Qingping Luo

Hubei Academy of Agricultural Sciences

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Huabin Shao

Hubei Academy of Agricultural Sciences

Guoyuan Wen

Hubei Academy of Agricultural Sciences

Téngfēi Zhāng

Animal Science Research Institute

Qin Lu

Tongren Hospital

Hongcai Wang

Binzhou University

Yiluo Cheng

Hubei Academy of Agricultural Sciences

Zhe Zeng

Hubei Academy of Agricultural Sciences

Yu Shang

Hubei Academy of Agricultural Sciences

Yunqing Guo

Hubei Academy of Agricultural Sciences

Lun Yao

Hubei Academy of Agricultural Sciences

Cooperative Institutions

Hubei Academy of Agricultural Sciences

Animal Science Research Institute

Huazhong Agricultural University

Ministry of Agriculture and Rural Affairs

Chinese Academy of Sciences

Yangtze University

East China University of Science and Technology

Center of Hubei Cooperative Innovation for Emissions Trading System

Shanghai Zhangjiang Laboratory

Wuhan University

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An indirect sandwich ELISA for the detection of avian influenza H5 subtype viruses using anti-hemagglutinin protein monoclonal antibody

Veterinary Microbiology (2008)

Qingping Luo Hongliang Huang Wei Zou Hanbing Dan Xuebo Guo

Embryonated

Polyclonal antibodies

10.1016/j.vetmic.2008.12.009

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Citations (34)

Correlation between gyrA and CmeR Box Polymorphism and Fluoroquinolone Resistance in Campylobacter jejuni Isolates in China

Antimicrobial Agents and Chemotherapy (2017)

Téngfēi Zhāng Yiluo Cheng Qingping Luo Qin Lu Jun Dong

Sequence analysis of 79 ciprofloxacin-resistant Campylobacter jejuni isolates collected in China showed resistance-related sequence variations in gyrA and CmeR-Box. All the isolates contain an identical Thr-86-Ile substitution in GyrA. Several novel CmeR-Box variations, including point substitutions, deletion, and insertion, were identified. The point insertion or deletion led to dramatically reduced binding of CmeR to the cmeABC promoter, which significantly increases the expression of cmeABC and contributes to the high fluoroquinolone resistance.

Multilocus sequence typing

10.1128/aac.00422-17

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Citations (15)

Protection of chickens against fowl cholera by supernatant proteins of Pasteurella multocida cultured in an iron-restricted medium

Avian Pathology (2019)

Qingping Luo Ling‐Yan Kong Jun Dong Téngfēi Zhāng Honglin Wang

Pasteurella multocida (P. multocida), a causative agent of fowl cholera, is an important pathogen in the poultry industry. In the present study, we found that the inactivated vaccine of P. multocida grown in an iron-restricted medium provided better protection than that grown in normal medium. Thus, we adopted a comparative proteomics approach, by using two-dimensional gel electrophoresis (2-DE), coupled with matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/TOF MS), to profile the supernatant proteins associated with P. multocida under both conditions. Eleven upregulated proteins were identified, including aspartate ammonia-lyase (AspA), diacylglycerol kinase (DgK), 30S ribosomal protein S6 (RpsF), and eight outer membrane proteins (OMPs). To further characterize the three novel supernatant proteins identified under iron-restricted conditions, the AspA, DgK and RpsF proteins were expressed and purified, and used as immunogens to vaccinate chickens. The results showed that AspA, DgK and RpsF proteins induced 80.0%, 66.7%, and 80.0% immunity, respectively. These data indicate that the three novel proteins identified in the supernatant of the culture media might play important roles in the survival of bacteria under iron-restricted conditions, and thus protect chickens against P. multocida. These findings also suggest that the proteins identified can be used as subunit vaccines.

