Santanu K. Ghosh

Indian Institute of Technology Bombay

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Syed Meraj Azhar Rizvi

University of California, Irvine

Hemant Kumar Prajapati

National Institutes of Health

Makkuni Jayaram

The University of Texas at Austin

Masaki Ito

The University of Tokyo

Goutam Chowdhury

National Institute of Cholera and Enteric Diseases

Sanjeeva Srivastava

Indian Institute of Technology Bombay

Hong Cui

University of Toronto

Ishan Rathore

National Cancer Institute

Sujata Hajra

The University of Texas at Austin

Gururaja Perumal Pazhani

SRM Institute of Science and Technology

Cooperative Institutions

Indian Institute of Technology Bombay

National Institute of Cholera and Enteric Diseases

Translational Health Science and Technology Institute

National Institute of Pharmaceutical Education and Research

The University of Texas at Austin

National Institutes of Health

Indian Council of Medical Research

Sapporo Medical University

University of Southern California

Nagoya University

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The selfish yeast plasmid uses the nuclear motor Kip1p but not Cin8p for its localization and equal segregation

The Journal of Cell Biology (2009)

Hong Cui Santanu K. Ghosh Makkuni Jayaram

The 2 micron plasmid of Saccharomyces cerevisiae uses the Kip1 motor, but not the functionally redundant Cin8 motor, for its precise nuclear localization and equal segregation. The timing and lifetime of Kip1p association with the plasmid partitioning locus STB are consistent with Kip1p being an authentic component of the plasmid partitioning complex. Kip1-STB association is not blocked by disassembling the mitotic spindle. Lack of Kip1p disrupts recruitment of the cohesin complex at STB and cohesion of replicated plasmid molecules. Colocalization of a 2 micron reporter plasmid with Kip1p in close proximity to the spindle pole body is reminiscent of that of a CEN reporter plasmid. Absence of Kip1p displaces the plasmid from this nuclear address, where it has the potential to tether to a chromosome or poach chromosome segregation factors. Exploiting Kip1p, which is subsidiary to Cin8p for chromosome segregation, to direct itself to a "partitioning center" represents yet another facet of the benign parasitism of the yeast plasmid.

Spindle pole body

Colocalization

10.1083/jcb.200810130

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Citations (43)

Comparative proteomics of mitosis and meiosis in Saccharomyces cerevisiae

Journal of Proteomics (2014)

Ravinder Kumar Snigdha Dhali Srikanth Rapole Santanu K. Ghosh Sanjeeva Srivastava

10.1016/j.jprot.2014.06.006

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Faithful segregation of the multicopy yeast plasmid through cohesin-mediated recognition of sisters

Proceedings of the National Academy of Sciences (2007)

Santanu K. Ghosh Sujata Hajra Makkuni Jayaram

The 2-microm yeast plasmid, a benign high-copy nuclear parasite, propagates itself with nearly the same fidelity as the chromosomes of its host. Equal plasmid segregation is absolutely dependent on the cohesin complex assembled at the plasmid partitioning locus STB. However, the mechanism of cohesin action in the context of multiple plasmid copies, resident within two separate clusters after DNA replication, is unknown. By using "single-copy" derivatives of the 2-microm plasmid, we demonstrate that recruitment of cohesin at STB during S phase indeed translates into cohesion between plasmid molecules. Through binary fluorescence tagging, we reveal that segregation of replicated plasmids occurs in a sister-to-sister fashion. Thus, cohesin serves the same fundamental purpose in plasmid and chromosome segregation.

10.1073/pnas.0702996104

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Isolation and Characterization of Pandemic and Nonpandemic Strains ofVibrio parahaemolyticusfrom an Outbreak of Diarrhea in North 24 Parganas, West Bengal, India

Foodborne Pathogens and Disease (2013)

Goutam Chowdhury Santanu K. Ghosh Gururaja Perumal Pazhani Bimal Kanti Paul Dipankar Maji

Strains of the enteric pathogen Vibrio parahaemolyticus harboring the thermostable hemolysin (TDH) encoding gene tdh is known to cause epidemic and pandemic diarrhea. In industrialized countries, this pathogen causes sporadic or outbreaks of diarrheal illness associated with consumption of raw or improperly cooked seafood. This report describes a foodborne outbreak of gastroenteritis caused by V. parahaemolyticus in June 2011 following consumption of food served at a funeral reception held at Habra, North 24 Parganas, West Bengal, India. About 650 people attended the function, of whom 44 had acute watery diarrhea with other clinical symptoms; 35 of them were admitted to the District Hospital for the rehydration treatment. Stool specimens collected from three hospitalized cases were positive for V. parahaemolyticus, of which two strains were identified as an O4:K8 serovar and one was identified as O3:K6 serovar. The O3:K6 strain also possessed the pandemic group-specific toxRS gene target (GS), whereas the O4:K8 strains were negative. All strains were polymerase chain reaction–positive for tdh but were polymerase chain reaction–negative for trh. All of the strains were resistant to ampicillin but were pansensitive to other antimicrobials tested. Pulsed-field gel electrophoresis (PFGE) analysis using NotI showed that the O3:K6 strain was similar to that of a recent clinical strain from Kolkata, but had diverged from other strains during previous years. In contrast, PFGE analysis showed that the O4:K8 strains were closely related but differed from the Kolkata strain.

