Guohong Huang

Dartmouth–Hitchcock Medical Center

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Wenbo Xu

Second Affiliated Hospital of Harbin Medical University

Zhen Zhu

National Institute for Viral Disease Control and Prevention

Dongmei Yan

National Institute for Viral Disease Control and Prevention

Shuangli Zhu

National Institute for Viral Disease Control and Prevention

Yong Zhang

National Institute for Viral Disease Control and Prevention

Xiaolei Li

Soochow University

Hui Cui

Hainan Normal University

Wenbo Xu

Third Affiliated Hospital of Southern Medical University

Lina Wang

Jiangxi Science and Technology Normal University

Qiang Sun

National Institute for Viral Disease Control and Prevention

Cooperative Institutions

Chinese Center For Disease Control and Prevention

Center for Disease Control

National Institute for Viral Disease Control and Prevention

Capital Medical University

Centers for Disease Control and Prevention

Chinese Academy of Medical Sciences & Peking Union Medical College

Chinese Academy of Sciences

Peking University

Sun Yat-sen University

Sichuan University

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Correction: Viral Etiology of Acute Respiratory Infection in Gansu Province, China, 2011

PLoS ONE (2013)

Guohong Huang Deshan Yu Naiying Mao Zhen Zhu Hui Zhang

Background: Acute respiratory infections (ARIs) are the leading cause of children and their leading killer.ARIs are responsible for at least six percent of the world's disability and death.Viruses are one of the most common agents causing ARIs.Few studies on the viral etiology and clinical characteristics of ARIs have been performed in the northwest region of China, including Gansu Province.Methods: Clinical and demographic information and throat swabs were collected from 279 patients from January 1st to December 30st, 2011.Multiplex RT-PCR was performed to detect 16 respiratory viral pathogens.Results: 279 patients were admitted for ARIs.The patients aged from 1 month to 12 years, with the median age of 2 years.Of which, 105 (37.6%) were positive for at least one pathogen.A total of 136 respiratory viral pathogens were identified from the 105 patients.Respiratory syncytial virus (RSV) was the most frequently detected pathogen (26.5%, 36/136), followed by parainfluenza virus (PIV) 1-3 (22.1%, 30/136), human rhinovirus (HRV) (21.3%, 29/136), human coronavirus (CoV) (10.3%, 14/136) and human adenovirus (HAdV) (9.6%, 13/136).Influenza A (Flu A), human metapneumovirus (hMPV) and human bocavirus (BoCA) were found 4.4%, 3.7% and 2.2%, respectively.Influenza B (Flu B) and seasonal influenza A H1N1(sH1N1) were not detected.Single-infections were detected in 30.5% (85/279) of cases.RSV was the most common pathogens in patients under 1 year and showed seasonal variation with peaks during winter and spring.Conclusions: This paper presents data on the epidemiology of viral pathogens associated with ARIs among children in Gansu Province, China.RSV is most frequently detected in our study.The findings could serve as a reference for local CDC in drawing up further plans to prevent and control ARIs.

10.1371/annotation/91d141f8-549c-475c-891a-5d8b4e5f91fd

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The complete genome sequence of Bifidobacterium longum LTBL16, a potential probiotic strain from healthy centenarians with strong antioxidant activity

Genomics (2019)

Guohong Huang Haibo Pan Zhenjun Zhu Quanyang Li

Bifidobacterium longum

10.1016/j.ygeno.2019.05.015

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Citations (28)

Analysis of peripheral blood lymphocyte subsets in multiple myeloma patients with different immunophenotypes

Zhongliu yanjiu yu linchuang (2018)

Guohong Huang Zhiwei Li

Objective To explore the clinical significance of peripheral blood lymphocytesubsets detection in multiple myeloma (MM) patients with different immunophenotypes. Methods A total of 40 MM patients in People's Hospital of Xinjiang Uygur Autonomous Region from January 2016 to June 2016 were selected as the research objects, and 20 healthy examinations were treated as the control group. Marrow and blood samples were collected to detect the expressions of antigens and peripheral blood lymphocytesubsets by using flow cytometry. Besides, immunophenotype and the differences of lymphocytesubsets between the two groups were analyzed. Results The expression rates of CD138, CD38, CD56, CD19, CD20, CD45 were 100% (40/40), 100% (40/40), 75% (30/40), 0, 20% (8/40), 0 in MM group and 100% (20/20), 100% (20/20), 0, 100% (20/20), 100% (20/20), 75% (15/20) in the control group. There were two kinds of immunophenotypes in MM group including CD138+ CD38+ CD56- CD19- (10 cases) and CD138+ CD38+ CD56+ CD19- (30 cases). The absolute value of CD3+ [(924±341)/ml vs. (1 474±270)/ml], CD4+ [(410±163)/ml vs. (871±214)/ml], CD4+/CD8+ [(1.0±0.3)/ml vs. (1.6±0.5)/ml], CD19+ [(72±26)/ml vs. (277±89)/ml] was lower in MM group compared with the control group(P 0.05). Conclusions Flow cytometry could help to type accurately the different immunophenotypes, distinguish the benign and malignant of plasmocytes and assist the diagnosis for the MM patients. Peripheral blood lymphocytesubsets analysis can be used to reflect the immunity of MM patients. Key words: Multiple myeloma; Immunophenotyping; Lymphocyte subsets; Flow cytometry

