Shouyi Gao

Iwate University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Biao Kan

National Institute for Communicable Disease Control and Prevention

Yanqing Liu

Columbia University Irving Medical Center

Guoming Qi

National Institute for Communicable Disease Control and Prevention

Meiying Yan

National Institute for Communicable Disease Control and Prevention

Duochun Wang

National Institute for Communicable Disease Control and Prevention

Weili Liang

Hebei Agricultural University

Guangwen Liu

Peking University

Baowei Diao

National Institute for Communicable Disease Control and Prevention

Lijuan Zhang

Tennessee State University

Yongyu Rui

Southern Medical University

Cooperative Institutions

National Institute for Communicable Disease Control and Prevention

Chinese Center For Disease Control and Prevention

Center for Disease Control

State Key Laboratory of Infectious Disease Prevention and Control

Chinese Academy of Medical Sciences & Peking Union Medical College

Chinese Academy of Sciences

Capital Medical University

Sun Yat-sen University

Ministry of Agriculture and Rural Affairs

Zhejiang University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

[Screening for the peptides blocking cholera phage VP1 transfection to host cell by phage random amino acid peptide library].

ACTA MICROBIOLOGICA SINICA (2003)

Duochun Wang Biao Kan Shouyi Gao Yanqing Liu

Use Vibrio cholerae El Tor Typing Phage VP1 as ligand to screen phage-display random 7 amino acid peptide library, ELISA and inactivation experiment were used to identify positive clone. The ratio of output to input was increased after three rounds of screening. Pseudo-positive was decreased stepwise. It indicated the efficient enrichment. After three rounds of screening, 312 out of 360 phage clones were positive in ELISA, 1 clone shows blocking VP1 adsorbs to Vibrio cholerae by inactivation experiment. Sequencing result indicate that amino acid sequences are p245: LeuGlnGlnLysHisLeuLeu; p40, p55: GlnLeuIleMetIleArgHis; p69, p274 IleThrProArgAsnArgSer. Getting some peptides can mimick the VP1 receptor epitopes, one peptide can block VP1 transfection to host cell, it was a clue to study receptor's structure and phage infectious mechanism to host cell.

clone (Java method)

Source

Cite

Citations (0)

Construction and Characterization of a <I>thyA</I> Mutant Derived From Cholera Vaccine Candidate IEM101

Molecular Biotechnology (2005)

Fenggang Yu Guoming Qi Yanqing Liu Shouyi Gao Biao Kan

Cholera

10.1385/mb:29:3:191

Cite

Citations (4)

[Study on infection of different strains of Vibro cholerae O1 by El Tor CTXPhi].

PubMed (2005)

Guangwen Liu Meiying Yan Guoming Qi Shouyi Gao Biao Kan

To study the horizontal transfer efficiencies of filamentous bacteriophage CTXPhi in different V. cholera O1 strains and the phage immunities of these strains. The infectious El Tor CTXPhi particles genetic marked by chloramphenicol resistance gene were used to infect four different V. cholerae O1 strains in vivo and in vitro. Selected the infected clones based on its character of chloramphenicol resistance and identified and judged the exist form of CTXPhi genome through Southern bolt and other hybridization methods. Calculated the infection rates of different strains and compared each other. Then we analyzed the mechanism of infection and phage immunity. The infection rate of classic strain 1119 with the genetic marked CTX(ET)Phi in vivo is much higher than that in vitro. In vitro experiment, the rate of 1119 is higher than other three El Tor strains. And in El Tor strains, the infection frequency of IEM101 that had no rstR gene is 100 to 1000 times higher than other two strains containing rstR. Classical biotype strain is more susceptible to CTX(ET)Phi particles than El Tor strains. Expression of TCP and the phage immunity mediated by rstR gene affect the horizontal transfection of CTXPhi in V. cholerae strains.

