Shimiao Bu

South China University of Technology

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Yufeng Liu

South China University of Technology

Yuehong Zhang

South China University of Technology

Xiao-Jun Wu

South China University of Technology

Zheng Zhao

National Clinical Research Center for Digestive Diseases

Xiangyu Shi

Acumen (United States)

Lang Huang

Qingdao Institute of Bioenergy and Bioprocess Technology

Zongqin Xiang

South China University of Technology

Ying Yang

First Affiliated Hospital of Jinan University

Liting Zhang

South China University of Technology

Jie Zhang

Guangdong Province Women and Children Hospital

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South China University of Technology

Guangzhou Medical University

Guangzhou First People's Hospital

Guangdong Province Women and Children Hospital

Sun Yat-sen University

Chinese Academy of Sciences

First Affiliated Hospital of Jinan University

Southern Medical University

Zhejiang University

Sun Yat-sen University Cancer Center

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MDSCs promote pathological angiogenesis in ocular neovascular disease

Biomedicine & Pharmacotherapy (2024)

Xiaojun Wu Limei Zhong Jun Yu Ning Wang Shimiao Bu

Ocular neovascular diseases, which contribute significantly to vision loss, lack effective preventive treatments. Recent studies have highlighted the significant involvement of immune cells in neovascular retinopathy. Myeloid-derived suppressor cells (MDSCs) promote the development of neovascularization, but it is unknown whether they participate in pathological neovascularization and whether they are expected to be a therapeutic target.

10.1016/j.biopha.2024.117222

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Downregulation of MerTK in circulating T cells of patients with non-proliferative diabetic retinopathy

Frontiers in Endocrinology (2025)

Shimiao Bu Jiang-Yue Ling Xiao-Jun Wu Liting Zhang Xiangyu Shi

To explore the differential gene expression in peripheral blood immune cells of individuals with type 2 diabetes mellitus (DM), comparing those with and without non-proliferative diabetic retinopathy (NPDR). From a pool of 126 potential participants, 60 were selected for detailed analysis. This group included 12 healthy donors (HDs), 22 individuals with DM, and 26 with NPDR. We analyzed peripheral blood mononuclear cells (PBMCs) using RNA sequencing and quantitative PCR (qPCR) to pinpoint differentially expressed genes (DEGs). Western blot and flow cytometry were also employed to evaluate the protein expression of specific genes. In patients with NPDR compared to those with DM alone, MerTK-a gene implicated in inherited retinal dystrophies due to its mutations-was notably downregulated in PBMCs. Through flow cytometry, we assessed the protein levels and cellular distribution of MerTK, finding a predominant expression in monocytes and myeloid-derived suppressor cells (MDSCs), with a marked reduction in CD4+ and CD8+ T cells, as well as in natural killer T (NKT) cells. Patients with DM demonstrated a significant deviation in the PBMCs composition, particularly in B cells, CD4+ T cells, and NK cells, when compared to HDs. The study indicates that MerTK expression in T cells within PBMCs could act as a viable blood biomarker for NPDR risk in patients with DM. Furthermore, the regulation of T cells by MerTK might represent a critical pathway through which DM evolves into NPDR.

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10.3389/fendo.2024.1509445

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Downregulation of MerTK in Circulating T cells of Non-Proliferative Diabetic Retinopathy Patients

Research Square (Research Square) (2024)

Yong Liu Shimiao Bu Zheng Zhao Xiao-Jun Wu Liting Zhang

Abstract Objective To explore the differential gene expression in peripheral blood immune cells of individuals with type 2 diabetes mellitus (DM), comparing those with and without non-proliferative diabetic retinopathy (NPDR).Methods From 117 potential participants, 51 were selected for detailed analysis: 9 healthy donors (HDs), 19 with DM, and 23 with NPDR. We analyzed peripheral blood mononuclear cells (PBMCs) using RNA sequencing and qPCR to identify differentially expressed genes (DEGs) and used flow cytometry to assess protein expression.Results In NPDR patients compared to those with DM alone, MerTK—a gene linked to inherited retinal dystrophies—was notably downregulated in PBMCs. Flow cytometry revealed MerTK predominantly in monocytes and myeloid-derived suppressor cells (MDSCs), with reduced expression in CD4 + and CD8 + T cells and natural killer T (NKT) cells. DM patients showed significant deviations in PBMC composition, especially in B cells, CD4 + T cells, and NK cells, compared to HDs.Conclusions The study indicates that MerTK expression in T cells within PBMCs could act as a viable blood biomarker for NPDR risk in DM patients. Furthermore, the regulation of T cells by MerTK might represent a critical pathway through which DM evolves into NPDR.

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10.21203/rs.3.rs-4791734/v1

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