With the rapid development of tissue engineering and gene therapy, collagen-based biomaterials are frequently used as cell transplant devices; an example is bio-artificial skin substitutes [1-3]. In this study, we determined the effects of two different cell-seeding methods, monolayer and suspension seeding, on the rate of contraction of free-floating collagen and collagenglycosaminoglycan (GAG) gel matrices in vitro. 3T3 mouse fibroblasts were cultured in/on the matrices for up to 7 days.

3T3 cells

Artificial skin

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Influence of age on postischaemic reactive hyperaemia

Clinical Physics and Physiological Measurement (1991)

S Hagisawa J.C. Barbenel R M Kenedi

The authors' work was carried out to evaluate the response of skin microcirculation to ischaemia in younger and older subjects, to determine if there were functional changes in microcirculation in the older skin. Skin perfusion during reactive hyperaemia was measured a volar sites on the forearms. Ischaemia was produced by the application of loads applied to the skin surface for 3 min by means of a hollow cylindrical indentor. The skin perfusion within the area indented was continuously monitored by a laser Doppler flowmeter before, during and after loading. In the pre-loaded condition, there was no difference in skin perfusion between the younger and the older groups. Following loading with 22.3 N a significantly lower value of the peak perfusion relative to the pre-loaded value was observed in the older group. The perfusion in the older group following loading with 44.5 N was significantly reduced compared to the younger group. It is concluded that postischaemic reactive hyperaemia in the older skin was reduced compared with the younger skin.

Hyperaemia

Reactive hyperemia

10.1088/0143-0815/12/3/003

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The thermal properties of skin

Springer eBooks (1988)

J.C. Barbenel

10.1007/978-94-009-1291-5_20

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The variability of skin surface contours