Development and study of novel biomimetic and biocompatible nano-engineered functional nanofilm
materials are currently important from fundamental and applied viewpoints [1]. Such nanomaterials can
serve as model systems for insight into the basic structural-functional interconnections at the nano-scale in
biological membranes and are useful for bio-medical applications including controlled drug delivery.
We present results of a study of novel nanofilm structures (Langmuir monolayers and Langmuir-Blodgett
films) formed by functional amphiphilic amines and their interfacial complexes with colloid nanoparticles
and polyelectrolytes including DNA. It was found that stearoylspermine molecules form stable Langmuir
monolayer on an aqueous subphase surface. The stearylspermine Langmuir monolayer compression
isotherm changes caused by interactions of the monolayer with aqueous subphase components (colloid
ligand-free magnetite nanoparticles and DNA molecules) have been studied. The observed characteristic
changes in Langmuir monolayer compression isotherms indicated to the binding of nanoparticles and
polyanions with cationic stearoylspermine monolayer. Monolayer LB films of interfacial polycomplexes
formed by stearylspermine and magnetite nanoparticles or DNA molecules were deposited onto the mica
substrate surface and the structure of obtained films was investigated using AFM technique.
The Langmuir monolayers and deposited LB films of the other type of functional amine-containing
compounds which are pH-sensitive synthetic lipids have been also studied. In our work the pH-sensitive
lipid trans-4,5,didodecyloxycarbonyl- trans-2-morpholinocyclohexanol was synthesized and studied. We
have found the substantial changes of the Langmuir monolayer compression isotherm shape of that lipid
caused by the change in aqueous subphase pH value from 7.5 to 5. Those changes can be the result of pH-
induced conformational changes in the pH-sensitive lipid molecule. Corresponding monolayer LB films were
deposited on mica substrate and studied using AFM technique. The various pH-dependent structural
features including surface micelles were observed in the LB films deposited at various pH subphase values.
The investigated functional amine-containing compounds are promising candidates for many potential
applications including formation of liposomal vesicles for encapsulation and controlled release, DNA and
drug delivery, formation of functional coatings on various substrate surfaces, etc.
To create a molecular transistor, that is capable to operate in single-electron regime at room temperature, nanogap of several nanometers between electrodes is needed. Such nanogaps can be obtained by electromigration. In this work the technique of the creation of nanowire samples suitable for electromigration is described. Nanowires were formed on a substrate without buffer metallic layer to provide best conditions for electromigration process.
The present paper concerns a planar topology for a nanoelectronic transducer based on the glucose oxydase enzyme. The technique of implementation of planar nanostructures for a nanoelectronic biosensor based on the methods of electron-beam lithography is developed. The method of the chemical modification of silicon dioxide surface by epoxysilane was developed and the immobilization of the glucose oxydase enzyme on the surface of planar nanostructure for the nanoelectronic transducer through the linker molecules was realized. The registration procedure of the biochemical signals was developed. A change in the functional state of the immobilized ferment of the glucose oxidase enzyme with the presence of glucose in the test solution was demonstrated.
The transport characteristics of a single-electron transistor based on molecules with a monatomic charge center based on Rh, Ru, Pt, Sc, Cr atoms are calculated. The energy spectra of the studied molecules in various charge states are calculated. The results of the calculation of the parametric model's coefficients of molecular shells for the studied molecules are presented.
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