Llanos Palop

Abstract Amyotrophic lateral sclerosis (ALS), a devastating progressive neurodegenerative disease, has no effective treatment. Recent evidence supports a strong metabolic component in ALS pathogenesis. Indeed, metabolic abnormalities in ALS correlate to disease susceptibility and progression, raising additional therapeutic targets against ALS. Ozone (O 3 ), a natural bioactive molecule, has been shown to elicit beneficial effects to reduce metabolic disturbances and improved motor behavior in TDP-43 A315T mice. However, it is fundamental to determine the mechanism through which O 3 acts in ALS. To characterize the association between O 3 exposure and disease-associated weight loss in ALS, we assessed the mRNA and protein expression profile of molecular pathways with a main role in the regulation of the metabolic homeostasis on the hypothalamus and the brown adipose tissue (BAT) at the disease end-stage, in TDP-43 A315T mice compared to age-matched WT littermates. In addition, the impact of O 3 exposure on the faecal bacterial community diversity, by Illumina sequencing, and on the neuromuscular junctions (NMJs), by confocal imaging, were analysed. Our findings suggest the effectiveness of O 3 exposure to induce metabolic effects in the hypothalamus and BAT of TDP-43 A315T mice and could be a new complementary non-pharmacological approach for ALS therapy.

Pathogenesis

10.1038/s41598-022-25033-4

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Genetic diversity (RAPD-PCR) of lactobacilli isolated from âAlmagroâ eggplant fermentations from two seasons

FEMS Microbiology Letters (2004)

Susana SeseÃ±a Isabel SÃ¡nchez Llanos Palop

Genetic diversity of 323 strains of lactobacilli isolated from an Almagro eggplant manufacturing plant was analyzed by using random amplified polymorphic DNA (RAPD). Thirty-four distinct RAPD patterns were obtained with 95% of isolates grouped into 18 main clusters. Genetic diversity was higher in brines from season II, Lactobacillus plantarum and Lactobacillus fermentum/cellobiosus being the species with the greatest number of genotypes. A single L. fermentum/cellobiosus genotype comprised isolates from both seasons and could be considered endemic to that factory.

Lactobacillus fermentum

10.1111/j.1574-6968.2004.tb09751.x

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