There are several testing methods for the detection of Australia antigen. Among them an antibody sensitized latex particle agglutination assay (LAT) for the detection is technologically simple, sensitive, and rapid. So the detection of the antigen in the 896 specimens from hepatic diseases was carried out by LAT. IEOP. and SRID and the detection of rheumatoid factor in the LAT positives was also made by the modified method of Heller et al. The results of testing the 896 specimens from blood donors are positive in 0.8%. negative in 4.1%. false positive in 5.1%. and false negative in 0.1%. And also. it is suggested that false positive to LAT is partially due to rheumatoid factor. since 7 specimens of 9 positives for rheumatoid factor were to LAT (a rate of 67.8%). and partially due to the freshness of blood, since of 59 storage specimens at 4°C for a week there were different reactions in 21 specimens between storage specimens and fresh specimens. Two false negatives in the 61 specimens from hepatic diseases showed positive reactions in diluted specimens for LAT. The cause of false negative reactions to LAT is unclear but it may partly be due to the rheumatoid factor and other factors. These results led us to the conclusions that the LAT requires further development and that the LAT appears to be useful as a preliminary screening for detection of the antigen.
A simple, rapid method is described for separating the serum lipoproteins into clear, discrete and reproducible bands by polyacrylamide disc gel electro phoresis. The sample is prestained with Sudan Black B in a sample gel and resolved by electrophoresis in a discontinuous pH system consisting of a sample gel, concentrating gel and separating gel. 30 young healthy males and 20 young healthy females were examined with the method. The examination of 22 young people of them showed trace amount of chylomicrons. No significant sex difference could be demonstrated in the plasma concentrations of chylomicrons, {3. and pre· f3. and a·lipoproteins as a whole.
By reviewing the outcome, we studied the propriety of our principles for the treatment of type A acute aortic dissection in 45 patients, encountered during the 10-year period between 1981 and 1990. We conducted a comparative study of patients with a thrombosed false lumen (type T) and a patent false lumen (type P) to examine the effect of an acutely thrombosed false lumen on the prognosis of this disease. For the 25 patients treated in the first 6 years (1981-1986), operation was performed as soon as exact diagnosis was made, regardless of the presence of complications and the type or severity of the disease. Early death occurred in 9/20 operated cases and in 4/5 unoperated cases, so 13/25 patients died for a 52.0% mortality rate. For the 20 patients who received treatment in the latter period (1987-1990), we gave priority to conservative treatment for type T cases that were free from complications, and adopted a treatment method attaching greater importance to the resection of intimal tears. As a results, early deaths were observed in only 4 type P patients (20.0%) who underwent operation, a significant better result (p < 0.05). For patients in whom we were able to excise the intimal tear (30.0%, early mortality rate), the results were better than in those in whom the intimal tear were left alone (53.8%). The results were particularly good in type T patients (25.0% of them underwent intimal tear resection and 71.4% underwent no operation for the intimal tear).(ABSTRACT TRUNCATED AT 250 WORDS)
A 71-year-old woman was found to have an abnormal shadow on a chest X-ray. Fifteen years earlier she had undergone a subtotal thyroidectomy for thyroid cancer without any lymph node metastasis. Chest computed tomography (CT) revealed a mediastinal tumor with full of blood stream. Since the positron emission tomography (PET) disclosed an increased uptake of fluoro-2-deoxy-D-glucose (FDG) in the tumor, a malignant lymphatic tumor was therefore suspected. An immunohistological examination of biopsy specimens taken by thoracoscopic procedure demonstrated tumor to be lymph node metastasis of the previous thyroid cancer. After a tumor resection by means of a thoracotomy and total thyroidectomy, the patient was scheduled to receive radioiodine therapy. The previously reported cases are also herein reviewed.
A 71-year-old man with congestive heart failure due to acute myocardial infarction was referred to our hospital. He was under the support of mechanical ventilation and the intraaortic balloon pumping (IABP) and coronary angiogram revealed the thromboembolism of the obtuse marginal artery. We completed the revascularization by the direct percutaneous coronary intervention. However, grade II mitral valve regurgitation and heart failure were worsening. Mitral valvuloplasty and the modified maze procedure through the partial lower sternotomy were performed. He is still in good condition 4 years later. Ischemic mitral valve regurgitation due to the coronary thromboembolism is very rare. Careful follow-up on the grade of ischemic mitral valve regurgitation is necessary even after the early coronary recanalization. The surgical approach of the partial sternotomy should be used in such a case of acute mitral valve regurgitation.
