Bone marrows from 30 newly diagnosed Ph+chronic myelocytic leukemia (CML) (21 in chronic phase, CML-CP, 9 in accelerate phase, CML-AP) and 8 followed up patients in blast crisis (CML-BC) were tested by DNA strand breaks (%D value), DNA-aneuploidy, flow cytometry-cell surface antigen expression for CD15 and HLA-DR ratio. Our results showed that all these three parameters changed as the disease escalated. A very low value of %D and DNA-aneuploidy occurrences were high risk factors. Cell surface antigen expression CD15 and HLA-DR ratio measurement was simple and reliable. The ratio < 1.0 appeared earlier than morphology clarifying CML-AP and should be followed up regularly.
This study aimed at exploring and contrasting the clinical significances and values of MRI, CT and contrast-enhanced ultrasonography in FIGO staging of cervical carcinoma.The contrast-enhanced ultrasonography, CT and MRI imaging data of 348 patients with cervical carcinoma confirmed by clinical pathology were analyzed retrospectively and contrasted with pathological findings.The total accuracy of MRI in cervical carcinoma staging was 79.89% (278/348), and the diagnostic accuracy of MRI in stage IB, stage II, stage III and stage IV of cervical carcinoma was 74.29% (26/35), 75.74% (153/202), 85.25% (52/61), 94.00% (47/50), respectively. The total accuracy of CT in cervical carcinoma staging was 73.28% (255/348), and the diagnostic accuracy of CT in stage IB, stage II, stage III and stage IV of cervical carcinoma was 60.00% (21/35), 69.80% (141/202), 78.69% (48/61), 94.00% (45/50), respectively. The total accuracy of contrast-enhanced ultrasonography in cervical carcinoma staging was 57.47% (200/348), and the diagnostic accuracy of contrast-enhanced ultrasonography in stage IB, stage II, stage III and stage IV of cervical carcinoma was 37.14% (13/35), 50.99% (103/202), 70.49% (43/61), 82.00% (41/50), respectively. The accuracy of MRI in the diagnosis of stage IB, stage II of cervical carcinoma was higher than that of CT and contrast-enhanced ultrasonography (p<0.05), and the diagnostic accuracy of CT was higher than that of contrast-enhanced ultrasonography (p<0.05). The differences among the three methods were statistically significant.According to the results of pathological sections, there were statistically significant differences among the sensitivity and specificity of MRI, CT and contrast-enhanced ultrasonography in the diagnosis of stage IB and stage II (p<0.05). MRI has high diagnostic values in the differentiation and diagnosis of cervical carcinoma staging.
Pancreatic cancer (PC) is the most malignant tumor among all the tumors in the digestive system. MiR-217 has been reported to take a critical part in various malignant tumors. The aim of this study was to explore the function of MiR-217 in pancreatic cancer and its target genes.Twenty pairs of PC tissues and matched normal adjacent pancreatic tissues were collected. The expression of miR-217 in PC tissues and normal pancreatic tissues was detected by Real-time polymerase chain reaction (PCR). PC cells were transfected with miR-217 mimics, inhibitors and negative control, respectively. Cell Counting Kit-8 (CCK-8) assay was used to detect cell viability. Cell apoptosis was checked via Annexin V-FITC/PI apoptosis kit. The protein expression of E2F3 was detected by Western blot. To detect repression by miR-217, HEK293T cells were co-transfected with the indicated E2F3 3'-UTR luciferase reporter.The expression of miR-217 was reduced in PC tissues comparing to normal pancreatic tissues. Meantime, the in-vitro study revealed that miR-217 suppressed PC cell growth, invasion but promoted apoptosis. Next, we proved that E2F3 was the target of miR-217 on PC cell function.miR-217 suppresses PC cell growth, invasion but promotes apoptosis in vitro through targeting E2F3. The miR-217-E2F3 axis may be used for PC therapy.
Newborn rats were exposed to a single episode of neonatal hypoxia and were studied at intervals from 4 hours to 3 months after the exposure. Endothelial cells showed unusual darkening of the cytoplasm, and protrusion of the soma into the lumen of the vessels. Fibroblasts showed unusually dilated cisternae of rough endoplasmic reticulum. The perivascular cells were also unusual, in that they had an 'activated' appearance as suggested by the presence of large vacuoles and secondary lysosomes, and were often connected to the vessel walls by only a thin foot process. The basal lamina often gave off long extensions into the neuropil or incompletely surrounded the cells of the vessel walls. The astrocytic foot processes were swollen and contained many glycogen granules and loosely packed glial filaments. All the above changes were present even at 3 months posthypoxia. The discontinuous basal lamina could allow substances to pass more easily from the bloodstream into the neuropil. It is also possible, in view of the frequent observations of perivascular cells that were connected to the vessel walls by only a thin foot process, that these extensions could be places where perivascular cells leave the vessels to enter the neuropil. It is postulated that increased movement of 'activated' perivascular cells into the neuropil could result in increased pruning of neuronal processes in the neuropil, after the episode of neonatal hypoxia.
The expression of gamma-glutamyl transpeptidase (GGT) has been associated with the emergence of a functional blood-brain barrier. We have undertaken a precise localisation of this enzyme in the cerebral cortical vessels of different species, by immunocytochemistry using a polyclonal antibody and light and electron microscopy. GGT immunoreaction product was present on the luminal surface of endothelial cells in 1-day-old to 3-month-old rats, whereas in the mouse, monkey and human cortex, this protein was present in astrocytic endfeet around the vessels. No labelling was observed in the other cellular components of the vessel walls, such as pericytes, fibroblasts, smooth muscle cells and perivascular cells. The localisation of GGT in astrocytes in mice, monkeys and humans suggests that these cells could play a role in the detoxication of lipophilic xenobiotic substances that cross the endothelial barrier. In these species, astrocytes can be viewed as a second line of defense against xenobiotics, beyond the capillary endothelium.
This study aimed to determine the effect of Lactuside B isolated from Pterocypsela alata on the expression of AQP4 and TRPM7 mRNAs after cerebral ischemic injury.Brain ischemia injury was established by occluding the MCA (middle cerebral artery) for 2 h, followed by reperfusion in rats. The neurologic deficit scores were used to determine the success of the model. All drugs were intraperitoneally administered once a day (5 ml/kg). Eight animals from each group were investigated for the Na+ level, and the others were examined for AQP4 and TRPM7 mRNA changes.Compared with the model group, the neurologic deficit scores and Na+ levels decreased in the lactuside B groups (p < 0.05 vs. p < 0.01). All lactuside B groups had significantly decreased AQP4 and TRPM7 mRNA expression compared with the model group (p < 0.05 vs. < 0.01). Dose dependence was observed between low and medium doses.Lactuside B protected against cerebral edema and nerve cell damage caused by cerebral ischemic injury by decreasing the expression of AQP4 and TRPM7 mRNAs in the cerebral cortex of rats.
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