In the rabbit, jaw opening and closing occurs in combination with condylar protrusion and retraction. Consequently the centre of rotation (CR) for the movement lies in the inferior portion of the ascending ramus of the mandible, far below the condyle. This location is stable and independent of the type of food being chewed and the age of the animal. The topography of the soft tissues at the posterior border of the ramus is adapted to the movement pattern. The facial nerve crosses the space between skull and mandible at the level of the CR. The parotid gland lies behind the ramus between condyle and CR; below the CR, the gland is replaced by loose adipose tissue. A computer model was used to demonstrate that the location of the CR determines the amount of stretch of the large masseter and medial pterygoid muscles. Using parameters for passive elastic behaviour of muscle, obtained by postmortem measurements, it can be shown that the normal CR position minimises muscle stretch and passive elastic forces. Even a small upward displacement of the CR causes a significant amount of resistance in the jaw-closers at gapes comparable to those reached in natural mastication. A second, less dramatic effect of increased muscle stretch is a sharper decline of maximum possible active closing force, due to the interrelationship of fibre length and isometric tension. In young animals, the muscle fibres are relatively long but stiff; in adults they are shorter, but more compliant. In both ages the CR is located in such a way that masseter and medial pterygoid stretching is minimised. The high position of the temporomandibular joint ensures a maximal leverage for the muscles mentioned above. By separating the point where the reaction forces apply (the joint) from the location of the rotational axis, maximum leverage and minimal length changes of the jaw-closing muscles are achieved simultaneously. It is further suggested that elastic muscular forces play a role in determining the position of the CR.
Summary In order to obtain broader insights into the equine musculoskeletal system, we studied the fibre type composition of 2 locomotory muscles in biopsies from Dutch Warmblood foals taken at 3 different ages in the first year postpartum. The muscle fibre types were determined histochemically as well as immunohistochemically. ATPase‐characterised IIB fibres appear to express either IId or type IIa plus IId myosin heavy chain (MHC). A high percentage of fibres classified as IIA with ATPase expressed both fast types of MHC. The type I classification by the 2 methods matched almost completely. There was an increase with age of fibres expressing I and IIa MHC in the gluteus medius. At the same time, there was a decrease of fibres expressing IId MHC and fibres co‐expressing MHC IIa and IId. MHC expression of the semitendinosus muscle did not change over time at first, but from age 22–48 weeks there was a decrease in the percentage of type IId fibres. In general, the gluteus medius contained more type I fibres but fewer type IId fibres compared to the semitendinosus. At most ages the fibre type compositions of both muscles correlated with one another. To examine the effect of exercise, one‐third of the foals were given box rest, one‐third received training and one‐third kept at pasture during the first 22 weeks of life. The 3 exercise groups differed in their fibre type composition; however, these differences could not be attributed to the effect of exercise.
The complex, pennate architecture of the human masseter muscle points to a functional division into more than the commonly distinguished deep and superficial parts. In this study, the possible existence of regional differences in activation was examined. EMG activity was registered in three deep and three superficial regions with the use of bipolar fine-wire electrodes. Recordings were made during different static bite tasks, in specified directions, and with a specified bite-force magnitude. A linear bite-force/EMG relationship was observed. Furthermore, it appeared that muscle regions showed a different pattern of change in activity as a function of bite-force direction. Heterogeneity was nearly absent in anteriorly-, anteriomedially-, and medially-directed bites, but became increasingly obvious in the other bite-force directions. The posterior deep region showed the most aberrant activation pattern, which was almost opposite that from the other regions. This part was fully active in posterolaterally-directed bites. The posterior superficial region showed the largest variability in activity as a function of bite-force direction. The results point to a functional partition of the masseter muscle in at least three parts: anterior deep, posterior deep, and superficial. A further subdivision of the superficial portion might be present, but was not as obvious as the division of the deep masseter.
The cross-sectional areas of the masseter, temporalis, medial pterygoid and lateral pterygoid muscles were determined by means of computer tomography in 16 male subjects with healthy dentitions. The physiological cross-section (PCS) of these muscles was predicted from the previously determined relationship between PCS and scan cross-sections. In our subjects, mean total PCS of the jaw muscles was twice as high as in cadavers with few natural teeth. The average distribution of total PCS over the four muscles was the same in the two groups. There was considerable individual variation. Strong correlations in cross-sectional area were only found between the masseter and medial pterygoid muscles. Variation in PCS of these two muscles determines 80% of the variation in combined cross-sectional area.