Pandemic

10.1089/fpd.2012.1340

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Citations (16)

Modified Kligler's Iron Agar Medium for the Isolation of Intestinal Pathogens.

PubMed (1945)

G Panja Santanu K. Ghosh

Isolation

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Microtubule‐associated proteins, Bik1 and Bim1, are required for faithful partitioning of the endogenous 2 micron plasmids in budding yeast

Molecular Microbiology (2016)

Hemant Kumar Prajapati Syed Meraj Azhar Rizvi Ishan Rathore Santanu K. Ghosh

The 2 μ plasmid of budding yeast shows high mitotic stability similar to that of chromosomes by using its self-encoded systems, namely partitioning and amplification. The partitioning system consists of the plasmid-borne proteins Rep1, Rep2 and a cis-acting locus STB that, along with several host factors, ensures efficient segregation of the plasmid. The plasmids show high stability as they presumably co-segregate with chromosomes through utilization of various host factors. To acquire these host factors, the plasmids are thought to localize to a certain sub-nuclear locale probably assisted by the motor protein, Kip1 and microtubules. Here, we show that the microtubule-associated proteins Bik1 and Bim1 are also important host factors in this process, perhaps by acting as an adapter between the plasmid and the motor and thus helping to anchor the plasmid to microtubules. Abrogation of Kip1 recruitment at STB in the absence of Bik1 argues for its function at STB upstream of Kip1. Consistent with this, both Bik1 and Bim1 associate with plasmids without any assistance from the Rep proteins. As observed earlier with other host factors, lack of Bik1 or Bim1 also causes a cohesion defect between sister plasmids leading to plasmid missegregation.

Budding yeast

10.1111/mmi.13608

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The 2 micron plasmid: a selfish genetic element with an optimized survival strategy within Saccharomyces cerevisiae

Current Genetics (2017)

Syed Meraj Azhar Rizvi Hemant Kumar Prajapati Santanu K. Ghosh

T-DNA Binary system

10.1007/s00294-017-0719-2

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Citations (38)

Isolation of acdc28 mutation that abrogates the dependence of S phase on completion of M phase of the budding yeast cell cycle

Journal of Genetics (2000)

Santanu K. Ghosh Pratima Sinha

Budding

S phase

10.1007/bf02715856

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The 2-μm plasmid encoded protein Raf1 regulates both stability and copy number of the plasmid by blocking the formation of the Rep1–Rep2 repressor complex

Nucleic Acids Research (2017)

Syed Meraj Azhar Rizvi Hemant Kumar Prajapati Purba Nag Santanu K. Ghosh

The 2-μm plasmid of the budding yeast Saccharomyces cerevisiae achieves a high chromosome-like stability with the help of four plasmid-encoded (Rep1, Rep2, Raf1 and Flp) and several host-encoded proteins. Rep1 and Rep2 and the DNA locus STB form the partitioning system ensuring equal segregation of the plasmid. The Flp recombinase and its target sites FRTs form the amplification system which is responsible for the steady state plasmid copy number. In this work we show that the absence of Raf1 can affect both the plasmid stability and the steady sate copy number. We also show that the Rep proteins do bind to the promoter regions of the 2-μm encoded genes, as predicted by earlier models and Raf1 indeed blocks the formation of the Rep1–Rep2 repressor complex not by blocking the transcription of the REP1 and REP2 genes but by physically associating with the Rep proteins and negating their interactions. This explains the role of Raf1 in both the partitioning and the amplification systems as the Rep1–Rep2 complex is believed to modulate both these systems. Based on this study, we have provided, from a systems biology perspective, a model for the mechanism of the 2-μm plasmid maintenance.

10.1093/nar/gkx316

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Citations (14)

Role of Ctf3 and COMA subcomplexes in meiosis: Implication in maintaining Cse4 at the centromere and numeric spindle poles

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research (2015)

Meenakshi Agarwal Gunjan Mehta Santanu K. Ghosh

Meiosis II

10.1016/j.bbamcr.2014.12.032

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Citations (16)