Shirley Longacre

Institut Pasteur

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Inge Holm

Institut Pasteur

Harvey Eisen

University of Washington

Anna Raibaud

Institut Pasteur

Ronald Perraut

Centre Pasteur du Cameroun

Odile Mercereau‐Puijalon

Modèles Insectes de l'Immunité Innée

Adama Tall

Institut Pasteur de Dakar

Urszula Hibner

Institut de Génétique Moléculaire de Montpellier

G.A. Bentley

Institut Pasteur

Preethi V. Udagama-Randeniya

University of Colombo

Gregory A. Buck

Virginia Commonwealth University

Cooperative Institutions

Institut Pasteur

Centre National de la Recherche Scientifique

Inserm

Université Paris Cité

Sorbonne Université

Institut Pasteur de Dakar

Institut de Recherche pour le Développement

University of Colombo

Sorbonne Paris Cité

Mère et Enfant en Milieu Tropical

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In vitro responses of senegalese adults PBMC to recombinant MSP1 19 antigens depends on expression systems and/or Plasmodium falciparum exposure

American Journal of Tropical Medicine and Hygiene (1996)

O. Garraud Ababacar Diouf Shirley Longacre David C. Kaslow Anthony A. Holder

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Gene polymorphism of Plasmodium falciparum merozoite surface proteins 4 and 5

Molecular and Biochemical Parasitology (2005)

Hannah E.J. Polson David J. Conway Thierry Fandeur Odile Mercereau‐Puijalon Shirley Longacre

Merozoite surface protein

Surface protein

10.1016/j.molbiopara.2005.02.009

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Citations (23)

Antigenic Variation in African Trypanosomes

Springer eBooks (1984)

Harvey Eisen Shirley Longacre Gregory A. Buck

Antigenic variation

clone (Java method)

10.1007/978-3-642-70065-1_5

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Different Plasmodium falciparum Recombinant MSP1₁₉ Antigens Differ in Their Capacities to Stimulate In Vitro Peripheral Blood T Lymphocytes in Individuals from Various Endemic Areas

Scandinavian Journal of Immunology (1999)

Olivier Garraud Ababacar Diouf Cheikh Momar Nguer Alioune Dièye Shirley Longacre

This study reports on T‐cell proliferative responses to the 19‐kDa C‐terminal domain of the Plasmodium falciparum merozoite surface protein (MSP1 19 ). Three different recombinant proteins were used: an Escherichia coli product expressing the first EGF‐like domain and Saccharomyces cerevisiae and baculovirus/insect‐cell‐produced proteins containing both EGF‐like domains, the latter protein being produced with or without N ‐glycosylation. Cell donors were P. falciparum ‐immune adults with no recent history of clinical malaria and recruited from three Senegalese settings with different epidemiological parasite transmission. Each mononuclear‐blood‐cell preparation was stimulated with a range of concentrations of the three proteins. Most subjects’ mononuclear cells were reactive to at least one protein, but significant differences in lymphoproliferation were seen between the settings and within individual cultures depending on the protein source and concentration. Importantly, lymphoproliferation indices correlated inversely with the intensity of P. falciparum malaria transmission. When purified T lymphocytes were cultured in the presence of MSP1 19 plus autologous monocytes, B lymphocytes or a proposed CD1 + dendritic‐cell population as costimulatory cells, significant differences were observed depending on the individual's previous exposure to parasites. This study shows that the stimulation of lymphocyte proliferation in vitro with MSP1 19 depends on several factors, including epidemiological conditions and protein preparations.

10.1046/j.1365-3083.1999.00511.x

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A precise quantitation of gene number by saturation hybridization using cloned DNA

Nucleic Acids Research (1979)

Shirley Longacre Bernard Mach

This paper describes a precise method of gene titration as applied to the alpha- and beta-globin genes in the mouse. The three salient features of the method are: (i) the use of saturation hybridization in probe cDNA excess, (ii) the use of highly purified cDNA probes prepared by preparative hybridization with cloned globin sequences (Longacre and Mach (1978) J. Biol. Chem. 253, 7500) and (iii) the use of cloned globin sequences to calibrate the system internally. The results indicate that there are two genes for alpha-globin and two genes for beta-globin in the BALB/c mouse. The significance of these results are discussed in relation to other data regarding adult and embryonic globin genes.

Hybridization probe

10.1093/nar/6.4.1241

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Citations (7)

Influence of strain differences in mice on the metabolism and toxicity of benzene*1, *2

Toxicology and Applied Pharmacology (1981)

Shirley Longacre

Glucuronide

10.1016/0041-008x(81)90324-0

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Citations (74)

Splicing Intermediates of the IL-2 Receptor (p55 Subunit)

De Gruyter eBooks (1988)

Patrick Froussard Patricia Chastagner Jacques Thèze Shirley Longacre

10.1515/9783110850253-033

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The Plasmodium cynomolgi merozoite surface protein 1 C-terminal sequence and its homologies with other Plasmodium species

Molecular and Biochemical Parasitology (1995)

Shirley Longacre

Merozoite surface protein

Plasmodium (life cycle)

Sequence (biology)

Surface protein

10.1016/0166-6851(95)02477-8

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Citations (21)

DNA Rearrangements and Antigenic Variation in Trypanosoma equiperdum: Multiple Expression-Linked Sites in Independent Isolates of Trypanosomes Expressing the Same Antigen

Molecular and Cellular Biology (1983)

Shirley Longacre Urszula Hibner Anna Raibaud Harvey Eisen Théo Baltz

African trypanosomes resist the immune response of their mammalian hosts by varying the surface glycoprotein which constitutes their antigenic identity. The molecular mechanism of this antigenic variation involves the successive activation of a series of genes which code for different variant surface glycoproteins (VSGs). We have studied the expression of two VSG genes (those of VSG-1 and VSG-28) in Trypanosoma equiperdum, and we report the following findings. (i) The expression of both VSG genes is associated with the duplication and transposition of corresponding basic copy genes. (ii) The duplicated transposed copy appears to be the expressed copy. (iii) Although there are multiple genes which cross-hybridize with the VSG-1 cDNA probe, only one of these appears to be used as a template for the expression-linked copy in four independent BoTat-1 clones. (iv) Analysis of the genomic environments of the expressed VSG-1 genes from each of four independently derived BoTat-1 trypanosome clones revealed that there are at least three different sites into which the expression-linked copy can be inserted.

Antigenic variation

10.1128/mcb.3.3.399-409.1983

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Citations (18)

Induction of P. falciparum antigen-specific T and B cell responses to MSP1 p19 in naive donors after priming in vitro

Immunology Letters (1997)

Olivier Garraud Inge Holm Ababacar Diouf Cheikh Momar Nguer Shirley Longacre

Priming (agriculture)

10.1016/s0165-2478(97)86718-1

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