1. We studied isometric twitch peak force (TPF) and twitch contraction time (TCT) of 249 motor units of the masseter muscle in 41 rabbits after extracellular electrical stimulation of single trigeminal motoneurons in the brain stem. In 41 of these units we determined the amount of tension decrease during a partially fused tetanus (sag) and the ratio between peak tetanic force after 2 min of intermittent tetanic stimulation and initial tension (fatigue index). Muscle fibers of 24 motor units were identified by the glycogen depletion method and characterized in serial sections with monoclonal antibodies against type IID, IIA, "cardiac" alpha, and I isoforms of myosin heavy chain (MHC). 2. The motor units had TCTs ranging from 13 to 32 ms. The majority of the units showed forces < 35 mN. The TPFs were larger and varied more for motor units with short and intermediate TCTs than for units with long TCTs. There is a small but statistically significant negative correlation between the motor unit TPF and the TCT. 3. All units exhibited "sag" and, with the exeption of one, had fatigue indexes > 0.75. The studied rabbit masseter motor units can therefore be classified as fast, fatigue-resistant, except for one that belonged to the FF (fast, fatigable) category. No slow units were represented in the sample pool. Significant correlations were not found either between TCT and the amount of sag or between TCT and the fatigue index. 4. Immunohistochemical analysis showed that the FF unit had fibers containing only IID-MHC. Five other units were found with a single MHC--three with IIA-MHC and two with alpha-MHC. In three other units all fibers showed one combination of two MHCs (1 IIA/IID, 1 IIA/alpha, and 1 alpha/I). The remaining 15 units contained two MHCs spread unevenly over the constituting fibers. Large variations in myosin composition of fibers within one motor unit cast doubts on the presumed dominant neuronal influence on myosin expression in the adult animal. 5. We found a close, statistically significant correlation between the TCT and the estimated MHC content of the units: the TCT was 13 ms for the IID unit, 18 ms for the pure IIA units, and 28 ms for the pure alpha units. Units with two MHCs had intermediate TCTs; units with alpha/I-MHC mixtures had TCTs of 29-30 ms. No pure MHC-I units were identified.(ABSTRACT TRUNCATED AT 400 WORDS)
The myosin heavy chain (MHC) content and spatial distribution of the fibers of 11 motor units (MUs) of the rabbit masseter muscle were determined. The fibers of single MUs were visualized in whole-muscle serial sections by a negative periodic acid/Schiff reaction for glycogen after they had been depleted of glycogen by extracellular stimulation of their motoneuron in the trigeminal motor nucleus. The MHC isoforms present in the fibers were characterized by monoclonal antibodies. Individual fibers appeared to contain from one to three MHC isoforms. In six cases, all fibers of a motor unit had an identical MHC content; in five cases, different fiber types were found in a single unit. The fiber number per MU varied between 40 and 424, the territory size between 1.1 and 11.0 mm2 (of a total muscle cross-section of 200 mm2), and fiber density between 6 and 17 MU fibers per 100 muscle fibers. In the multipennate masseter, the fibers were usually restricted to a single anatomical compartment. In comparison with leg muscles, the fibers of the masseter motor units, although similar in number, were restricted to relatively smaller subvolumes of the muscle and thus reached higher densities in their territories. The small territories are the anatomical substrate for the observed heterogeneity of motor behavior. Since the different anatomical compartments of the masseter differ with respect to their biomechanical capabilities, this makes this muscle multifunctional in the exertion of complex motor tasks.
From knee extension moments measured with a dynamometer, the quadriceps muscle force, the patellar ligament force and the reaction force in the patellofemoral joint at various knee angles (0-90 degrees) were estimated. The information needed to calculate the combined effect of both patellofemoral and tibiofemoral joint on the mechanical advantage of the muscle was obtained from lateral-view radiographs of autopsy knees. The results show that the smallest quadriceps force (2,000 N) is exerted at maximal extension, and the largest force (8,000 N) at about 75 degrees of flexion. The patellar ligament force reaches a maximum (5,000 N) at 60 degrees. The reaction force in the patellofemoral joint is the smallest (1,000 N) at extension and is of the same values as the muscle force in a range from 75 to 90 degrees. Especially at large flexion angles, the value of the estimated forces is considerably larger (by 100%) than reported in the literature. This difference is attributed to the influence of the patellofemoral joint on the mechanical advantage of the muscle, which has not been taken into account in other studies.
