Kyoko Uchiyama

Institute of Livestock and Grassland Science

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Masashi Kinukawa

Institute of Livestock and Grassland Science

Yousuke Naniwa

Institute of Livestock and Grassland Science

Syohei Toda

Institute of Livestock and Grassland Science

Yoshiaki Minato

Institute of Livestock and Grassland Science

Olaf Erik Herrmann

Denso (Germany)

K. Kurogi

Institute of Livestock and Grassland Science

Kenji Togashi

Mie University

Atsushi Ogino

Institute of Livestock and Grassland Science

Katsuhiko Takeuchi

National Institute of Advanced Industrial Science and Technology

Masahiko Nakagawa

Hokkaido Research Organization

Cooperative Institutions

Institute of Livestock and Grassland Science

Denso (United States)

Hokkaido University

Tokyo University of Agriculture and Technology

National Agriculture and Food Research Organization

Tohoku University

The University of Tokyo

Shinshu University

Gifu University

Tokyo University of Agriculture

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Evaluation of sperm DNA damage in bulls by TUNEL assay as a parameter of semen quality

Journal of Reproduction and Development (2015)

Kumiko Takeda Kyoko Uchiyama Masashi Kinukawa Takahiro Tagami Masahiro Kaneda

Sperm DNA damage affects the conception rate resulting from human assisted reproduction technology. The objective of this study was to adapt the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay to provide a quality parameter for bull semen based on the detection of sperm DNA damage. Fresh semen was collected from two Japanese Black bulls (A, B) several times over the course of a year, and the percentage of TUNEL-positive spermatozoa (sperm TUNEL index) was determined. Individual differences in semen were detected using the sperm TUNEL index in these bulls (P < 0.01). The sperm TUNEL index of cryopreserved semen obtained from test-mated Japanese Black (n = 30, including two bulls with a conception rate lower than 10%) and Holstein (n = 34) bulls were analyzed. The average sperm TUNEL index and conception rate resulting from artificial insemination (AI) were 4.7% and 55.7% for Japanese Black, and 4.9% and 39.5% for Holstein, respectively. A weak negative correlation between sperm TUNEL index and conception rate was observed in Holstein bulls (P < 0.05). Semen samples from six bulls with more than 10% sperm TUNEL index were studied, and these samples showed low sperm viability. However, semen resulting in a very low conception rate did not have a high sperm TUNEL index. Although it would be difficult to predict a low conception rate resulting from AI using the sperm TUNEL index alone, the index can be used as an additional parameter to provide a more comprehensive description of semen quality.

Terminal deoxynucleotidyl transferase

Semen quality

Semen Analysis

10.1262/jrd.2014-140

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Citations (43)

Saturation magnetic moments of Co/Cr multilayers

Journal of Magnetism and Magnetic Materials (1996)

Kyoko Uchiyama I. Ishida E. Hirota K. Hamada Ayumi Okada

Saturation (graph theory)

10.1016/0304-8853(95)00926-4

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Autonomous Three-Dimensional Formation Flight for a Swarm of Unmanned Aerial Vehicles

Journal of Guidance Control and Dynamics (2011)

Derek J. Bennet Colin MacInnes Masayuki Suzuki Kyoko Uchiyama

This paper investigates the development of a new guidance algorithm for a formation of unmanned aerial vehicles.Using the new approach of bifurcating potential fields, it is shown that a formation of unmanned aerial vehicles can be successfully controlled such that verifiable autonomous patterns are achieved, with a simple parameter switch allowing for transitions between patterns.The key contribution that this paper presents is in the development of a new bounded bifurcating potential field that avoids saturating the vehicle actuators, which is essential for real or safety-critical applications.To demonstrate this, a guidance and control method is developed, based on a six-degreeof-freedom linearized aircraft model, showing that, in simulation, three-dimensional formation flight for a swarm of unmanned aerial vehicles can be achieved.

10.2514/1.53931

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Citations (68)

Estrous synchronization in swine by oral administration of altrenogest starting from proestrus or estrus.

Nihon Yoton Gakkaishi (1992)

Yoneto ITOH Yuri KONDO Kyoko Uchiyama Hiroshi MASUDA

合成黄体ホルモン剤 Altrenogest (AT) を発情前期～発情期 (Day19～Day2) と発情休止期 (Day3～Day18) から投与開始して, 発情同期化試験を行なった。試験は, 48頭の未経産豚および経産豚に, 1日1頭当たり20mgのATを経口投与した。試験Iでは, 11頭の未経産豚にDay19～Day2からAT投与を開始し, 5～8日間投与後屠殺して卵巣を検査した。試験IIでは, 37頭の未経産豚および経産豚を用い, 16頭にはDay19～Day2から, また21頭にはDay3～Day18からそれぞれ同量のATを18日間経口投与し, 発情同期化効果と同期化発情における受胎性について検討した。得られた結果は, 次のように要約される。1) 試験Iでは, 11頭中10頭において平均14.1±3.1個の黄体が形成され, 排卵が正常に起きたものと推測された。排卵が抑制されたものは1頭のみであった。2) 試験IIでは, Day19～Day2から投与開始し18日間投与した16頭中15頭 (93.8%) に, 投与終了後平均6.1±1.0日で発情が再帰し, 誘起された発情は平均48.0±13.6時間持続した。また, Day3～Day18から投与開始し18日間投与した21頭中20頭 (95.2%) に, 投与終了後平均6.1±1.2日で発情が再帰し, 誘起された発情の持続時間は平均60.0±22.0時間であり, 両群の間には有意差はみられなかった。3) Day19～Day2からAT投与を開始した群とDay3～Day18から投与開始した群における受精頭数の割合, 平均黄体数および受精卵率は, それぞれ80.0%と85.7%, 15.7±3.5と15.7±3.9個, 76.2%と76.1%で両群間に有意差はみられなかった。以上の結果より, ATを発情前期～発情期から投与開始した場合は, 発情休止期から投与開始した場合と同様に繁殖性を低下させることなく, 豚の発情を同期化できることが明らかになった。