Immunophenotyping

Cytometry

Peripheral blood lymphocyte

10.3760/cma.j.issn.1006-9801.2018.03.008

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Study on the characteristic of anti-HLA antibody produced by hemodialysis in renal transplantation patients before operation

Chin J Clin Lab Mgt (Electronic Edition) (2019)

Bo Dai Guohong Huang Jie Zhuang Lijuan Wu Li Wang

Objective To understand the distribution and probability of anti-human leukocyte antigen (anti-HLA) antibody produced by hemodialysis before renal transplantation. Methods A total of 169 serum samples were collected from January 2015 to December 2017, from patients without sensitization history before kidney transplantation in our hospital. Anti-HLA antibodies were detected using Luminex 200 detection platform and corresponding reagents, and then the data was analyzed. Results 64.50% (109/169) of the 169 hemodialysis patients produced anti-HLA antibody. With the prolongation of hemodialysis time, the positive rate of anti-HLA antibody in patients′ serum increased, but the difference was not significant (χ2=6.009, P>0.05). 186 anti-HLA ClassⅠantibody and 158 ClassⅡantibody detected. Among the anti-HLA ClassⅠantibody, the highest frequency antibody were anti-A11, A25, A66, A80, B8, B75, B76, and Cw17. Among anti-HLA ClassⅡ antibody, anti-DR4, DR51, DR52, DR53, DQ2, DQ4, DQ6, DQ7, DP1, DP5, DP11 and DP13 were higher. Conclusions Anti-HLA antibody can be produced by patients with non sensitized renal transplantation after hemodialysis. Hemodialysis may be another factor of sensitization, which should be paid attention to in clinic. Key words: Kidney transplantation; Hemodialysis; Anti-HLA antibody; Sensitization

Panel reactive antibody

10.3877/cma.j.issn.2095-5820.2019.04.004

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[Construction of NF-kappaB siRNA expression vector].

Kurexi Yunusi Guohong Huang Mei Long Jie He Maimaitiaili Nasier

Objective To construct a NF-kappaB siRNA expression vector and to detect the specific silencing effect of the siRNA on the expression of NF-kappaB protein. Methods pcDNA3.1/CT-GFP-TOPO recombinant eukaryotic expression vector and pSilencer 1.0-U6-siRNA-NF-kappaB recombinant vector were constructed respectively. These 2 recombination plasmids were co-transfected into COS-7 cells, and the NF-kappaB silence induced by RNAi was detected by Western blot and the inverted fluorescence microscope. Results The levels of NF-kappaB protein in COS-7 cells could be silenced effectively and specifically by pSilencer 1.0-U6-siRNA- NF-kappa recombinant vector. The expression of NF-kappaB protein was reduced gradually with the increase of pSilencer 1.0-U6-siRNA- NF-kappaB recombinant vector,which could be detected by Western blot under the inverted fluorescence microscope. Conclusion NF-kappaB siRNA expression vector is constructed successfully.

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Transmission of Human Enterovirus 85 Recombinants Containing New Unknown Serotype HEV-B Donor Sequences in Xinjiang Uighur Autonomous Region, China

PLoS ONE (2013)

Qiang Sun Yong Zhang Shuangli Zhu Huifang Tian Guohong Huang

Background Human enterovirus 85 (HEV85), whose prototype strain (Strain BAN00-10353/BAN/2000) was isolated in Bangladesh in 2000, is a recently identified serotype within the human enterovirus B (HEV-B) species. At present, only one nucleotide sequence of HEV85 (the complete genome sequence of the prototype strain) is available in the GenBank database. Principal Findings In this study, we report the genetic characteristics of 33 HEV85 isolates that circulated in the Xinjiang Uighur autonomous region of China in 2011. Sequence analysis revealed that all these Chinese HEV85 isolates belong to 2 transmission chains, and intertypic recombination was found with the new unknown serotype HEV-B donor sequences. Two HEV85 isolates recovered from a patient presenting acute flaccid paralysis and one of his contacts were temperature-insensitive strains, and some nucleotide substitutions in the non-coding regions and in the 2C or 3D coding regions may have affected the temperature sensitivity of HEV85 strains. Conclusions The Chinese HEV85 recombinant described in this study trapped a new unknown serotype HEV-B donor sequence, indicating that new unknown HEV-B serotypes exist or circulate in Xinjiang of China. Our study also indicated that HEV85 is a prevalent and common enterovirus serotype in Xinjiang.