Fowl cholera

Fowl

Pasteurella

10.1080/03079457.2019.1568390

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Citations (6)

Construction and Bacteriostatic Effect Analyses of a Recombinant Thermostable Newcastle Disease Virus Expressing Cecropin AD

Veterinary Microbiology (2025)

Lun Yao Xiangfei Ren Ping Zhou Junjie Yang Chi Zeng

Newcastle Disease

Cecropin

10.1016/j.vetmic.2025.110397

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Citations (0)

BRD4 Induces Esophageal Squamous Cell Carcinoma Progression via the Wnt/β-catenin Pathway

Biochemical Genetics (2025)

Haiyu Niu Hanwen Wei Xiaochun Zhou Yating Liu Luxi Yang

BRD4, part of the bromodomain and extra terminal domain (BET) protein family, plays a pivotal role in gene transcription, DNA replication, and repair via transcription regulators. Despite its established involvement in various human diseases, its function in esophageal squamous cell carcinoma (ESCC) has not been fully explored. Our research investigated the association of BRD4 in ESCC and its underlying molecular mechanisms. The findings revealed that BRD4 knockdown notably diminished the cells' proliferation, migration, invasion capabilities and induced apoptosis and cell cycle arrest. Conversely, overexpression of BRD4 can reverse these phenotypes. Pearson correlation and enrichment analyses indicated that BRD4 expression was associated with the cell cycle and Wnt/β-catenin signaling pathway. Further validation confirmed that reduced BRD4 expression downregulates Cyclin D1 and c-Myc, and suppresses epithelial-to-mesenchymal transition (EMT) and Wnt/β-catenin signaling pathway. Furthermore, rescue experiments showed that overexpressing c-Myc significantly mitigated the inhibitory impact of BRD4. Moreover, by employing single-cell transcriptome sequencing, we explored the impact of the tumor microenvironment on BRD4 overexpression in ESCC cells. These insights confirmed BRD4's potential as a therapeutic target, suggesting that modulating its expression could yield promising strategies for ESCC treatment.

Beta-catenin

Human genetics

10.1007/s10528-025-11043-0

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MOESM1 of Molecular epidemiology, virulence determinants and antimicrobial resistance of Campylobacter spreading in retail chicken meat in Central China

Figshare (2016)

Téngfēi Zhāng Qingping Luo Yiluo Chen Tingting Li Guoyuan Wen

Additional file 1. Table S1. The results of the durg resistance testing of each strain.

Molecular Epidemiology

10.6084/m9.figshare.c.3614897_d1

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Salmonella Phage vB_SpuM_X5: A Novel Approach to Reducing Salmonella Biofilms with Implications for Food Safety

Microorganisms (2024)

Xinxin Jin Xiuxiu Sun Lu Qin Zui Wang Zhenggang Zhang

Salmonella, a prevalent foodborne pathogen, poses a significant social and economic strain on both food safety and public health. The application of phages in the control of foodborne pathogens represents an emerging research area. In this study, Salmonella pullorum phage vB_SpuM_X5 (phage X5) was isolated from chicken farm sewage samples. The results revealed that phage X5 is a novel Myoviridae phage. Phage X5 has adequate temperature tolerance (28 °C–60 °C), pH stability (4–12), and a broad host range of Salmonella bacteria (87.50% of tested strains). The addition of phage X5 (MOI of 100 and 1000) to milk inoculated with Salmonella reduced the number of Salmonella by 0.72 to 0.93 log10 CFU/mL and 0.66 to 1.06 log10 CFU/mL at 4 °C and 25 °C, respectively. The addition of phage X5 (MOI of 100 and 1000) to chicken breast inoculated with Salmonella reduced bacterial numbers by 1.13 to 2.42 log10 CFU/mL and 0.81 to 1.25 log10 CFU/mL at 4 °C and 25 °C, respectively. Phage X5 has bactericidal activity against Salmonella and can be used as a potential biological bacteriostatic agent to remove mature biofilms of Salmonella or for the prevention and control of Salmonella.

