D. Sreenivasulu

Post Graduate Institute of Medical Education and Research

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Ch. Srilatha

Professor Jayashankar Telangana State Agricultural University

P. Amaravathi

Sri Venkateswara Veterinary University

K. Sujatha

Dr. M.G.R. Educational and Research Institute

Tentu Nageswara Rao

Krishna University

V. Ramadevi

Government Kilpauk Medical College

P. Eswara Prasad

P.V. Narsimha Rao Telangana Veterinary University

B. Bhaskar Reddy

Acharya N. G. Ranga Agricultural University

Tentu Nageswara Rao

Krishna University

S. Sivajothi

Sri Venkateswara Veterinary University

Ting Rao

Renmin Hospital of Wuhan University

Cooperative Institutions

Post Graduate Institute of Medical Education and Research

Sri Venkateswara Veterinary University

Institute of Post Graduate Medical Education and Research

All India Institute of Medical Sciences

University of Veterinary Medicine

GITAM University

Acharya N. G. Ranga Agricultural University

Krishna University

All India Institute of Medical Sciences Bhubaneswar

Institute of Medical Sciences

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Detection of bluetongue virus (BTV) in clinical samples by NS3 specific reverse transcription chain reaction (RT-PCR)1

Indian Journal of Comparative Microbiology Immunology and Infectious Diseases (2006)

B. V. Devi D. Sreenivasulu Chetty

NS3 specific RT-PCR for detection of bluetongue virus (BTV) in clinical samples has been standardized using NS3 primers. Trizol® method of RNA extraction was used to isolate RNA from infected cell culture fluids, embryonated chicken eggs and blood samples. Denaturation of dsRNA by heating at 95°C for 5 minutes in the pesence of 5% DMSO and a 35 amplification cycle PCR with annealing temperature of 59°C for 30 seconds at 1.5 mM concentration of MgCl2 and primer extension at 72°C for 1 minute were found optimum to obtain 780 bp PCR product. The RT- PCR thus standardized has detected BTV RNA in blood samples collected between days 5 to 11 post inoculation from experimentally infected sheep. Out of 32 field blood samples collected from bluetongue suspected outbreaks of A.P. during the months of August and September, 2005, ten were found positive for the presence of BTV RNA by NS3 RT-PCR. Further, BTV was isolated from PCR positive blood samples by intravenous inoculation into embryonated chicken eggs and subsequent passages on to BHK21 cell line and identified as BTV by nucleic acid migration pattern in 1% agarose gel electrophoresis. This confirmed the utility of NS3 RT-PCR as a test for rapid and confirmatory diagnosis of bluetongue infection in sheep.

Embryonated

Trizol

Agarose gel electrophoresis

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ESTIMATION OF GRANISETRON HYDROCHLORIDE BY A NEW RP-HPLC METHOD

International Journal of Pharma and Bio Sciences (2012)

Tentu Nageswara Rao D. Sreenivasulu P.U.V. Satish T. Parvathamma

A simple, sensitive and specific reverse phase high performance liquid chromatographic method has been developed for the determination of granisetron hydrochloride in injectable dosage forms. Chromatographic separation was achieved on a peerless basic C18 (250×4.6 mm), 5.0 'm column with a diluted 1.6 ml of ortho phosphoric acid in 800 ml water, added 200ml acetonitrile, added 1.0ml of Hexylamine and mixed. Then adjusted the pH to 7.5 with triethylamine as mobile phase, detection was at 304 nm. Response was a linear function of concentration in the range 5.0.02 mg/L for granisetron hydrochloride. LOD and LOQ for granisetron hydrochloride were found 0.01 mg/L and 0.03 mg/L. Accuracy (recoveries 94.97%) and reproducibility were found to satisfactory.

Triethylamine

Phosphoric acid

Granisetron

Hydrochloride

Chromatographic Separation

Linear range

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EFFECT OF LEAD ACETATE ON SPERM MORPHOLOGY AND TESTIS OF WISTAR ALBINO RATS

The Indian Journal of Animal Reproduction (2015)

K. Sujatha Ch. Srilatha Y. Anjaneyulu Ting Rao D. Sreenivasulu

In the present study different doses of lead acetate was used to know the effect on testis, sperm count, sperm morphology in different experimental groups. 108 male wistar rats were divided into three equal groups (I, II &III) i.e. each group contains 36 rats. Group I kept as control. Group II & III rats were orally administered lead acetate @ 60 mgs & 30mgs /kg. bwt / 3 days in a week for 90 days. Every fortnight from each group rats were sacrificed with anesthetic ether and epididymis collected for sperm count, testes for measurement of testicular weight and pieces of testis fixed in Bovin’s fixative for histopathological and histochemical studies. The study revealed that lead acetate caused a dose dependant reduction in sperm count, testicular weight, and abnormal sperm count was increased significantly when compared to control. Hisopathologically testis shows necrosis of seminiferous tubules. Complete separation and disappearance of seminiferous tubular epithelium. Lead also caused reduction in testicular alkaline phosphatase activity.