Coding region

Coxsackievirus

Strain (injury)

10.1371/journal.pone.0055480

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Citations (21)

Predictive value of von Willebrand factor for venous thrombosis in patients with chronic heart failure complicated with atrial fibrillation after anticoagulant therapy

BMC Cardiovascular Disorders (2023)

Jinping Song Yuan Liu Guohong Huang

We investigated the value of von Willebrand factor (vWF) in predicting venous thrombosis in patients with chronic heart failure complicated with atrial fibrillation after anticoagulation therapy.Totally, 126 patients with chronic heart failure complicated with atrial fibrillation who were treated with anticoagulant therapy and 60 healthy individuals were enrolled. One year after anticoagulant therapy, venous thrombosis occurred in 19 patients. Clinical data of patients were collected. The plasma vWF activity was detected and compared. The logistic regression analysis was used to analyze the influencing factors of vWF. ROC curve was used to evaluate the predictive value of plasma vWF.Plasma vWF activity was significantly higher in patients with heart failure and atrial fibrillation than control subjects (P < 0.01). The vWF activity in patients with venous thrombosis was significantly higher than that in patients without venous thrombosis (P < 0.01). ROC curve analysis showed that the cut-off value of vWF activity for venous thrombosis within one year after anticoagulant therapy was 267.5%, and the AUC was 0.742 (95% CI: 0.764-0.921, P < 0.05). The sensitivity was 80.0%, and the specificity was 63.6%. Factors of diabetes, myocardial ischemia, old myocardial infarction, and lower extremity atherosclerosis, but not sex, age, coronary heart disease, hypertension, and cardiac function, had significant effect on vWF activity (P < 0.05). Logistic regression analysis showed that vWF activity was significantly related with atherosclerosis of lower limbs and old myocardial infarction, but not significantly related with diabetes and myocardial ischemia. The risk of venous thrombosis in patients with vWF activity greater than 267.5% was 10.667 times higher than that in patients with vWF activity less than 267.5% (P < 0.05).The vWF activity greater than 267.5% has clinical predictive value for the risk of lower extremity venous thrombosis in patients with chronic heart failure complicated with atrial fibrillation within 1 year of anticoagulant therapy.

10.1186/s12872-023-03167-1

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An outbreak following importation of wild poliovirus in Xinjiang Uyghur Autonomous Region, China, 2011

BMC Infectious Diseases (2015)

Haibo Wang Wenzhou Yu Xinqi Wang Fuerhati Wushouer Jianping Wang

After more than 10 years without a case of wild poliovirus (WPV) in China, an outbreak occurred in 2011 in Xinjiang Uyghur Autonomous Region. Acute flaccid paralysis (AFP) case surveillance was strengthened with epidemiological investigations and specimen collection and serological surveys were conducted among hospitalized patients. There were 21 WPV cases and 23 clinical compatible polio cases reported. WPV was isolated from 14 contacts of AFP cases and 13 in the healthy population. Incidence of WPV and clinical compatible polio cases were both highest among children <1 years, however, 24/44 (54.5%) polio cases were reported among adults aged 15–39 years. High coverage of routine immunization should be maintained among children until WPV transmission is globally eradicated. Expansion of AFP case surveillance and use of serologic surveys to estimate population immunity should be conducted rapidly to guide preparedness and response planning for future WPV outbreaks.

Herd Immunity

Medical microbiology

Preparedness

10.1186/s12879-015-0761-y

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Citations (9)

Outbreak of Epidemic Keratoconjunctivitis Caused by Human Adenovirus Type 56, China, 2012

PLoS ONE (2014)

Guohong Huang Wenqing Yao Wei Yu Lingling Mao Haibo Sun

HAdV-56 is a new recombinant type isolated from epidemic keratoconjunctivitis (EKC) patients and has been sporadically isolated in Japan several times. Here, an outbreak of EKC in the city of Dalian, China involving a large number of workers in two factories was reported; this was the first outbreak of EKC associated with HAdV-56 worldwide.

Keratoconjunctivitis

10.1371/journal.pone.0110781

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Citations (43)

Respective and interactive effects of doubled CO2 and O3 concentration on membrane lipid peroxidation and antioxidative ability of soybean

Science in China Series C Life Sciences (2005)

Tianhong Zhao Yi Shi Guohong Huang Wang Yan Sun Bei

Malondialdehyde

10.1007/bf02889811

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Citations (5)