10.3390/microorganisms12122400

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Listeria monocytogenes’in Patojenitesinde Histidin Kinaz Geni yycG’nin Rolü

Kafkas Universitesi Veteriner Fakultesi Dergisi (2020)

Xiaowei Fang Wei Hu Yu Zhang Chen Wang Qingping Luo

As an opportunistic foodborne pathogen, Listeria monocytogenes could successfully switch self-characteristics in response to environmental conditions.In L. monocytogenes, sixteen two-component systems (TCSs) have been found to contribute the bacteria to sensing and adapting to various conditions.Our previous genomic study showed that mutation was observed in the histidine kinase gene yycG between Lm850658 and M7 among the sixteen TCSs.The YycFG TCS was found playing important roles in many other Gram-positive bacteria.While the roles of YycFG TCS in L. monocytogenes remain poorly known.In this study, we aimed to determine whether yycG play roles in pathogenicity of L. monocytogenes.We created a histidine kinase gene yycG deletion strain (ΔyycG) based on the wild type strain Lm850658 and a complemental strain (CΔyycG).We found the yycG deletion significantly impaired the growth ability and mobility, but enhanced the hemolysis ability in vitro.In addition, infection assays on cell and mice model showed that ΔyycG exhibited significantly defected in infection ability and virulence.All these phenotypes of the ΔyycG could be reversed largely to the levels of the wild type strain Lm850658 by gene complementation.Cell wall-associated and secreted protein analysis showed that the secreted content of ΔyycG was significantly increased.And western blotting revealed that Internalin protein B (InlB) and Listerolysin O (LLO) was markedly increased in the secreted fractions of ΔyycG, which might be responsible for decreased adhesion and invasion ability and increased hemolytic activity, respectively.Overall, we found the histidine kinase yycG played important roles in pathogenicity of L. monocytogenes for the first time.Further investigation is needed to explore how the YycFG TCS modulates the growth, mobility, cell surface proteins translocation and virulence.

10.9775/kvfd.2020.24179

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C-terminal truncation of the hemagglutinin-neuraminidase (HN) protein enhances the virulence and immunogenicity of Newcastle disease virus (NDV) vaccine strain V4

Archives of Virology (2023)

Xiangfei Ren Zhe Zeng Yu Shang Lun Yao Li Li

Newcastle Disease

10.1007/s00705-023-05832-4

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Citations (2)

Characterization of a Recombinant Thermostable Newcastle Disease Virus (NDV) Expressing Glycoprotein gB of Infectious Laryngotracheitis Virus (ILTV) Protects Chickens against ILTV Challenge

Viruses (2023)

Zhe Zeng Yan He Zichen Wang Lun Yao Li Li

Infectious laryngotracheitis (ILT) and Newcastle disease (ND) are two important avian diseases that have caused huge economic losses to the poultry industry worldwide. Newcastle disease virus (NDV) has been used as a vector in the development of vaccines and gene delivery. In the present study, we generated a thermostable recombinant NDV (rNDV) expressing the glycoprotein gB (gB) of infectious laryngotracheitis virus (ITLV) based on the full-length cDNA clone of the thermostable TS09-C strain. This thermostable rNDV, named rTS-gB, displayed similar thermostability, growth kinetics, and pathogenicity compared with the parental TS09-C virus. The immunization data showed that rTS-gB induced effective ILTV- and NDV-specific antibody responses and conferred immunization protection against ILTV challenge in chickens. The efficacy of rTS-gB in alleviating clinical signs was similar to that of the commercial attenuated ILTV K317 strain. Furthermore, rTS-gB could significantly reduce viral shedding in cloacal and tracheal samples. Our study suggested that the rNDV strain rTS-gB is a thermostable, safe, and highly efficient vaccine candidate against ILT and ND.

Newcastle Disease

Viral Shedding

Attenuated vaccine

Mononegavirales

Recombinant virus

10.3390/v15020500

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Citations (7)