Sid Ahmed Rebiahi

University of Abou Bekr Belkaïd

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Ahlam Fatmi

Université de M'Sila

José Santiago Ibáñez-Cabellos

Parc Científic de la Universitat de València

Hafsa Azzaoui

University of Abou Bekr Belkaïd

Federico V. Pallardó

Centre for Biomedical Network Research on Rare Diseases

Yamina Elhabiri

University of Abou Bekr Belkaïd

José Luis García‐Gimenez

Centre for Biomedical Network Research on Rare Diseases

Mourad Aribi

Dynamic Systems (United States)

Souheila Benmassour

University of Abou Bekr Belkaïd

Nafissa Chabni

Dynamic Systems (United States)

Hanane Zerrouki

Czech Academy of Sciences, Institute of Microbiology

Cooperative Institutions

University of Abou Bekr Belkaïd

INCLIVA Health Research Institute

Centre for Biomedical Network Research on Rare Diseases

Dynamic Systems (United States)

Parc Científic de la Universitat de València

Universitat de València

Instituto de Salud Carlos III

Centro de Investigación Biomédica en Red

Université de ain Témouchent

Menéndez Pelayo International University

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miRNA-23b as a biomarker of culture-positive neonatal sepsis 

Research Square (Research Square) (2020)

Ahlam Fatmi Sid Ahmed Rebiahi Nafissa Chabni Hanane Zerrouki Hafsa Azzaoui

Abstract Background: Neonatal sepsis remains an important cause of morbidity and mortality. The ability to quickly and accurately diagnose neonatal sepsis based on clinical assessments and laboratory blood tests remains difficult, where haemoculture is the gold standard for detecting bacterial sepsis in blood culture. It is also very difficult to study because neonatal samples are lacking. Methods : Forty-eight newborns suspected of sepsis admitted to the Neonatology Department of the Mother-Child Specialized Hospital of Tlemcen. From each newborn, a minimum of 1-2 ml of blood was drawn by standard sterile procedures for blood culture. The miRNA-23b level in haemoculture was evaluated by RT-qPCR. Results : miR-23b levels increased in premature and full-term newborns in early onset sepsis ( p < 0.001 and p < 0.005 respectively), but lowered in late onset sepsis in full-term neonates ( p < 0.05) compared to the respective negative controls. miR-23b levels also increased in late sepsis in the negative versus early sepsis negative controls ( p < 0.05). miR-23b levels significantly lowered in the newborns who died from both sepsis types ( p < 0.0001 and p < 0.05 respectively). In early sepsis, miR-23b and death strongly and negatively correlated (correlation coefficient = -0.96, p = 0.0019). In late sepsis, miRNA-23b and number of survivors (correlation coefficient = 0.70, p = 0.506) positively correlated. Conclusions : Lowering miR-23b levels is an important factor that favours sepsis development, which would confirm their vital protective role, and strongly suggest that they act as a good marker in molecular diagnosis and patient monitoring.

Neonatal Sepsis

Neonatology

Blood Culture

Positive correlation

10.21203/rs.3.rs-23232/v3

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Citations (1)

mir-23b: A New Molecular Marker For Neonatal Sepsis In Haemoculture

Research Square (Research Square) (2020)

Ahlam Fatmi Sid Ahmed Rebiahi Nafissa Chabni Hanane Zerrouki Hafsa Azzaoui

Abstract Background: Neonatal sepsis remains an important cause of morbidity and mortality. The ability to quickly and accurately diagnose neonatal sepsis based on clinical assessments and laboratory blood tests remains difficult, where haemoculture is the gold standard for detecting bacterial sepsis in blood culture. It is also very difficult to study because neonatal samples are lacking. Methods : Fifty-four newborns suspected of sepsis admitted to the Neonatology Department of the Mother-Child Specialized Hospital of Tlemcen. From each newborn, a minimum of 1-2 ml of blood was drawn by standard sterile procedures for blood culture. The miRNA-23b level in haemoculture was evaluated by RT-qPCR. Results : miR-23b levels increased in premature and full-term newborns in early onset sepsis ( p < 0.001 and p < 0.005 respectively), but lowered in late onset sepsis in premature and full-term neonates ( p < 0.005) compared to the respective negative controls. miR-23b levels also increased in late sepsis negative versus early sepsis negative controls ( p < 0.05). miR-23b levels significantly lowered in the newborns who died from both sepsis types ( p < 0.0001 and p < 0.05 respectively). Conclusions : The drop in miR-23b levels is an important factor that favours sepsis development, which would confirm their vital protective role, and strongly suggest that they act as a good marker in both molecular diagnosis and patient monitoring.

Neonatal Sepsis

Neonatology

Blood Culture

Gold standard (test)

10.21203/rs.3.rs-23232/v1

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Citations (0)

Evaluation of Tetraclinis articulata essential oil from Algeria flora as a potential source of antifungal activity and study of its chemical composition

Journal of the Indian Academy of Wood Science (2013)

Fatima Zahra Abi-Ayad Meryem Abi-Ayad Hammadi A. Lazouni Sid Ahmed Rebiahi

Aspergillus niger

Germacrene D

10.1007/s13196-013-0086-7

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Citations (11)

CARACTERISATION DE SOUCHES DE Staphylococcus aureus ET ETUDE DE LEUR ANTIBIORESISTANCE AU NIVEAU DU CENTRE HOSPITALO-UNIVERSITAIRE DE TLEMCEN

Sid Ahmed Rebiahi

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Antimicrobial Resistance Profiles and Molecular Characterisation of Vancomycin Resistance Genes in Enterococcus spp in Algeria

JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH (2020)

