The main nutrition components and their content in pickled vegetable and in the Pickles were determined.The result showed that the contents of ash,crude protein,crude fat, crude cellulose and water in vegetable and pickled cut cabbage were respectively 4.61 %,15.95%, 10.28%,2.19 %, 23.29% and 3.11 ,26.49 %,15.19%,2.42%,21.05%.Content of amino acids in pickled vegetable and pickled cut cabbage were 74.5045mg/g,66.4876mg/g, respectively.The essential amino acids in the pickles determined were 37.4639 % and 43.6761%,respeetioely.
To establish a method to amplify the entire fragment of genes VP6,VP7,VP4 and NSP4 from group C rotavirus.In order to define the phylogenetic relations among the field strain Wu-82 and other viral strains,4362 stool specimens from patients with diarrhea in all ages were collected during two periods of time,from Dec 2000 to Apr 2003 and from Nov 2003 to Feb 2007.The presence of rotaviruses in stool specimens was determined by detection of the dsRNA fragment of rotavirus by polyacrylamide gel electrophoresis,and the entire fragments of VP7,VP6,VP4 and nsp4 genes were amplified by RT-PCR with 9 pairs of specific primers.Nucleic acid sequence data and hydrophilicity analysis of the deduced amino acids were analyzed using the Gene Works software package and the MEGA 4 program.It was demonstrated that the VP6 and VP7 genes from Wu-82 strain were quite conserved with few mutations,and the phylogenetic relation of the VP6 gene from Wu-82 strain was closer to the Nigerian strain Jajeri and Spanish strain BCN6,whereas the VP7 gene of this strain was closer to Japanese strain E-93 and Columbia strain Javeriana.The sequence of the deduced amino acids of VP6 and VP7 of Wu-82 strain was distinct from that of the 208 strain isolated in 1995 at Wuhan area.Most of the different amino acids between Wu-82 and 208 strains was found to be located in the hydrophilicity regions.The homologies of nucleotide sequences of VP6 and VP7 genes with other group C rotaviruses were 98.45-97.12% and 99.34-94.83% respectively.From the observation of the present study,it is evident that the group C rotavirus genes are very conservative with slow process of evolution,but diversity of epitopes exists in Wu-82 and 208 strains.
[Objective]To select the optimal extraction technology of ursolic acid in Ligustrum lucidum Ait. . [Method]Taking 5% vanillin and perchloric acid as the color reagents,ursolic acid was extracted from L. lucidum by cellulose method. The optimal color condition was determined by orthogonal test; and the content of ursolic acid was determined at 550 nm. [Conclusion]The optimal color condition was 0. 3 ml 5% vanillin,1. 0 ml perchloric acid under 60 ℃. Under this condition,the extraction rate of ursolic acid in L. vicaryi flower was 2. 037%. [Conclusion] Results of orthogonal test showed that the content of ursolic acid was the highest in the fruits of L. vicaryi.
In order to study different drought resistance of root and leaf in maize,water retention ability of leaf,leaf sheath and root at jointing stage of maize(Zea mays L.) two varieties of Yedan 13 and Danyu 13 were examined in field trails.Leaf and sheath of the lower sixth leaf,seminal root and adventitious nodal roots from first layer to fifth layer were used as the test materials.Root was sampled by mining method.The results showed that the water retention ability of different organs were different at jointing stage.From strong to low water retention ability was leaf sheath,root and leaf.To compare water retention ability of different parts of root,from strong to low were base part,middle part and root tip;while to compare that of different root types,water retention ability of seminal root was the weakest.The water retention ability of base part of adventitious nodal roots,from strong to low was the fifth layer,the fourth layer,the third layer,the second layer and the first layer.Water retention ability of middle part and root tip among adventitious nodal roots from first layer to fifth layer showed different.
The primary roots and the first to ninth layers of secondary roots of two maize genotypes of Yedan-13(drought tolerance) and Danyu-13(non-drought tolerance) with different tolerance to drought in the field condition were investigated to study their content of iron,manganese,copper,zinc and sodium by ICP.The results showed that the content of copper of Yedan-13 was higher than that of Danyu-13 at extremely significant difference level(P0.01).And the content of manganese and zinc of Yedan-13 was also significantly higher than that of Danyu-13(P0.05).However,there were no significant differences in the content of iron and sodium of root between the two genotypes (P0.05).
A Preliminary study on the selection of optimum mother culture media for Grifola umbellata was conducted.The experiment results showed that the optimum medium was Potato 100g, Sucrose 10g, KH2PO4 1g, MgSO4 0.25g, VB1 5mg and Agar 9g.
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