Umeo Ito

A simple sedimentation chamber adaptable to a laboratory centrifuge is presented. The apparatus is a modified Kolar-Zeman sedimentation chamber and is designed to fit an existing centrifuge. The apparatus is available at a fraction of the cost of alternate methods and can be used in both large and small laboratories. The device should work as efficiently as a Cyto-Centrifuge with regard to quantity and morphology of cells recovered. This simple and rapid procedure is ideal for routine examination of cerebrospinal fluid for clinical diagnosis.

Centrifuge

Sedimentation

10.1093/ajcp/58.5.590

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Citations (3)

Changes in cranial and rectal temperature, blood pressure and arterial blood gas during and after unilateral and bilateral forebrain ischemia in Mongolian gerbils

Neuroscience Research (1993)

Yoji Hakamata Umeo Ito S. Hanyu Mitsuo Yoshida

10.1016/0168-0102(93)90107-2

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Citations (1)

Electron Microscopic Observation of Cerebro-Spinal Fluid Cells A New Method for Embedding of CSF Cells-

Journal of Electron Microscopy (1970)

Umeo Ito Yutaka Inaba

In this paper a new method of ours is to be described, which makes it possible to obtain the more precise electron microscopic picture of cerebrospinal fluid cells than before, by way of embedding in epoxy resin. The whole aspects of the method is that, at first CSF cells are suspended in the serum of the patient, and then are fixed with osmic acid or glutaraldehyde fixative. Consequently, the fixatives make the serum to be a solid and firm mass which can be sliced easily. This method also is available to other free cells such as in urine, ascites and pleural effusion. After prepearing of the samples by this method, several kinds of cells appearing in normal and abnormal CSF of patients of a few kinds of diseases and/or conditions of the central nervous system were observed by an electron microscope.

Fixative

Glutaraldehyde

Ascitic fluid

10.1093/oxfordjournals.jmicro.a049712

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Citations (10)

Ultrastructural Features of Astrocyte in Ischemic Edema

The Keio Journal of Medicine (1999)

Umeo Ito Toshihiko Kuroiwa S. Hanyu Yoji Hakamata Kiyomitsu Oyanagi

Brain Edema

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Temporary Focal Cerebral Ischemia Results in Swollen Astrocytic End-Feet That Compress Microvessels and Lead to Focal Cortical Infarction

Journal of Cerebral Blood Flow & Metabolism (2011)

Umeo Ito Yoji Hakamata Emiko Kawakami Kiyomitsu Oyanagi

Correction to: Journal of Cerebral Blood Flow & Metabolism (2010) 31, 328–338; doi: 10.1038/jcbfm.2010.97; published online 30 June 2010 1. Following the publication of this article, the authors determined that the article title was inaccurate. The new title is ‘Temporary cerebral ischemia results in swollen astrocytic end-feet that compress microvessels and lead to delayed focal cortical infarction.’ 2. Kiyomitsu Oyanagi's institution (3) is in ‘Nagano, Japan’.

10.1038/jcbfm.2010.144

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Citations (3)

A-106. Microneurosurgery in Cerebral Aneurysms

Neurologia medico-chirurgica (1972)

Yutaka Inaba Yoshiharu FUKUSHIMA Naoya TACHIBANA Umeo Ito A Kamisasa

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Citations (4)

Synopsis and Round Table Discussion

Springer eBooks (1997)

Umeo Ito Takaaki Kirino Toshihiko Kuroiwa I. Klatzo