10.5938/youton.29.152

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Comprehensive protein analysis of fresh and frozen-thawed bovine sperm by iTRAQ method

Masashi Kinukawa Kyoko Uchiyama Masahiko Ito

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L-amino acid oxidase 1 in sperm is associated with reproductive performance in male mice and bulls

Biology of Reproduction (2021)

Haolin Zhang Hong Liu Sachi Kataoka Masashi Kinukawa Kyoko Uchiyama

Abstract Sperm quality is an important indicator of male fertility, and a suitable biomarker enables the selection of high-quality spermatozoa. We previously found that L-amino acid oxidase encoded by the L-amino acid oxidase 1 (Lao1) gene exerts biological roles in the mammary gland and brain by converting specific L-amino acids into keto acids, ammonia, and hydrogen peroxide (H2O2). Here, we describe the role of Lao1 in male reproduction. Lao1-deficient (Lao1−/−) male mice generated fewer pregnant embryos and pups as well as lower ratios of fertilized oocytes and even ovulated number was not different, suggesting that male subfertility caused the smaller litters. We found that LAO1 expressed in acrosomes is associated with high malformation ratios and low viability of Lao1−/− sperm. Wild-type (WT) sperm produced more H2O2 than Lao1−/− sperm, and 10 μM H2O2 restored knockout (KO) sperm viability in vitro. In addition, the sperm ratio of induced acrosome reaction was higher in WT than in Lao1−/− sperm incubated with the calcium ionophore A23187. Moreover, LAO1 expression was abundant in bovine sperm with high fertilization ratios. We concluded that LAO1 localized in the sperm acrosome influences sperm viability and morphology as well as the acrosome reaction, and that LAO1-deficient sperm might cause male subfertility. Thus, LAO1 might serve as a novel marker for selecting high-quality spermatozoa, especially for livestock reproduction.

Acrosome reaction

10.1093/biolre/ioab024

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Comprehensive analysis of seminal plasma proteins in normal and subferile bulls by iTRAQ method

The Japanese Biochemical Society/The Molecular Biology Society of Japan (2017)

Masashi Kinukawa Yousuke Naniwa Kyoko Uchiyama Masahiko Ito

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Functional insulin‐like factor 3 (INSL3) hormone‐receptor system in the testes and spermatozoa of domestic ruminants and its potential as a predictor of sire fertility

Animal Science Journal (2016)

Ali Mohammed Pitia Kyoko Uchiyama Hiroaki Sano Masashi Kinukawa Yoshiaki Minato

Insulin-like factor 3 (INSL3) is essential for fetal testis descent, and has been implicated in the testicular and sperm functions in adult males; however, similar functions in domestic ruminants remain largely unknown. This study investigated the functional INSL3 hormone-receptor system in adult ruminant testes and spermatozoa, and explored its potential to diagnose the fertility of sires. Testes and spermatozoa were obtained from fertile bulls, rams and he-goats, whereas subfertile testes and spermatozoa were obtained only from bulls. As expected, INSL3 was visualized in Leydig cells, while we clearly demonstrated that the functional receptor, relaxin family peptide receptor 2 (RXFP2), enabling INSL3 to bind was identified in testicular germ cells and in the sperm equatorial segment of bulls, rams and he-goats. In comparison to fertile bulls, the percentage of INSL3- and RXFP2-expressing cells and their expression levels per cell were significantly reduced in the testes of subfertile bulls. In addition, the population of INSL3-binding spermatozoa was also significantly reduced in the semen of subfertile bulls. These results provide evidence for a functional INSL3 hormone-receptor system operating in ruminant testes and spermatozoa, and its potential to predict subfertility in sires.

Sire

10.1111/asj.12694

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Citations (19)

Innovative Technologieansätze zur Erreichung hoch effizienter Diesel Antriebssysteme mit niedrigsten Abgasemissionen /Innovative Technologies to Realize the Ultimate Diesel System with High Efficiency...

VDI Verlag eBooks (2017)

Masakazu Ito Katsuhiko Takeuchi Koji Ishizuka Kyoko Uchiyama S. Sugawara

10.51202/9783186802125-ii-255

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Bovine sperm sex‐selection technology in Japan

Reproductive Medicine and Biology (2018)

Yousuke Naniwa Yoshiya Sakamoto Syohei Toda Kyoko Uchiyama

Abstract Background In Japan, Livestock Improvement Association of Japan started commercially producing sexed bovine semen 10 years ago, and sexed bovine semen is currently used for the artificial insemination (AI) in the farms. In this review, the authors introduce the technology for sperm sexing by flow cytometry, the efforts at commercializing sexed semen in Japan, and recent field data on artificial insemination of the cattle with sexed semen. Methods In the procedures of the flow cytometric method, X‐chromosome‐bearing sperm and Y‐chromosome‐bearing sperm were fluorescently stained, separated from each other by analyzing the difference in the DNA content, and cryopreserved. The authors surveyed the conception rates after AI with these sperm and sex ratios of the offspring with the cooperation from livestock associations, AI technicians, and farmers. Main findings (Results) Although AI with sexed semen was associated with lower conception rates in comparison with AI with conventional semen, the accuracy of sex selection using AI with sexed semen was beyond >90%. Conclusion Sexed semen produced by flow cytometry has the potential to produce offspring of the preferred sex with high accuracy and reliability. Thus, it is expected that sexed semen is used for AI more frequently in the farms.

Sexing

Semen cryopreservation

10.1002/rmb2.12235

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Citations (17)