroup Vibrio cholerae(VC) strains.Methods Genes including end of core region of CTXφ or nct-CTXφ and start of genome were gotten by using PCR method,and were digested with RsaI and BglII differentially,and tested with PCR using specific primers to indentify type of rstR-ig2.Genes including part of TLC and RS region,and genes including part of RTX and core region of CTXφ or nct-CTXφ,were gotten by using PCR method.Results The typical O139VC strains with rstR-ig2 types of CTXφ and/or nct-CTXφ were FJ9510 strain including CTXETφ,FJ98352 strain including CTXETφ and nct-CTX calcφ,FJ99364 strain including CTXETφ and nct-CTXnewO139φ and nct-CTXcalcφ,JS9803 strain including CTXETφ and nct-CTX newO139φ,and JX94484 strain including CTXETφ and nct-CTXnewETφ.46 bp of 3′ part of zot genes of CTXφ and nct-CTXφ were compared and 20 different sites were observed,and 11 of presumptive 15 AAA were different.Genes near CTXφ genomes of O139VC strains were TLC and RTX clusters,but genes near nct-CTXφ genomes were new or unidentified genes.Conclusions Changeable genes of CTXφ and nct-CTXφ of O139VC and insertion genes in chromosome were elucidated.

Strain (injury)

BglII

Source

Cite

Citations (0)

Comparisons on the molecular characteristics of Vibrio cholerae 0139 isolated from China, India and Bangladesh

Zhonghua wei Sheng wu xue he Mian yi xue za zhi = Zhonghua Weishengwuxue he Mianyixue Zazhi (1994)

Guangcai Duan Gao Shouyi Qi Guoming Yanqing Liu Cailian Liu

Cholera

Source

Cite

Citations (0)

Integration analysis of CTXphi in two chromosomes of Vibrio cholerae

Zhonghua wei Sheng wu xue he Mian yi xue za zhi = Zhonghua Weishengwuxue he Mianyixue Zazhi (2005)

Deqiao Tian Meiying Yan Gao Shouyi Biao Kan

To investigate whether CTXphi can integrate into the chromosome of the TLC negative and non-toxigenic Vibrio cholerae strains, and whether it can integrate into V. cholerae chromosomeⅡ. The corresponding chromosomal fragments of the CTX~(-) TLC~(-) strains were sequenced to detect the presence of the reported CTXΦchromosome integrate site attB. The recombinant suicide plasmid pKRSn and pKRSe which carrying RS region and attP of CTXphi were transformed into CTX~(-) TLC~(-) and CTX~(-) TLC~(+) strains, to analyze the chromosome integration and their integration sites. CTX~(-)TLC~(-) strains have a short sequence similar with attP of CTXphi. In CTX~(+)TLC~(-) strains, CTXphi integrates into chromosome II. pKRSn and PKRSe can integrate into chromosome I in TLC~(+) and TLC~(-) strain, in TLC~(-) strain they are found to integrate into chromosome II. CTXphi can integrate into chromosomes of V. cholerae through the similar integrate sites. It also suggests that TLC may play a role in the chromosome I integration of CTXphi.

Source

Cite

Citations (0)

Sequence Analysis of the VP2 and VP3 Genes of a Novel Adult Diarrhea Rotavirus Strain J19

Bingdu xuebao (2006)

Gao Shouyi

Genes of a novel adult diarrhea rotavirus(NADRV) strain J19 were amplified by an improved single primer sequence-independent amplification method,cloned into pMD18-T vector,sequenced and analyzed.Genomic segments 2,4 encoding VP2,VP3 of NADRV-J19 were 2969bp,2204bp in length,and the deduced proteins were 973aa and 719aa,respectively.Identity of VP2 of J19 to human group B rotavirus strain IDIR was 47.2 % and that of VP3 of J19 to human group C rotavirus strain Cowden was 25.1%.Phylogenetic analysis of VP2 showed that NADRV-J19 positioned closely to outgroup and the ancestral nodes of group A,B and C lineages, although with a preferred association with group B lineage,which corresponded to the phylogenetic analysis of VP6.The above results allowed us to deduce a conclusion that J19 would be a strain closely related to the evolution and origin of group B rotaviruses and they also indicated that VP2 would be valuable for studying the evolution and origin of rotaviruses.This study is the first report on sequence analysis of genes of NADRV-J19 encoding VP2 and VP3.

Lineage (genetic)

Strain (injury)

Group A

Source

Cite

Citations (0)

A new live cholera vaccine candidate constructed by gene recombination

Zhonghua wei Sheng wu xue he Mian yi xue za zhi = Zhonghua Weishengwuxue he Mianyixue Zazhi (2005)

Meiying Yan Diao BaoWei Haiyan Qiu Yanqing Liu Gao Shouyi

A chromosomal fragment containing TLC element, attRS of CTX#PHI# integration site, and RTX cluster responsible for the cytotoxic activity for mammalian cells, were deleted through gene recombination approach from the cholera vaccine prototype strain IEM101. The protective genes of rstR related to CTX#PHI# superinfection exclusion, and toxin subunit gene ctxB were integrated in the same location simultaneously to generate the candidate IEM109. Expression of ctxB was detected with GM1-ELISA, and the cytotoxic effect was evaluated in Hep-2 culture. TLC genes, attRS and rtxAC were deleted while ctxB and rstR were integrated in the same site on the IEM101 chromosome DNA. ctxB was expressed and the cytotoxic effect on Hep-2 was negative. We constructed a new candidate of our vaccine candidate series.

Cholera toxin

Source

Cite

Citations (0)

Analysis of the comprehensive assessment of the maritime college graduates in the areas under Tianjin MSA

China Maritime Safety (2008)

Gao Shouyi

This thesis designs a questionnaire on seafarer's general qualification through analyzing the capability requirements on seafarers.The questionnaire was used by the seafarer training organizations in the area under Tianjin MSA for seafarer quality assessment.This paper also provides some relevant suggestions.

Source

Cite

Citations (0)

Sequence analysis of the RNA--dependent RNA polymerase gene of a novel adult diarrhea rotavirus strain J19

Zhonghua wei Sheng wu xue he Mian yi xue za zhi = Zhonghua Weishengwuxue he Mianyixue Zazhi (2006)

Shengjun Jiang Shaozhong Ji Qing Tang Yang Hongyan Xiaoying Cui

Strain (injury)

Sequence (biology)

Source

Cite

Citations (0)