Alejandra A. López Mañanes

Consejo Nacional de Investigaciones Científicas y Técnicas

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Juana Cristina del Valle

National University of Mar del Plata

Silvina Andrea Pinoni

Consejo Nacional de Investigaciones Científicas y Técnicas

María Soledad Michiels

National University of Mar del Plata

Antonela Asaro

National University of Mar del Plata

Eugenia Méndez

National University of Mar del Plata

Adriana L. Goldemberg

National University of Mar del Plata

F.V. Vega

Universidade de Santiago de Compostela

Cristina Busch

Sana Klinikum

Gustavo Raúl Daleo

National University of Mar del Plata

Roberto A. Paggi

Consejo Nacional de Investigaciones Científicas y Técnicas

Cooperative Institutions

National University of Mar del Plata

Fundación Ciencias Exactas y Naturales

Consejo Nacional de Investigaciones Científicas y Técnicas

Institute of Marine and Coastal Research

Instituto de Investigacións Mariñas

Universidad de Cádiz

Centro Científico Tecnológico Mar del Plata

Consejo Superior de Investigaciones Científicas

Centro Científico Tecnológico - San Juan

Centro Científico Tecnológico - Tucumán

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Biochemical characteristics and modulation by external and internal factors of aminopeptidase-N activity in the hepatopancreas of a euryhaline burrowing crab

Journal of Comparative Physiology B (2015)

María Soledad Michiels Juana Cristina del Valle Alejandra A. López Mañanes

Hepatopancreas

Euryhaline

10.1007/s00360-015-0899-3

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Citations (17)

Response to environmental salinity of Na+–K+ATPase activity in individual gills of the euryhaline crab Cyrtograpsus angulatus

Journal of Experimental Marine Biology and Ecology (2002)

Alejandra A. López Mañanes C.D Meligeni Adriana L. Goldemberg

Euryhaline

10.1016/s0022-0981(02)00166-1

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Citations (29)

Feeding and osmoregulation in the euryhaline crab Neohelice granulata: digestive parameter responses

Animal Biology (2019)

Eugenia Méndez Alejandra A. López Mañanes Silvina Andrea Pinoni

Abstract When a species often experiences variation in its environmental conditions, metabolic flexibility is required. We studied the duration of the digestive cycle and the activity of key digestive enzymes (proteolytic, amylase, lipase) at short and long times after feeding in the hepatopancreas of the osmoregulator crab Neohelice granulata from the Mar Chiquita coastal lagoon (Buenos Aires Province, Argentina). We compared these responses upon hyper-regulation and under osmoconformation conditions (10 and 35 psu). The results show the ability of this crab to hyper-regulate in different feeding states. No significant differences were observed in the duration pattern of the digestive cycle between hyper-regulation and osmoconformity. However, distinct responses after feeding were observed in the activities of the digestive enzymes studied in relation to the osmoregulatory state. In individuals exposed to 35 psu, proteolytic activity was higher at 8 h and remained constant until 72 h after feeding. At 10 psu, this activity was higher at 48 h after feeding than before feeding. At 35 psu, the amylase activity after feeding was greater than the prefeeding activity. No differences were observed in the activity of lipase at 35 psu, but at 10 psu this activity was lower 1 h after feeding than before feeding. This work constitutes a contribution to our knowledge of the physiology of crustaceans and attempts to clarify the possible mechanisms of digestive settings associated with hyper-regulation.

Euryhaline

Hepatopancreas

Digestive enzyme

Osmoregulation

Digestion

10.1163/15707563-20191037

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Citations (3)

Digestive flexibility in a euryhaline crab from a SW Atlantic coastal lagoon: alkaline phosphatase activity sensitive to salinity in the hepatopancreas

Journal of the Marine Biological Association of the United Kingdom (2015)

Silvina Andrea Pinoni Eugenia Méndez Alejandra A. López Mañanes

We studied biochemical characteristics and the response to low salinity at short and long-term after feeding of alkaline phosphatase (AP) activity in hepatopancreas of the osmoregulator crab Neohelice granulata from Mar Chiquita coastal lagoon (Buenos Aires Province, Argentina) (37°32′–37°45′S 57°19′–57°26′W). The hepatopancreas exhibited a levamisole-insensitive and a levamisole-sensitive AP activity with distinct characteristics. Levamisole-insensitive activity was similar within the range of pH 7.4–9.0 and exhibited a Michaelis–Menten kinetics. Levamisole-sensitive AP activity appeared to be maximal at pH 8.5 and appeared to exhibit an allosteric kinetics. In crabs acclimated to 10 psu (hyper-regulation conditions) levamisole-insensitive and levamisole-sensitive AP activity increased (about 16-fold) over time from short term (2–4 h) to long term (120 h) after feeding while no changes occurred in crabs acclimated to 35 psu (osmoconforming conditions). The changes of AP activity along with the higher values at 120 h after feeding in 10 psu compared with those in 35 psu, and the concomitant changes in proteolytic activity, suggest a role of AP in digestive and metabolic adjustments at the biochemical level upon hyper-regulatory conditions.