Malika Rahmoun Sid Ahmed Rebiahi Hafida Hassaïne Zakaria Boutarfi Leila Soufi

Introduction: The vanA gene continues to spread throughout the world. Algeria does not seem to be spared, but the data, which remain sporadic, are also old. This has justified the overriding interest in exploring the current state of antibiotic resistance in Enterococci, while focusing on the presence of certain genes. Aim: To study the isolation frequency and the level of antibiotic resistance of Enterococcus faecalis (E. faecalis) and Enterococcus faecium (E. faecium) isolated during two years at the Tlemcen Hospital (northwest Algeria), while investigating the possible presence of Vancomycin Resistant Enterococci (VRE). Materials and Methods: The present study was a prospective study in which Enterococcus spp was isolated from five different departments which were identified and confirmed by molecular identification with ‘tuf’ gene. Antibiotic sensitivity was done by the agar diffusion and Minimum Inhibitory Concentration (MIC) method. The vancomycin resistance genes (van A, van B) were researched by Polymerase Chain Reaction (PCR) and then sequenced by the Genoscreen laboratory in Lille (France). SPSS software version 20 (IBM Statistical Package for the Social Sciences (SPSS) statistics 20) was used to analyse the data obtained from the study. Results: The PCR of the “tuf” gene revealed two predominant species E. faecalis and E. faecium. All isolates have a multidrug resistance, two E. faecium were distinguished by their resistance to vancomycin with MICs >256 μg/mL. At the origin of this resistance, the vanA gene was characterised and sequenced; the obtained sequence has been introduced into the Genbank National Center for Biotechnology Information (NCBI) database. Conclusion: This work revealed alarming levels of antibiotic resistance in Enterococci, the vanA gene was found in two E. faecium; sequencing of this gene has revealed a total homology with another isolated in Cuba, which demonstrates a worldwide spread of this resistance gene.

Enterococcus faecalis

Enterococcus faecium

10.7860/jcdr/2020/44289.14068

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Citations (0)

miRNA-23b as a biomarker of culture-positive neonatal sepsis

Molecular Medicine (2020)

Ahlam Fatmi Sid Ahmed Rebiahi Nafissa Chabni Hanane Zerrouki Hafsa Azzaoui

Abstract Background Neonatal sepsis remains an important cause of morbidity and mortality. The ability to quickly and accurately diagnose neonatal sepsis based on clinical assessments and laboratory blood tests remains difficult, where haemoculture is the gold standard for detecting bacterial sepsis in blood culture. It is also very difficult to study because neonatal samples are lacking. Methods Forty-eight newborns suspected of sepsis admitted to the Neonatology Department of the Mother-Child Specialized Hospital of Tlemcen. From each newborn, a minimum of 1–2 ml of blood was drawn by standard sterile procedures for blood culture. The miRNA-23b level in haemoculture was evaluated by RT-qPCR. Results miR-23b levels increased in premature and full-term newborns in early onset sepsis ( p < 0.001 and p < 0.005 respectively), but lowered in late onset sepsis in full-term neonates ( p < 0.05) compared to the respective negative controls. miR-23b levels also increased in late sepsis in the negative versus early sepsis negative controls ( p < 0.05). miR-23b levels significantly lowered in the newborns who died from both sepsis types ( p < 0.0001 and p < 0.05 respectively). In early sepsis, miR-23b and death strongly and negatively correlated (correlation coefficient = − 0.96, p = 0.0019). In late sepsis, miRNA-23b and number of survivors (correlation coefficient = 0.70, p = 0.506) positively correlated. Conclusions Lowering miR-23b levels is an important factor that favours sepsis development, which would confirm their vital protective role, and strongly suggest that they act as a good marker in molecular diagnosis and patient monitoring.

Neonatal Sepsis

Neonatology

Blood Culture

Positive correlation

10.1186/s10020-020-00217-8

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Citations (23)

Correction to: miRNA-23b as a biomarker of culture-positive neonatal sepsis

Molecular Medicine (2020)

Ahlam Fatmi Sid Ahmed Rebiahi Nafissa Chabni Hanane Zerrouki Hafsa Azzaoui

An amendment to this paper has been published and can be accessed via the original article.

Neonatal Sepsis

10.1186/s10020-020-00257-0

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Citations (2)

Efficiency study of biocides used in hospitals on Enterococci

Genetics and Biodiversity Journal (2019)

Sid Ahmed Rebiahi Malika Rahmoun Wafa Khazini Meriem Kaouadji Amira Mahi

The present work explore the efficiency of some disinfectants used in hospitals in the operating theatersof the Maternity Department and the Neonatology Unit in the EHS of Tlemcen. Four biocides ofdifferent formulations were used: quaternary ammonium, glutaraldehyde, alcohols, and quaternaryammonium + alcohols. The disinfectant sensitivity test was carried out by determining the minimuminhibitory concentration of the different molecules on microplate with respect to eight reference strainsand 50 strains of Enterococci isolated in a hospital environment. The results of the MIC revealed agood efficiency of quaternary ammoniums and alcohols on Enterococci. However, the glutaraldehydeproduct remained ineffective with respect to the majority of strains tested. In conclusion, theconcentration of the disinfectant used the diversity of the active ingredient and the type ofmicroorganism greatly influence the activity of the biocide.

Disinfectant

Biocide

Glutaraldehyde

Vancomycin-Resistant Enterococci

10.46325/gabj.v3i1.52

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Citations (0)

miRNA-23b as a biomarker of culture-positive neonatal sepsis

Research Square (Research Square) (2020)

Ahlam Fatmi Sid Ahmed Rebiahi Nafissa Chabni Hanane Zerrouki Hafsa Azzaoui

Neonatal Sepsis

Blood Culture