Marie‐France Martin‐Eauclaire

Centre de Recherche en Neurobiologie - Neurophysiologie de Marseille

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Pierre E. Bougis

Centre National de la Recherche Scientifique

Hervé Rochat

Bioénergétique et Ingénierie des Protéines

Christian Legros

Centre National de la Recherche Scientifique

Fatima Laraba‐Djebari

University of Sciences and Technology Houari Boumediene

Brigitte Céard

Centre National de la Recherche Scientifique

J. Van Rietschoten

Centre National de la Recherche Scientifique

Marcel Crest

Laboratoire de Neurosciences Cognitives

Maria Elena de Lima

Grupo Santa Casa de Belo Horizonte

Pascal Mansuelle

Centre National de la Recherche Scientifique

Frank Bosmans

Vrije Universiteit Brussel

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Centre National de la Recherche Scientifique

148

Aix-Marseille Université

Inserm

Universidade Federal de Minas Gerais

Centre de Recherche en Neurobiologie - Neurophysiologie de Marseille

Bioénergétique et Ingénierie des Protéines

Institut de Neurobiologie de la Méditerranée

Roche (Switzerland)

Laboratoire de Neurosciences Cognitives

Laboratoire de Biochimie

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Characterization of a new peptide from Tityus serrulatus scorpion venom which is a ligand of the apamin‐binding site

FEBS Letters (1996)

Christian Legros Razika Oughuideni Hervé Darbon Hervé Rochat Pierre E. Bougis

A new ligand (Ts κ) of the apamin binding site on rat brain synaptosomes ( K 0.5 = 300 pM) was purified and characterized from the venom of Tityus serrulatus . It is a polypeptide toxin of 35 amino acid residues, with three disulfide bridges. Its cDNA was amplified from a venom gland cDNA library and the nucleotide sequence determined. A model of Ts κ was constructed by amino acid replacement using charybdotoxin structure as determined by 1 H nuclear magnetic resonance as starting model.

Apamin

Scorpion toxin

Scorpion Venoms

10.1016/0014-5793(96)00616-3

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Citations (54)

Comparison of barley malt α-amylase isozymes 1 and 2: construction of cDNA hybrids by in vivo recombination and their expression in yeast

Gene (1993)

Nathalie Juge Morten Søgaard Jean‐Claude Chaix Marie‐France Martin‐Eauclaire Birte Svensson

10.1016/0378-1119(93)90415-y

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Citations (25)

Use of fusion protein constructs to generate potent immunotherapy and protection against scorpion toxins

Vaccine (2001)

Christian Legros Hajer Kaabi M. El Ayeb Brigitte Céard Hélène Vacher

10.1016/s0264-410x(01)00379-6

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Citations (25)

Purification, amino-acid sequence and partial characterization of two toxins with anti-insect activity from the venom of the South American scorpion Tityus bahiensis (Buthidae)

Toxicon (2001)

Adriano M.C. Pimenta Marie‐France Martin‐Eauclaire Hervé Rochat Suely G. Figueiredo Evanguedes Kalapothakis

Buthidae

Scorpion Venoms

Median lethal dose

10.1016/s0041-0101(00)00240-3

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Citations (48)

Chemical synthesis and structure–activity relationships of Ts κ, a novel scorpion toxin acting on apamin‐sensitive SK channel

Journal of Peptide Research (1999)

C. Lecomte Gilles Ferrat Ziad Fajloun J. Van Rietschoten Hervé Rochat

Abstract: Tityus kappa (Ts κ), a novel toxin from the venom of the scorpion Tityus serrulatus , is a 35‐residue polypeptide cross‐linked by three disulphide bridges and acts on small‐conductance calcium‐activated potassium channels (SK channels). Ts κ was chemically synthesized using the solid‐phase method and characterized. The synthetic product, sTs κ, was indistinguishable from the natural toxin when tested in vitro in competition assay with radiolabelled apamin for binding to rat brain synaptosomes (IC 50 = 3 n m ). The sTs κ was further tested in vivo for lethal activity to mice following intracerebroventricular inoculation (LD 50 = 70 ng per mouse). The half‐cystine pairings were formerly established by enzyme‐based cleavage of sTs κ; they were between Cys 7 –Cys 28 , Cys 13 –Cys 33 and Cys 17 –Cys 35 , which is a disulphide bridge pattern similar to that of other short scorpion toxins. According to previous studies on SK channel‐acting toxins, the putative influence of certain basic residues of Ts κ (i.e. Arg 6 , Arg 9 , Lys 18 , Lys 19 ) in its pharmacological activity was investigated using synthetic point‐mutated analogues of the toxin with an Ala substitution at these positions. Data from binding assay, together with conformational analysis of the synthetic analogues by 1 H‐NMR, suggest that Arg 6 , and to a lesser extent Arg 9 , are important residues for an high‐affinity interaction of this toxin with SK channels; interestingly these residues are located outside the α‐helical structure, whereas the pharmacologically important basic residues from other SK channel‐specific toxins had been located inside the α‐helix.

Apamin

Scorpion toxin

Scorpion Venoms

10.1034/j.1399-3011.1999.00107.x

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Citations (33)

Dipole moments of scorpion toxins direct the interaction towards small- or large-conductance Ca2+-activated K+ channels

Letters in Peptide Science (1997)

Valérie Frémont Eric Blanc Marcel Crest Marie‐France Martin‐Eauclaire Maurice Gola

Helix (gastropod)

Calcium-activated potassium channel

10.1007/bf02442894

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Citations (13)

P05, a new leiurotoxin I-like scorpion toxin: Synthesis and structure-activity relationships of the .alpha.-amidated analog, a ligand of calcium-activated potassium channels with increased affinity

Biochemistry (1993)

Jean‐Marc Sabatier H. Zerrouk H. Darbon Kamel Mabrouk A. Benslimane

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTP05, a new leiurotoxin I-like scorpion toxin: Synthesis and structure-activity relationships of the .alpha.-amidated analog, a ligand of calcium-activated potassium channels with increased affinityJ. M. Sabatier, H. Zerrouk, H. Darbon, K. Mabrouk, A. Benslimane, H. Rochat, M. F. Martin-Eauclaire, and J. Van RietschotenCite this: Biochemistry 1993, 32, 11, 2763–2770Publication Date (Print):March 23, 1993Publication History Published online1 May 2002Published inissue 23 March 1993https://pubs.acs.org/doi/10.1021/bi00062a005https://doi.org/10.1021/bi00062a005research-articleACS PublicationsRequest reuse permissionsArticle Views75Altmetric-Citations78LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts

Alpha (finance)

Scorpion toxin

10.1021/bi00062a005

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Citations (96)

Solution structure of the N(1-21)MeuTXK-beta in presence of TFE

Journal of Back and Musculoskeletal Rehabilitation (2010)

Shunyi Zhu Bin Gao André Aumelas Carmen García‐Rodríguez Humberto Lanz‐Mendoza

BETA (programming language)

10.13018/bmr20075

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Citations (0)