Hepatopancreas

Euryhaline

Levamisole

10.1017/s0025315415000570

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Citations (10)

Seasonal changes in body composition of Ctenomys talarum (Rodentia: Octodontidae): An herbivore subterranean rodent

Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology (2006)

Juana Cristina del Valle Alejandra A. López Mañanes Cristina Busch

Burrow

Seasonality

10.1016/j.cbpa.2006.04.033

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Citations (6)

Glucose homeostasis in the euryhaline crab Cytograpsus angulatus: Effects of the salinity in the amylase, maltase and sucrase activities in the hepatopancreas and in the carbohydrate reserves in different tissues Part B Biochemistry & molecular biology

Comparative Biochemistry and Physiology (2018)

Antonela Asaro Roberto A. Paggi Juana Cristina del Valle Alejandra A. López Mañanes

Hepatopancreas

Euryhaline

Maltase

Sucrase

Source

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Citations (0)

Digestive flexibility in response to environmental salinity and temperature in the non-symbiotic sea anemone Bunodosoma zamponii

Hydrobiologia (2015)

Juana Cristina del Valle Fabián H. Acuña Alejandra A. López Mañanes

Sea anemone

Maltase

Anemone

Environmental change

10.1007/s10750-015-2239-8

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Citations (6)

Activity of Na+-K+-ATPase in a "freshwater shrimp", Palaemonetes argentinus: ontogenetic changes and effects of salinity

Romina B. Ituarte Alejandra A. López Mañanes Eduardo D. Spivak Klaus Anger

The ability of an aquatic organism to tolerate wide salinity variations without compromising life processes is termed euryhalinity. This capability requires physiological, biochemical and ecological adaptations. Strategies permitting euryhaline decapods to inhabit aquatic environments with variable salinities have been studied almost exclusively in adults. Although also larval stages have recently received an increasing attention, the salinity tolerance of embryonic stages has remained little known (1). Embryos, larvae and adults of the shrimp Palaemonetes argentinus tolerate a wide range of salinities (1-~25), but osmoregulatory capacities have been demonstrated only in post-embryonic developmental stages (2; 3). As in most other crustaceans, little has been known about osmoregulation during the embryonic phase.

Euryhaline

Osmoregulation

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Citations (0)

Digestive flexibility in the euryhaline crab Cyrtograpsus angulatus (Decapoda, Brachyura, Varunidae) from Mar Chiquita coastal lagoon (Buenos Aires, Argentina): responses to salinity of key enzymes in hepatopancreas

Silvina Andrea Pinoni Alejandra A. López Mañanes

Fil: Pinoni, Silvina Andrea. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina

Euryhaline

Hepatopancreas

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Citations (2)

In silico analysis and effects of environmental salinity in the expression and activity of digestive α-amylase and trypsins from the euryhaline crabNeohelicegranulata

Canadian Journal of Zoology (2017)

Antonela Asaro Juan António Martos-Sitcha Gonzalo Martínez‐Rodríguez Juan Miguel Mancera Alejandra A. López Mañanes

Studies on molecular characteristics and modulation of expression of α-amylase and trypsin in the hepatopancreas of intertidal euryhaline crabs are lacking. In this work, we cloned and studied by in silico approaches the characteristics of cDNA sequences for α-amylase and two trypsins isoforms, as well as the effect of environmental salinity, on gene expression and protein activities in the hepatopancreas of Neohelice granulata (Dana, 1851), which is a good invertebrate model species. The cDNA sequence of α-amylase is 1637 bp long, encoding 459 amino acid residues. Trypsin 1 and 2 are 689 and 1174 bp long, encoding 204 and 151 amino acid residues, respectively. Multiple sequence alignment of deduced protein sequences revealed the presence of conserved motifs found in other invertebrates. In crabs acclimated at 37 psu (hyporegulation), α-amylase mRNA level and total pancreatic amylase activity were higher than at 10 psu (hyperregulation) and 35 psu (osmoconformation). Trypsin 1 mRNA levels increased at 37 psu, while trypsin 2 levels decreased at 10 and 37 psu. Total trypsin activity was similar in all salinities. Our results showed a differential modulation of α-amylase and trypsin expression and total amylase activity by salinity acclimation, suggesting the occurrence of distinct mechanisms of regulation at different levels that could lead to digestive adjustments in relation to hyperregulation and (or) hyporegulation.

Euryhaline

Hepatopancreas

10.1139/cjz-2016-0324

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Citations (4)