El Tor

Strain (injury)

Cholera

Source

Cite

Citations (1)

Proteins involved in difference of sorbitol fermentation rates of the toxigenic and nontoxigenic Vibrio choleraeEl Tor strains revealed by comparative proteome analysis

BMC Microbiology (2009)

Ruibai Wang Hongzhi Zhang Haiyan Qiu Shouyi Gao Biao Kan

Abstract Background The nontoxigenic V. cholerae El Tor strains ferment sorbitol faster than the toxigenic strains, hence fast-fermenting and slow-fermenting strains are defined by sorbitol fermentation test. This test has been used for more than 40 years in cholera surveillance and strain analysis in China. Understanding of the mechanisms of sorbitol metabolism of the toxigenic and nontoxigenic strains may help to explore the genome and metabolism divergence in these strains. Here we used comparative proteomic analysis to find the proteins which may be involved in such metabolic difference. Results We found the production of formate and lactic acid in the sorbitol fermentation medium of the nontoxigenic strain was earlier than of the toxigenic strain. We compared the protein expression profiles of the toxigenic strain N16961 and nontoxigenic strain JS32 cultured in sorbitol fermentation medium, by using fructose fermentation medium as the control. Seventy-three differential protein spots were found and further identified by MALDI-MS. The difference of product of fructose-specific IIA/FPR component gene and mannitol-1-P dehydrogenase, may be involved in the difference of sorbitol transportation and dehydrogenation in the sorbitol fast- and slow-fermenting strains. The difference of the relative transcription levels of pyruvate formate-lyase to pyruvate dehydrogenase between the toxigenic and nontoxigenic strains may be also responsible for the time and ability difference of formate production between these strains. Conclusion Multiple factors involved in different metabolism steps may affect the sorbitol fermentation in the toxigenic and nontoxigenic strains of V. cholerae El Tor.

Sorbitol dehydrogenase

10.1186/1471-2180-9-135

Cite

Citations (7)

Complete genome sequence and analysis of {\sl Vibrio cholerae} phage VP2

Bingdu xuebao (2005)

Duochun Wang Min Wang Yanping Li Hui Dong Zhonghua Liu

The results showed that VP2 belongs to Podoviridae, the circular double-stranded DNA (ds DNA) VP2 genome consists of 29853 bp, with overcall G+C content of 50.56%, which was higher than the G+C content of N16961, a sequenced strain of Vibrio cholerae. Putative forty-five open reading frames (ORF) were annotated, homology analysis were used to assign possible functions for the genes, including, DNA replication, synthesis of capsid protein, DNA packaged genes, and infection-associated genes. The result of phylogenetic tree of DNA pol showed VP2 was clustered together with phage Cp-1 and GA-1. The evolutive analysis above suggests VP2 belongs to phi29-like phage.

Podoviridae

Source

Cite

Citations (0)

Sequencing and analysis of Vibrio cholerae typing phage VP3 genome

Yingwei Zhao Duochun Wang Min Wang Yanping Li Biao Kan

DNA sequence and the genome of phage VP3(a typing phage of V. cholera) were analyzed. A random library of VP3 DNA was constructed by shot-gun library method. The VP3 genome sequence was assembled with contigs sequences, the gaps between different contigs were filled with sequencing data from primer walking. ORFs were predicted; Phylogeny of DNA polymerase sequences was analyzed to determine the class of VP3; The activity of putative promoter genes were analyzed using %lacZ% report system. VP3 genome is a 39504bp of circular double-stranded DNA. Twenty-seven out of forty-nine putative ORFs were annotated; twenty gene products were homologous with T7-like phages, including DNAP, DNA replicative protein, capsid, tail tubular, tail fiber protein, and DNA packaged protein. The activity of the putative promoter regions was confirmed through cloning those regions to LacZ-fuse plasmid pRS1274 and analysis of the expression of beta galactosidase. The complete genomic sequence of VP3 and phylogenetic tree analysis suggests VP3 is a member of T7 phage family.

ORFS

Source

Cite

Citations (1)

Resistance of the cholera vaccine candidate IEM108 against CTXΦ infection

Vaccine (2005)

Guangwen Liu Meiying Yan Weili Liang Guoming Qi Yanqing Liu

Cholera toxin

El Tor

Cholera

Cholera vaccine

Lysogenic cycle

10.1016/j.vaccine.2005.09.059

Cite

Citations (12)

[A study on genetic polymorphism of rRNA gene pattern of Vibrio cholerae O139 in China].