Lead acetate

Seminiferous tubule

Fixative

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Study on effect of chitosan on body weight gain and anti-microbial activity in swiss albino mice

Journal of Animal Feed Science and Technology (2016)

D. Rani Prameela D. Sreenivasulu A. Ravi M. Amaravathi W.L.N.V. Varaprasad

10.21088/jafst.2321.1628.4216.1

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Histopathological and immune-histochemical diagnosis of Burkitt Type Lymphoma in a cross bred cow

Journal of Livestock Science (2019)

WLNV Vara Prasad K. Sujatha C. Latha D. Sreenivasulu K. Adilaxmamma

Detailed pathomorphological study was carried out on tumor suspected mesenteric lymphnode from a Jersey cross bred cow.Uniform population of small non cleaved cells with prominent nucleoli were observed in cytological study, histopathology revealed characteristic starry sky appearence or moth eaten appearance of lymph node with diffuse infiltration of neoplastic cells in cortex and medulla.Immunohistochemistry shown positive reaction for common acute lymphocytic leukemia antigen (CD10), terminal deoxynucleotidyl transferase (TdT) and cluster of differentiation 5 (CD5) and negative reaction for cluster of differentiation 3 (CD3), B-cell lymphoma 2 (Bcl2), cluster of differentiation 20 (CD20) and cluster of differentiation 79a (CD79a).These Cytological, histopathological and immunohistochemical findings observed led to final diagnosis of burkitt lymphoma.

Burkitt's lymphoma

10.33259/jlivestsci.2019.81-85

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Characterization of indigenous goatpox virus strains

Indian Journal of Comparative Microbiology Immunology and Infectious Diseases (2009)

K. Lakshmi Kavitha Manogari Chetty D. Sreenivasulu Srilatha

The four strains (TPT, ANT, KDP and CHI) of goatpox virus were characterized. The virus showed loss of infectivity at 55°C for 60 min, 60°C for 30 min, 70°C for 10 min, pH3 and 5 for one hour, chloroform and ether. Under electron microscopy, the viral particles appeared as brick shaped while under immunoelectron microscopy, a clear cut aggregation of viral particles was observed with the specific serum against goatpox virus. Antigenically, the virus revealed three precipitation bands and arcs in AGID and IEP, respectively, whereas molecularly under SDS-PAGE the virus showed 17 co-migrating fragments, of which five were major fragments having molecular weights of 67K, 64K, 47K, 30K and 22K. While with RFLP, the virus strains revealed 95 percent homology by generating 15 bands of size ranging from 31 Kb to 2 Kb.

Immunoelectron microscopy

Infectivity

Homology

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Standardization of reverse transcriptase - polymerase chain reaction for the detection of paraflagellar rod gene of Trypanosoma evansi in cattle

International Journal of Livestock Research (2018)

S. Sivajothi VC Rayulu P. M. Kondaiah D. Sreenivasulu Ch. Srilatha

Trypanosoma evansi

10.5455/ijlr.20171021090945

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A GC-MS method for the determination of Mancozeb and Metiram (as CS2) residues in Aquatic Tox medium.

Scholars Academic Journal of Pharmacy (2013)

Tentu Nageswara Rao D. Sreenivasulu T. B. Patrudu K. M. S. Sreenivas Botsa Parvatamma

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Delivery of livestock extension services - an overview.

G. Triveni D. Sreenivasulu

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Pulmonary and genotoxicity of Bisphenol-A in Wistar albino rats

Current biotica (2012)

P. Amaravathi Ch. Srilatha V. Ramadevi D. Sreenivasulu Preethi Prasad

Bisphenol A (BPA) is one of the common environmental endocrine disruptors with estrogenic properties and is the building block of carbonate plastic and a component of resin coatings. Wide spread use of BPA in consumer products has led to a great public concern since adverse effects of BPA on human and animal reproduction are suspected due to its estrogenic activity. The present experiment was designed to make a systematic study of experimentally induced BPA toxicity in both male and female Wistar albino rats at 500 and 250 mg /kg b.wt. to groups II & V and III & VI, respectively by mixing in sunflower oil for 12 weeks, with the objective of finding out the effect of BPA toxicity on DNA and resultant histopathological changes in lungs and trachea. DNA damage was evident as significant increase in micronuclei in poly chromatophilic erythrocytes of BPA treated groups when compared to control. Microscopically, lungs revealed congestion, fatty infiltration, peribronchial lymphoid aggregates and infiltration of eosinophils, vacuolated alveolar epithelial cells, infiltration of alveolar macrophages, hyperplasia of bronchiolar epithelium and focal alveolar epithelial cell proliferation with papillary projections and desquamated bronchial epithelial cells were found as constant lesion by the end of 12 th week in a dose dependent manner.

Infiltration (HVAC)

Endocrine disruptor

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Citations (6)