Planting date of soybean, Glycine max, influenced winter survival of Pratylenchus brachyurus in microplots at two locations in North Carolina. Delayed planting resulted in a linear decrease (P = 0.05) in the numbers of P. brachyurus at soybean harvest. Effects of planting date on nematode numbers persisted over winter, indicating that survival in the absence of a host is density independent. Compared with winter fallow, winter wheat, Triticum aestivum, reduced winter survival of P. brachyurus. Subsequent soybean yields were suppressed by the overwintering population of this nematode at one location but not at another.
The biology, pathogenicity, and management of Pralylenchus species have been discussed. This paper evaluates current research in order to propose tactics for managing Pratylenchus brachyurus (Godfrey) Filipjev and Schuurmans Stekhoven on soybean, Glycince max (L.) Merr
The effects of Crotalaria juncea , Brassica napus and Tagetes erecta on resistance, allelopathic suppression, and enhancement of nematode antagonists against Rotylenchulus reniformis were examined in a series of greenhouse experiments. Crotalaria juncea and B. napus are poor hosts to R. reniformis as compared to Vigna unguiculata . Tagetes erecta was as good a host for R. reniformis as was Ananas comosus. Crotalaria juncea delayed the development of female nematodes compared to V. unguiculata . Allelopathic effects against R. reniformis were most pronounced in leaf leachate of C. juncea 2 days after incorporation where the viability of the nematode was suppressed to 0.5% as opposed to 60% when the R . reniformis were incubated overnight in leachates of B. napus , T. erecta , A. comosus , sand or distilled water. Amendment with C. juncea was most efficient in enhancing parasitic nematode-trapping fungi, R. reniformis egg-parasitic fungi, vermiform stage parasites, and bacterivorous nematodes compared to B. napus, and T. erecta leaf amendments. Bare soil and 1,3-Dichloropropene treatments suppressed nematode-trapping fungal population densities. Suppression of R. reniformis development on V . unguiculata by these crop amendments was inconclusive due to the short cowpea bioassay period. However, C. juncea amendments enhanced cowpea growth more than did the other soil amendment treatments. Among the crops tested, C. juncea is the most promising cover crop for R. reniformis management.
Population fluctuations of Heterodera glycines differ in fields with high and low initial population densities. In a field with low initial numbers of nematodes, the numbers of cysts and eggs in soil remained low through 100 days from planting then increased during the remainder of the growing season. In a field with high initial nematode populations, numbers increased at 30 days, decreased to low numbers at 100 days, and then resurged to maximum populations at harvest. Numbers of juveniles were greatest at 100 days in the low initial population density field and at planting in the high initial population density field. The initial numbers of eggs in the soil gave the best correlation to soil and root nematode populations 15 and 30 days later. Juveniles in the soil at planting gave the largest correlation coefficients with nematode populations in the roots at 15 days in the field with the low initial population density. Eggs and juveniles in the soil at harvest were poorly related to numbers that overwintered.
The potency of the inducers of systemic acquired resistance (SAR), acibenzolar-s-methyl, DL-alpha-amino-n-butyric acid (AABA), DL-beta-amino-n-butyric acid (BABA), gamma-amino-n-butyric acid (GABA), p-aminobenzoic acid (PABA), riboflavin, and salicylic acid (SA), in reducing reproduction of Meloidogyne javanica and Rotylenchulus reniformis in pineapple was investigated. All inducers were applied as foliar sprays to 1-mon-old pineapple plants (20 ml/plant) grown in 22-cm-diam. pots in the greenhouse. Two days after application, 10,000 eggs of M. javanica or R. reniformis were inoculated onto the plants. Six months after inoculation, nematode reproduction was measured. Acibenzolar decreased R. reniformis egg production by 58% compared to the nontreated control (P
Damage and reproductive potentials of Pratylenchus brachyurus and P. penetrans on soybean, Glycine max, cvs. Essex, Forrest, and Lee 68, were determined in microplot tests. Cultivar Essex was generally tolerant to P. brachyurus. Yield of Forrest was suppressed linearly with increasing P(i)'s in the sandy soil (r = -0.92) and loamy sand soil (r = -0.99). Low to moderate P(i)'s in the sandy clay loam gave an increase in yields as compared to plants without nematodes. Yield was not affected by this nematode in muck. Lee 68 was very sensitive to P. penetrans in microplots. Yield vs. P(i) was fitted by a quadratic model (r = 0.82) with yield decreasing sharply as P(i)'s were increased. The reproduction of both species decreased with increases in P(i). Lee 68 was a good host for P. penetrans, whereas Essex and Forrest were fair to poor hosts for P. brachyurus.
Soil samples were collected from three native Iowa prairies and analyzed for plant paiasitic nematodes and selected soil properties. Sites or nematodes were clustered with similarities related to habitat by a cluster analysis of site by nematode species and of nematodes by site. Some nematodes occurred in a wide range of prairie habitats, whereas others were more restricted. For example, greater numbers of Xiphinema americanum were in the low, well-drained sites than in the low wet sites or upland dry sites. Wet sites contained fewer nematodes than well-drained sites. Well-drained sites contained mainly Tylenchorhynchus maximus, Helicotylenchus pseudorobustus, and X. americanum. Wetter sites contained almost exclusively X. chambersi, H. hydrophilus, Telylenchus joctus, and an undescribed species of Tylenchorhynchus.
A series of greenhouse experiments was conducted to elucidate the postinfection development of Heterodera glycines in response to applications of alachlor and fenamiphos. The rate of H. glycines maturation on a susceptible soybean cultivar was not altered by 1.0 mug alachlor/g soil but was completely inhibited by 1.0 or 1.5 mug fenamiphos/g soil. An alachlor-fenamiphos combination allowed development after an initial 300-degree-day delay. Nematode maturation on the resistant soybean cultivar Centennial with 1.0 mug alachlor/g soil was similar to that observed on an untreated resistant control. Twice as many females matured on Centennial plants growing in alachlor-treated soil as on untreated Centennial plants. Fenamiphos in combination with alachlor (1.0 mug a.i./g soil) allowed development on Centennial at half the rate of the resistant control. This antagonism between alachlor and fenamiphos on development may help to explain late season population resurgence of H. glycines observed with field application of these pesticides.
The effects of temperature on rates of development of Heterodera glycines egg and juvenile stages were examined as a basis for predicting generation times of the nematode on soybean. The relationship of temperature to H. glycines embryonic development between 15 and 30 C was described by a linear model, The calculated basal temperature threshold was 5 C. Thermal optimum for embryogenesis and hatch with low mortality was 24 C. Development proceeded to first-stage juvenile at 10 C and to second-stage juvenile at 15-30 C. Hatch occurred at 20-30 C. At 36 C, development proceeded to the four-cell stage, then the eggs died. The range of diurnal soil temperature fluctuation and accumulated degree-days between 5 and 30 C (DD5/30) had an impact on rate of development of juveniles in soybean roots. From early June to early July, H. glycines required 534 + 24 DD5/30 (4 weeks) to complete a life cycle in the field. During the midseason (July and August), life cycles were completed in 3 weeks and 429 +/- 24 DD5/30 were accumulated. Late in the season (September to November), declining soil temperatures were associated with generation times of 4 weeks and slower rates of development.