PubMed (2002)

Mei Qu Biao Kan Guoming Qi Yanqing Liu Shouyi Gao

To investigated the genetic polymorphism of the isolated strains using ribotyping method.One hundred twenty-two strains of V. cholerae O139 isolated from different areas of China from 1993 to 1999 were selected and characterized with ribotyping, including 16s rDNA and 23s rDNA probes.One hundred twenty-two strains were differentiated into 10 different ribotypes (RT1-RT10) on the basis of rRNA gene probes hybridization (with Bgl I digestion), which consisted of 7 - 9 bands between 12 and 1.5 kb in size. RT1 and RT3, as two predominant ribotypes, comprised most number of the strains which spread to the extensive range. Nine strains, which are negative to ctxAB, zot and RS individually, belong to 4 special ribotypes. The dendrogram revealing genetic relationship among different clones of V. cholerae O139 showed that the clones belonging to RT1 and RT2 had genetic similarity on high degree, although they were isolated from different regions. The two predominant ribotypes (RT1 and RT3) were distant in genetic relationship.Results showed the clonal diversity and the wide area distribution of V. cholerae O139 strains in China, suggesting the multiple origins of O139 epidemics.

Ribotyping

Dendrogram

Source

Cite

Citations (0)

Molecular characterization of a novel adult diarrhoea rotavirus strain J19 isolated in China and its significance for the evolution and origin of group B rotaviruses

Journal of General Virology (2008)

Shengjun Jiang Shaozhong Ji Qing Tang Xiaoying Cui Hongyang Yang

The complete genome of a novel adult diarrhoea rotavirus strain J19 was cloned and sequenced using an improved single-primer sequence-independent method. The complete genome is 17,961 bp and is AU-rich (66.49 %). Northern blot analysis and genomic sequence analysis indicated that segments 1-11 encode 11 viral proteins, respectively. Protein alignments with the corresponding proteins of J19 with B219, and groups A, B and C rotaviruses, produced higher per cent sequence identities to B219. Among groups A, B and C rotaviruses, 10 proteins from group B rotaviruses exhibited slightly higher amino acid sequence identity to the J19 proteins, but proteins of J19 showed low amino acid sequence identity with groups A and C rotaviruses. Construction of unrooted phylogenetic trees using a set of known proteins and representatives of three known rotavirus groups revealed that six structural proteins were positioned close to B219 and the basal nodes of groups A, B and C lineages, although with a preferred association with group B lineages. Phylogenetic analysis of the five non-structural proteins showed a similar trend. The results of the serological analysis, protein sequence analysis and phylogenetic analysis suggested that J19 would be a novel rotavirus strain with great significance to the evolution and origin of group B rotaviruses.

Group A

10.1099/vir.0.2008/001933-0

Cite

Citations (42)

Pleiotropic effects of the twin-arginine translocation system on biofilm formation, colonization, and virulence in Vibrio cholerae

BMC Microbiology (2009)

Lijuan Zhang Zhaoqin Zhu Huaiqi Jing Jingyun Zhang Yanwen Xiong

The Twin-arginine translocation (Tat) system serves to translocate folded proteins, including periplasmic enzymes that bind redox cofactors in bacteria. The Tat system is also a determinant of virulence in some pathogenic bacteria, related to pleiotropic effects including growth, motility, and the secretion of some virulent factors. The contribution of the Tat pathway to Vibrio cholerae has not been explored. Here we investigated the functionality of the Tat system in V. cholerae, the etiologic agent of cholera.In V. cholerae, the tatABC genes function in the translocation of TMAO reductase. Deletion of the tatABC genes led to a significant decrease in biofilm formation, the ability to attach to HT-29 cells, and the ability to colonize suckling mouse intestines. In addition, we observed a reduction in the output of cholera toxin, which may be due to the decreased transcription level of the toxin gene in tatABC mutants, suggesting an indirect effect of the mutation on toxin production. No obvious differences in flagellum biosynthesis and motility were found between the tatABC mutant and the parental strain, showing a variable effect of Tat in different bacteria.The Tat system contributes to the survival of V. cholerae in the environment and in vivo, and it may be associated with its virulence.

Parasitology

10.1186/1471-2180-9-114

Cite

Citations (37)